Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11830 and BWI76_RS11835 are separated by 77 nucleotides BWI76_RS11835 and BWI76_RS11840 are separated by 32 nucleotides BWI76_RS11840 and BWI76_RS11845 overlap by 1 nucleotides
BWI76_RS11830: BWI76_RS11830 - hypothetical protein, at 2,487,447 to 2,488,394
_RS11830
BWI76_RS11835: BWI76_RS11835 - putative regulatory protein MarR, at 2,488,472 to 2,488,927
_RS11835
BWI76_RS11840: BWI76_RS11840 - tryptophan synthase subunit alpha, at 2,488,960 to 2,489,769
_RS11840
BWI76_RS11845: BWI76_RS11845 - tryptophan synthase subunit beta, at 2,489,769 to 2,490,962
_RS11845
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2487.964 kb on + strand, within BWI76_RS11830 at 2488.110 kb on + strand, within BWI76_RS11830 at 2488.133 kb on + strand, within BWI76_RS11830 at 2488.242 kb on + strand, within BWI76_RS11830 at 2488.718 kb on + strand, within BWI76_RS11835 at 2488.873 kb on - strand, within BWI76_RS11835 at 2489.110 kb on + strand, within BWI76_RS11840 at 2489.118 kb on - strand, within BWI76_RS11840 at 2489.335 kb on - strand, within BWI76_RS11840 at 2489.732 kb on + strand at 2489.811 kb on + strand at 2490.020 kb on - strand, within BWI76_RS11845 at 2490.126 kb on + strand, within BWI76_RS11845
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 2,487,964 + BWI76_RS11830 0.55 +0.1 2,488,110 + BWI76_RS11830 0.70 -1.7 2,488,133 + BWI76_RS11830 0.72 -0.1 2,488,242 + BWI76_RS11830 0.84 -0.5 2,488,718 + BWI76_RS11835 0.54 +0.2 2,488,873 - BWI76_RS11835 0.88 +0.5 2,489,110 + BWI76_RS11840 0.19 -1.4 2,489,118 - BWI76_RS11840 0.20 -1.8 2,489,335 - BWI76_RS11840 0.46 -1.1 2,489,732 + -2.6 2,489,811 + -3.3 2,490,020 - BWI76_RS11845 0.21 -0.4 2,490,126 + BWI76_RS11845 0.30 -2.5
Or see this region's nucleotide sequence