Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01660 and BWI76_RS01665 are separated by 31 nucleotides BWI76_RS01665 and BWI76_RS01670 are separated by 101 nucleotides
BWI76_RS01660: BWI76_RS01660 - malate synthase A, at 336,918 to 338,519
_RS01660
BWI76_RS01665: BWI76_RS01665 - Isocitrate lyase (EC 4.1.3.1) <small>(from data)</small>, at 338,551 to 339,855
_RS01665
BWI76_RS01670: BWI76_RS01670 - bifunctional isocitrate dehydrogenase kinase/phosphatase, at 339,957 to 341,699
_RS01670
Position (kb) 338
339
340 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 337.629 kb on - strand, within BWI76_RS01660 at 337.650 kb on - strand, within BWI76_RS01660 at 337.717 kb on + strand, within BWI76_RS01660 at 337.826 kb on + strand, within BWI76_RS01660 at 337.870 kb on - strand, within BWI76_RS01660 at 338.149 kb on - strand, within BWI76_RS01660 at 338.334 kb on + strand, within BWI76_RS01660 at 338.609 kb on + strand at 339.333 kb on - strand, within BWI76_RS01665 at 339.344 kb on + strand, within BWI76_RS01665 at 339.487 kb on + strand, within BWI76_RS01665 at 339.493 kb on + strand, within BWI76_RS01665 at 339.493 kb on + strand, within BWI76_RS01665 at 339.671 kb on - strand, within BWI76_RS01665 at 339.781 kb on - strand at 339.811 kb on - strand at 339.820 kb on + strand at 339.820 kb on + strand at 339.820 kb on + strand at 339.975 kb on - strand at 340.081 kb on + strand at 340.113 kb on - strand at 340.198 kb on + strand, within BWI76_RS01670 at 340.211 kb on - strand, within BWI76_RS01670 at 340.285 kb on + strand, within BWI76_RS01670 at 340.494 kb on - strand, within BWI76_RS01670 at 340.701 kb on + strand, within BWI76_RS01670 at 340.757 kb on + strand, within BWI76_RS01670 at 340.785 kb on + strand, within BWI76_RS01670 at 340.805 kb on + strand, within BWI76_RS01670
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 337,629 - BWI76_RS01660 0.44 -1.5 337,650 - BWI76_RS01660 0.46 -2.7 337,717 + BWI76_RS01660 0.50 -1.9 337,826 + BWI76_RS01660 0.57 -2.8 337,870 - BWI76_RS01660 0.59 -2.9 338,149 - BWI76_RS01660 0.77 -4.6 338,334 + BWI76_RS01660 0.88 -3.0 338,609 + -2.0 339,333 - BWI76_RS01665 0.60 -2.3 339,344 + BWI76_RS01665 0.61 -2.5 339,487 + BWI76_RS01665 0.72 -1.6 339,493 + BWI76_RS01665 0.72 +0.2 339,493 + BWI76_RS01665 0.72 -1.5 339,671 - BWI76_RS01665 0.86 -4.1 339,781 - -2.5 339,811 - -3.2 339,820 + -2.8 339,820 + -2.4 339,820 + -2.7 339,975 - -2.6 340,081 + -2.9 340,113 - -1.3 340,198 + BWI76_RS01670 0.14 -2.5 340,211 - BWI76_RS01670 0.15 -2.9 340,285 + BWI76_RS01670 0.19 -1.1 340,494 - BWI76_RS01670 0.31 -2.9 340,701 + BWI76_RS01670 0.43 -1.5 340,757 + BWI76_RS01670 0.46 -3.5 340,785 + BWI76_RS01670 0.48 -2.3 340,805 + BWI76_RS01670 0.49 -3.0
Or see this region's nucleotide sequence