Experiment: MRS + kanamycin Time9hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16785 and BBR_RS16790 are separated by 233 nucleotides BBR_RS16790 and BBR_RS16795 are separated by 93 nucleotides BBR_RS16795 and BBR_RS16800 are separated by 40 nucleotides
BBR_RS16785: BBR_RS16785 - thiamine diphosphokinase, at 1,535,658 to 1,536,383
_RS16785
BBR_RS16790: BBR_RS16790 - type I glyceraldehyde-3-phosphate dehydrogenase, at 1,536,617 to 1,537,675
_RS16790
BBR_RS16795: BBR_RS16795 - Cys-tRNA(Pro) deacylase, at 1,537,769 to 1,538,275
_RS16795
BBR_RS16800: BBR_RS16800 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 1,538,316 to 1,539,308
_RS16800
Position (kb)
1536
1537
1538 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1535.624 kb on + strand at 1535.648 kb on - strand at 1535.649 kb on - strand at 1535.656 kb on - strand at 1536.524 kb on + strand at 1536.530 kb on - strand at 1537.677 kb on + strand at 1537.684 kb on + strand at 1537.748 kb on + strand at 1537.773 kb on - strand at 1537.904 kb on - strand, within BBR_RS16795 at 1537.915 kb on + strand, within BBR_RS16795 at 1537.931 kb on + strand, within BBR_RS16795 at 1537.988 kb on + strand, within BBR_RS16795 at 1538.046 kb on - strand, within BBR_RS16795 at 1538.155 kb on - strand, within BBR_RS16795 at 1538.203 kb on + strand, within BBR_RS16795 at 1538.211 kb on - strand, within BBR_RS16795 at 1538.230 kb on + strand at 1538.238 kb on - strand at 1538.306 kb on - strand at 1538.333 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time9hr remove 1,535,624 + -1.2 1,535,648 - +0.1 1,535,649 - +0.7 1,535,656 - -0.9 1,536,524 + -0.9 1,536,530 - +0.7 1,537,677 + -1.1 1,537,684 + +0.5 1,537,748 + -4.2 1,537,773 - +0.0 1,537,904 - BBR_RS16795 0.27 +2.7 1,537,915 + BBR_RS16795 0.29 +2.8 1,537,931 + BBR_RS16795 0.32 +2.4 1,537,988 + BBR_RS16795 0.43 +1.4 1,538,046 - BBR_RS16795 0.55 +1.1 1,538,155 - BBR_RS16795 0.76 -0.1 1,538,203 + BBR_RS16795 0.86 +1.0 1,538,211 - BBR_RS16795 0.87 -0.3 1,538,230 + +0.6 1,538,238 - +1.6 1,538,306 - +0.9 1,538,333 + +0.9
Or see this region's nucleotide sequence