Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00840 and BWI76_RS00845 are separated by 184 nucleotides BWI76_RS00845 and BWI76_RS00850 are separated by 9 nucleotides BWI76_RS00850 and BWI76_RS00855 are separated by 77 nucleotides
BWI76_RS00840: BWI76_RS00840 - acetylornithine deacetylase, at 173,585 to 174,736
_RS00840
BWI76_RS00845: BWI76_RS00845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 174,921 to 175,925
_RS00845
BWI76_RS00850: BWI76_RS00850 - acetylglutamate kinase, at 175,935 to 176,708
_RS00850
BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159
_RS00855
Position (kb) 174
175
176 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 173.960 kb on - strand, within BWI76_RS00840 at 173.960 kb on - strand, within BWI76_RS00840 at 174.257 kb on + strand, within BWI76_RS00840 at 174.352 kb on + strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.453 kb on - strand, within BWI76_RS00840 at 174.497 kb on - strand, within BWI76_RS00840 at 174.674 kb on + strand at 174.707 kb on - strand at 174.966 kb on + strand at 174.996 kb on + strand at 175.207 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.329 kb on - strand, within BWI76_RS00845 at 175.483 kb on - strand, within BWI76_RS00845 at 175.498 kb on - strand, within BWI76_RS00845 at 175.593 kb on + strand, within BWI76_RS00845 at 175.709 kb on - strand, within BWI76_RS00845 at 175.714 kb on - strand, within BWI76_RS00845 at 175.739 kb on + strand, within BWI76_RS00845 at 175.775 kb on - strand, within BWI76_RS00845 at 175.803 kb on + strand, within BWI76_RS00845 at 175.825 kb on - strand at 176.129 kb on + strand, within BWI76_RS00850 at 176.171 kb on + strand, within BWI76_RS00850 at 176.235 kb on - strand, within BWI76_RS00850 at 176.263 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.358 kb on - strand, within BWI76_RS00850 at 176.583 kb on - strand, within BWI76_RS00850 at 176.612 kb on - strand, within BWI76_RS00850 at 176.652 kb on + strand at 176.655 kb on - strand at 176.669 kb on + strand at 176.669 kb on + strand at 176.737 kb on + strand at 176.861 kb on + strand at 176.876 kb on + strand at 176.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 173,960 - BWI76_RS00840 0.33 -0.4 173,960 - BWI76_RS00840 0.33 -1.2 174,257 + BWI76_RS00840 0.58 -0.3 174,352 + BWI76_RS00840 0.67 -0.6 174,432 - BWI76_RS00840 0.74 +0.2 174,432 - BWI76_RS00840 0.74 -0.3 174,445 + BWI76_RS00840 0.75 -0.2 174,445 + BWI76_RS00840 0.75 -0.6 174,453 - BWI76_RS00840 0.75 -0.3 174,497 - BWI76_RS00840 0.79 -0.0 174,674 + -0.2 174,707 - -0.1 174,966 + -1.4 174,996 + -0.6 175,207 + BWI76_RS00845 0.28 -2.8 175,291 + BWI76_RS00845 0.37 -2.7 175,291 + BWI76_RS00845 0.37 +0.1 175,329 - BWI76_RS00845 0.41 -2.2 175,483 - BWI76_RS00845 0.56 -2.2 175,498 - BWI76_RS00845 0.57 -0.1 175,593 + BWI76_RS00845 0.67 -3.6 175,709 - BWI76_RS00845 0.78 -1.8 175,714 - BWI76_RS00845 0.79 -2.4 175,739 + BWI76_RS00845 0.81 -0.4 175,775 - BWI76_RS00845 0.85 -0.4 175,803 + BWI76_RS00845 0.88 -3.0 175,825 - -2.5 176,129 + BWI76_RS00850 0.25 -3.9 176,171 + BWI76_RS00850 0.30 -2.9 176,235 - BWI76_RS00850 0.39 -2.8 176,263 + BWI76_RS00850 0.42 -2.6 176,350 + BWI76_RS00850 0.54 -1.7 176,350 + BWI76_RS00850 0.54 -2.2 176,358 - BWI76_RS00850 0.55 -1.4 176,583 - BWI76_RS00850 0.84 -2.8 176,612 - BWI76_RS00850 0.87 -2.7 176,652 + -0.3 176,655 - -1.5 176,669 + -2.7 176,669 + -4.1 176,737 + -0.0 176,861 + -3.1 176,876 + -2.5 176,917 + -3.0
Or see this region's nucleotide sequence