Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01545 and BWI76_RS01550 overlap by 8 nucleotides BWI76_RS01550 and BWI76_RS01555 overlap by 1 nucleotides BWI76_RS01555 and BWI76_RS01560 are separated by 253 nucleotides BWI76_RS01560 and BWI76_RS01565 are separated by 95 nucleotides
BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395
_RS01545
BWI76_RS01550: BWI76_RS01550 - thiamine phosphate synthase, at 315,388 to 316,023
_RS01550
BWI76_RS01555: BWI76_RS01555 - phosphomethylpyrimidine synthase ThiC, at 316,023 to 317,918
_RS01555
BWI76_RS01560: BWI76_RS01560 - Rsd/AlgQ family anti-sigma factor, at 318,172 to 318,669
_RS01560
BWI76_RS01565: BWI76_RS01565 - NADH pyrophosphatase, at 318,765 to 319,538
_RS01565
Position (kb) 316
317
318 Strain fitness (log2 ratio) -2
-1
0
1 at 315.138 kb on - strand, within BWI76_RS01545 at 315.202 kb on - strand, within BWI76_RS01545 at 315.435 kb on + strand at 315.461 kb on + strand, within BWI76_RS01550 at 315.551 kb on - strand, within BWI76_RS01550 at 315.725 kb on - strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.912 kb on + strand, within BWI76_RS01550 at 316.029 kb on - strand at 316.138 kb on - strand at 316.326 kb on + strand, within BWI76_RS01555 at 316.421 kb on + strand, within BWI76_RS01555 at 316.529 kb on + strand, within BWI76_RS01555 at 316.529 kb on + strand, within BWI76_RS01555 at 316.537 kb on - strand, within BWI76_RS01555 at 316.537 kb on - strand, within BWI76_RS01555 at 316.766 kb on - strand, within BWI76_RS01555 at 316.939 kb on + strand, within BWI76_RS01555 at 317.373 kb on + strand, within BWI76_RS01555 at 317.373 kb on + strand, within BWI76_RS01555 at 317.457 kb on - strand, within BWI76_RS01555 at 317.594 kb on - strand, within BWI76_RS01555 at 317.677 kb on + strand, within BWI76_RS01555 at 317.756 kb on + strand at 317.813 kb on + strand at 317.849 kb on + strand at 317.946 kb on - strand at 317.990 kb on - strand at 318.007 kb on + strand at 318.040 kb on - strand at 318.040 kb on - strand at 318.142 kb on + strand at 318.176 kb on - strand at 318.199 kb on + strand at 318.215 kb on - strand at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.483 kb on - strand, within BWI76_RS01560 at 318.564 kb on + strand, within BWI76_RS01560 at 318.605 kb on - strand, within BWI76_RS01560 at 318.630 kb on + strand at 318.638 kb on - strand at 318.668 kb on - strand at 318.754 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 315,138 - BWI76_RS01545 0.66 -0.8 315,202 - BWI76_RS01545 0.74 -1.6 315,435 + -1.2 315,461 + BWI76_RS01550 0.11 -1.7 315,551 - BWI76_RS01550 0.26 -0.9 315,725 - BWI76_RS01550 0.53 -2.6 315,779 + BWI76_RS01550 0.61 -0.6 315,779 + BWI76_RS01550 0.61 -0.9 315,779 + BWI76_RS01550 0.61 -1.7 315,912 + BWI76_RS01550 0.82 -1.0 316,029 - -1.2 316,138 - -0.2 316,326 + BWI76_RS01555 0.16 -0.7 316,421 + BWI76_RS01555 0.21 -0.7 316,529 + BWI76_RS01555 0.27 -0.5 316,529 + BWI76_RS01555 0.27 -1.0 316,537 - BWI76_RS01555 0.27 -1.6 316,537 - BWI76_RS01555 0.27 -1.2 316,766 - BWI76_RS01555 0.39 -1.5 316,939 + BWI76_RS01555 0.48 -1.1 317,373 + BWI76_RS01555 0.71 -0.9 317,373 + BWI76_RS01555 0.71 +1.6 317,457 - BWI76_RS01555 0.76 -0.4 317,594 - BWI76_RS01555 0.83 -0.6 317,677 + BWI76_RS01555 0.87 -1.3 317,756 + -0.9 317,813 + -1.4 317,849 + -0.6 317,946 - -1.3 317,990 - -1.1 318,007 + -1.0 318,040 - -0.7 318,040 - -0.8 318,142 + -1.7 318,176 - -0.2 318,199 + -2.1 318,215 - -0.3 318,371 + BWI76_RS01560 0.40 +1.5 318,371 + BWI76_RS01560 0.40 +0.9 318,371 + BWI76_RS01560 0.40 -0.5 318,379 - BWI76_RS01560 0.42 -0.2 318,379 - BWI76_RS01560 0.42 -0.1 318,483 - BWI76_RS01560 0.62 -0.7 318,564 + BWI76_RS01560 0.79 +0.1 318,605 - BWI76_RS01560 0.87 +0.3 318,630 + +0.7 318,638 - -0.1 318,668 - +1.3 318,754 + +0.7
Or see this region's nucleotide sequence