Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01430

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01420 and BWI76_RS01425 overlap by 4 nucleotidesBWI76_RS01425 and BWI76_RS01430 are separated by 25 nucleotidesBWI76_RS01430 and BWI76_RS01435 are separated by 438 nucleotidesBWI76_RS01435 and BWI76_RS01440 are separated by 10 nucleotidesBWI76_RS01440 and BWI76_RS01445 are separated by 119 nucleotidesBWI76_RS01445 and BWI76_RS01450 are separated by 5 nucleotides BWI76_RS01420: BWI76_RS01420 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 290,636 to 291,664 _RS01420 BWI76_RS01425: BWI76_RS01425 - bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor, at 291,661 to 292,623 _RS01425 BWI76_RS01430: BWI76_RS01430 - type I pantothenate kinase, at 292,649 to 293,599 _RS01430 BWI76_RS01435: BWI76_RS01435 - tRNA-Thr, at 294,038 to 294,113 _RS01435 BWI76_RS01440: BWI76_RS01440 - tRNA-Tyr, at 294,124 to 294,208 _RS01440 BWI76_RS01445: BWI76_RS01445 - tRNA-Gly, at 294,328 to 294,402 _RS01445 BWI76_RS01450: BWI76_RS01450 - tRNA-Thr, at 294,408 to 294,483 _RS01450 Position (kb) 292 293 294Strain fitness (log2 ratio) -2 -1 0 1at 292.602 kb on + strandat 293.610 kb on - strandat 293.649 kb on - strandat 293.650 kb on - strandat 293.683 kb on - strandat 293.688 kb on - strandat 293.749 kb on + strandat 293.749 kb on + strandat 293.749 kb on + strandat 293.749 kb on + strandat 293.766 kb on + strandat 293.769 kb on + strandat 293.777 kb on - strandat 293.796 kb on + strandat 293.798 kb on - strandat 293.819 kb on - strandat 293.819 kb on - strandat 293.862 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
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292,602 + +0.7
293,610 - +0.1
293,649 - -0.2
293,650 - +0.2
293,683 - +0.9
293,688 - -0.1
293,749 + +0.6
293,749 + -0.3
293,749 + -0.0
293,749 + -0.0
293,766 + -0.8
293,769 + -2.1
293,777 - -0.3
293,796 + -0.3
293,798 - +0.3
293,819 - -0.2
293,819 - -0.6
293,862 + +0.8

Or see this region's nucleotide sequence