Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01420 and BWI76_RS01425 overlap by 4 nucleotides BWI76_RS01425 and BWI76_RS01430 are separated by 25 nucleotides BWI76_RS01430 and BWI76_RS01435 are separated by 438 nucleotides BWI76_RS01435 and BWI76_RS01440 are separated by 10 nucleotides BWI76_RS01440 and BWI76_RS01445 are separated by 119 nucleotides BWI76_RS01445 and BWI76_RS01450 are separated by 5 nucleotides
BWI76_RS01420: BWI76_RS01420 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 290,636 to 291,664
_RS01420
BWI76_RS01425: BWI76_RS01425 - bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor, at 291,661 to 292,623
_RS01425
BWI76_RS01430: BWI76_RS01430 - type I pantothenate kinase, at 292,649 to 293,599
_RS01430
BWI76_RS01435: BWI76_RS01435 - tRNA-Thr, at 294,038 to 294,113
_RS01435
BWI76_RS01440: BWI76_RS01440 - tRNA-Tyr, at 294,124 to 294,208
_RS01440
BWI76_RS01445: BWI76_RS01445 - tRNA-Gly, at 294,328 to 294,402
_RS01445
BWI76_RS01450: BWI76_RS01450 - tRNA-Thr, at 294,408 to 294,483
_RS01450
Position (kb)
292
293
294 Strain fitness (log2 ratio)
-2
-1
0
1 at 292.602 kb on + strand at 293.610 kb on - strand at 293.649 kb on - strand at 293.650 kb on - strand at 293.683 kb on - strand at 293.688 kb on - strand at 293.749 kb on + strand at 293.749 kb on + strand at 293.749 kb on + strand at 293.749 kb on + strand at 293.766 kb on + strand at 293.769 kb on + strand at 293.777 kb on - strand at 293.796 kb on + strand at 293.798 kb on - strand at 293.819 kb on - strand at 293.819 kb on - strand at 293.862 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 292,602 + +0.7 293,610 - +0.1 293,649 - -0.2 293,650 - +0.2 293,683 - +0.9 293,688 - -0.1 293,749 + +0.6 293,749 + -0.3 293,749 + -0.0 293,749 + -0.0 293,766 + -0.8 293,769 + -2.1 293,777 - -0.3 293,796 + -0.3 293,798 - +0.3 293,819 - -0.2 293,819 - -0.6 293,862 + +0.8
Or see this region's nucleotide sequence