Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12385 and BWI76_RS12390 are separated by 135 nucleotides BWI76_RS12390 and BWI76_RS12395 are separated by 349 nucleotides BWI76_RS12395 and BWI76_RS12400 are separated by 59 nucleotides
BWI76_RS12385: BWI76_RS12385 - DNA polymerase V subunit UmuC, at 2,586,868 to 2,588,133
_RS12385
BWI76_RS12390: BWI76_RS12390 - antibiotic biosynthesis monooxygenase, at 2,588,269 to 2,588,568
_RS12390
BWI76_RS12395: BWI76_RS12395 - zinc-binding alcohol dehydrogenase, at 2,588,918 to 2,589,901
_RS12395
BWI76_RS12400: BWI76_RS12400 - TetR family transcriptional regulator, at 2,589,961 to 2,590,617
_RS12400
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2587.963 kb on + strand, within BWI76_RS12385 at 2588.096 kb on + strand at 2588.104 kb on - strand at 2588.116 kb on - strand at 2588.230 kb on - strand at 2588.365 kb on + strand at 2588.435 kb on + strand, within BWI76_RS12390 at 2588.494 kb on + strand, within BWI76_RS12390 at 2588.624 kb on - strand at 2588.624 kb on - strand at 2588.649 kb on - strand at 2588.675 kb on - strand at 2588.710 kb on + strand at 2588.951 kb on + strand at 2588.994 kb on - strand at 2589.188 kb on - strand, within BWI76_RS12395 at 2589.353 kb on + strand, within BWI76_RS12395 at 2589.381 kb on - strand, within BWI76_RS12395 at 2589.495 kb on + strand, within BWI76_RS12395 at 2589.581 kb on + strand, within BWI76_RS12395 at 2589.605 kb on + strand, within BWI76_RS12395 at 2589.660 kb on + strand, within BWI76_RS12395 at 2589.660 kb on + strand, within BWI76_RS12395 at 2589.660 kb on + strand, within BWI76_RS12395 at 2589.661 kb on - strand, within BWI76_RS12395 at 2589.668 kb on - strand, within BWI76_RS12395 at 2589.678 kb on - strand, within BWI76_RS12395 at 2589.687 kb on + strand, within BWI76_RS12395 at 2589.702 kb on + strand, within BWI76_RS12395 at 2589.708 kb on + strand, within BWI76_RS12395 at 2589.718 kb on + strand, within BWI76_RS12395 at 2589.750 kb on + strand, within BWI76_RS12395 at 2589.781 kb on - strand, within BWI76_RS12395 at 2589.789 kb on + strand, within BWI76_RS12395 at 2589.790 kb on + strand, within BWI76_RS12395 at 2589.797 kb on - strand, within BWI76_RS12395 at 2589.805 kb on + strand at 2589.807 kb on - strand at 2589.810 kb on + strand at 2589.813 kb on + strand at 2589.813 kb on - strand at 2589.815 kb on + strand at 2589.815 kb on - strand at 2589.822 kb on - strand at 2589.834 kb on + strand at 2589.854 kb on + strand at 2589.910 kb on + strand at 2589.910 kb on + strand at 2589.922 kb on + strand at 2589.956 kb on + strand at 2589.956 kb on + strand at 2589.964 kb on + strand at 2589.966 kb on + strand at 2589.972 kb on - strand at 2589.972 kb on - strand at 2589.972 kb on - strand at 2589.974 kb on - strand at 2589.979 kb on - strand at 2589.989 kb on + strand at 2590.005 kb on - strand at 2590.039 kb on - strand, within BWI76_RS12400 at 2590.039 kb on - strand, within BWI76_RS12400 at 2590.044 kb on - strand, within BWI76_RS12400 at 2590.085 kb on - strand, within BWI76_RS12400 at 2590.086 kb on - strand, within BWI76_RS12400 at 2590.201 kb on - strand, within BWI76_RS12400 at 2590.238 kb on - strand, within BWI76_RS12400 at 2590.340 kb on - strand, within BWI76_RS12400 at 2590.353 kb on - strand, within BWI76_RS12400 