Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03075 and BWI76_RS03080 are separated by 196 nucleotides BWI76_RS03080 and BWI76_RS03085 are separated by 151 nucleotides
BWI76_RS03075: BWI76_RS03075 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) <small>(from data)</small>, at 630,724 to 631,533
_RS03075
BWI76_RS03080: BWI76_RS03080 - (Fe-S)-cluster assembly protein, at 631,730 to 632,587
_RS03080
BWI76_RS03085: BWI76_RS03085 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) <small>(from data)</small>, at 632,739 to 634,652
_RS03085
Position (kb) 631
632
633 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 630.819 kb on - strand, within BWI76_RS03075 at 630.869 kb on + strand, within BWI76_RS03075 at 630.899 kb on - strand, within BWI76_RS03075 at 630.926 kb on + strand, within BWI76_RS03075 at 630.990 kb on + strand, within BWI76_RS03075 at 630.990 kb on + strand, within BWI76_RS03075 at 630.998 kb on - strand, within BWI76_RS03075 at 631.193 kb on - strand, within BWI76_RS03075 at 631.350 kb on + strand, within BWI76_RS03075 at 631.367 kb on + strand, within BWI76_RS03075 at 631.471 kb on + strand at 631.508 kb on + strand at 631.639 kb on - strand at 631.711 kb on + strand at 631.852 kb on + strand, within BWI76_RS03080 at 631.860 kb on - strand, within BWI76_RS03080 at 631.932 kb on + strand, within BWI76_RS03080 at 632.081 kb on - strand, within BWI76_RS03080 at 632.086 kb on - strand, within BWI76_RS03080 at 632.231 kb on - strand, within BWI76_RS03080 at 632.591 kb on - strand at 632.629 kb on - strand at 632.758 kb on - strand at 632.771 kb on + strand at 632.771 kb on + strand at 632.771 kb on + strand at 632.839 kb on + strand at 632.928 kb on + strand at 632.928 kb on + strand at 633.035 kb on + strand, within BWI76_RS03085 at 633.035 kb on + strand, within BWI76_RS03085 at 633.281 kb on + strand, within BWI76_RS03085 at 633.286 kb on + strand, within BWI76_RS03085 at 633.289 kb on - strand, within BWI76_RS03085
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 630,819 - BWI76_RS03075 0.12 -0.3 630,869 + BWI76_RS03075 0.18 +0.7 630,899 - BWI76_RS03075 0.22 -0.5 630,926 + BWI76_RS03075 0.25 +2.7 630,990 + BWI76_RS03075 0.33 -0.6 630,990 + BWI76_RS03075 0.33 +0.3 630,998 - BWI76_RS03075 0.34 -0.9 631,193 - BWI76_RS03075 0.58 -0.1 631,350 + BWI76_RS03075 0.77 +0.8 631,367 + BWI76_RS03075 0.79 -0.4 631,471 + +0.1 631,508 + -0.0 631,639 - -0.0 631,711 + +0.2 631,852 + BWI76_RS03080 0.14 -0.2 631,860 - BWI76_RS03080 0.15 -0.9 631,932 + BWI76_RS03080 0.24 -2.8 632,081 - BWI76_RS03080 0.41 -0.9 632,086 - BWI76_RS03080 0.41 -1.7 632,231 - BWI76_RS03080 0.58 -0.5 632,591 - -0.1 632,629 - -2.0 632,758 - +0.6 632,771 + +0.0 632,771 + -0.9 632,771 + -0.6 632,839 + +0.4 632,928 + +0.2 632,928 + -0.2 633,035 + BWI76_RS03085 0.15 +0.3 633,035 + BWI76_RS03085 0.15 +1.6 633,281 + BWI76_RS03085 0.28 -0.0 633,286 + BWI76_RS03085 0.29 -0.2 633,289 - BWI76_RS03085 0.29 -0.3
Or see this region's nucleotide sequence