at 2590.362 kb on - strand at 2590.407 kb on - strand, within BWI76_RS12400 at 2590.524 kb on + strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.613 kb on + strand at 2590.621 kb on - strand at 2590.621 kb on - strand at 2590.679 kb on - strand at 2590.743 kb on + strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.757 kb on + strand at 2590.765 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.779 kb on - strand at 2590.781 kb on - strand at 2590.791 kb on - strand at 2590.801 kb on + strand at 2590.814 kb on + strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.824 kb on + strand at 2590.835 kb on + strand at 2590.859 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 2,587,963 + BWI76_RS12385 0.86 +0.3 2,588,096 + +0.7 2,588,104 - +1.9 2,588,116 - -0.3 2,588,230 - -0.9 2,588,365 + -1.5 2,588,435 + BWI76_RS12390 0.55 -0.1 2,588,494 + BWI76_RS12390 0.75 -0.1 2,588,624 - +0.3 2,588,624 - -1.7 2,588,649 - -0.1 2,588,675 - -0.7 2,588,710 + -2.1 2,588,951 + -0.7 2,588,994 - -0.3 2,589,188 - BWI76_RS12395 0.27 -1.1 2,589,353 + BWI76_RS12395 0.44 -0.5 2,589,381 - BWI76_RS12395 0.47 -0.5 2,589,495 + BWI76_RS12395 0.59 -0.0 2,589,581 + BWI76_RS12395 0.67 -0.4 2,589,605 + BWI76_RS12395 0.70 -1.0 2,589,660 + BWI76_RS12395 0.75 -0.5 2,589,660 + BWI76_RS12395 0.75 +0.3 2,589,660 + BWI76_RS12395 0.75 +0.7 2,589,661 - BWI76_RS12395 0.76 +0.5 2,589,668 - BWI76_RS12395 0.76 -0.4 2,589,678 - BWI76_RS12395 0.77 +0.6 2,589,687 + BWI76_RS12395 0.78 +0.1 2,589,702 + BWI76_RS12395 0.80 -1.3 2,589,708 + BWI76_RS12395 0.80 +0.3 2,589,718 + BWI76_RS12395 0.81 -0.6 2,589,750 + BWI76_RS12395 0.85 -0.3 2,589,781 - BWI76_RS12395 0.88 -0.9 2,589,789 + BWI76_RS12395 0.89 -0.0 2,589,790 + BWI76_RS12395 0.89 +0.4 2,589,797 - BWI76_RS12395 0.89 -3.0 2,589,805 + -0.1 2,589,807 - +0.9 2,589,810 + -0.3 2,589,813 + -0.5 2,589,813 - -0.7 2,589,815 + -0.8 2,589,815 - -1.5 2,589,822 - -0.1 2,589,834 + +1.5 2,589,854 + -0.4 2,589,910 + +0.0 2,589,910 + -0.8 2,589,922 + -0.4 2,589,956 + -0.4 2,589,956 + +0.3 2,589,964 + +0.5 2,589,966 + -1.1 2,589,972 - -0.0 2,589,972 - -0.3 2,589,972 - -1.2 2,589,974 - +0.2 2,589,979 - +0.3 2,589,989 + -1.7 2,590,005 - +0.7 2,590,039 - BWI76_RS12400 0.12 -0.7 2,590,039 - BWI76_RS12400 0.12 -0.2 2,590,044 - BWI76_RS12400 0.13 +0.2 2,590,085 - BWI76_RS12400 0.19 -0.3 2,590,086 - BWI76_RS12400 0.19 +0.6 2,590,201 - BWI76_RS12400 0.37 +1.1 2,590,238 - BWI76_RS12400 0.42 +1.0 2,590,340 - BWI76_RS12400 0.58 +0.4 2,590,353 - BWI76_RS12400 0.60 +0.0 2,590,362 - +0.5 2,590,407 - BWI76_RS12400 0.68 -1.2 2,590,524 + BWI76_RS12400 0.86 +0.4 2,590,532 - BWI76_RS12400 0.87 -0.1 2,590,532 - BWI76_RS12400 0.87 -0.2 2,590,532 - BWI76_RS12400 0.87 +0.4 2,590,532 - BWI76_RS12400 0.87 +0.8 2,590,532 - BWI76_RS12400 0.87 +0.7 2,590,613 + +0.9 2,590,621 - +0.1 2,590,621 - -0.5 2,590,679 - -0.5 2,590,743 + -1.0 2,590,751 - +0.2 2,590,751 - -0.4 2,590,751 - -0.4 2,590,751 - -1.7 2,590,757 + -0.1 2,590,765 - -2.8 2,590,775 - +0.4 2,590,775 - +0.2 2,590,775 - +0.3 2,590,775 - -0.2 2,590,779 - -0.5 2,590,781 - -0.4 2,590,791 - -1.3 2,590,801 + +1.3 2,590,814 + -0.5 2,590,822 - -0.2 2,590,822 - -4.6 2,590,822 - -0.8 2,590,822 - -0.3 2,590,822 - -0.3 2,590,824 + +1.2 2,590,835 + -0.0 2,590,859 + +0.3
Or see this region's nucleotide sequence