Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS16790

Experiment: MRS + kanamycin Time9hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS16785 and BBR_RS16790 are separated by 233 nucleotidesBBR_RS16790 and BBR_RS16795 are separated by 93 nucleotidesBBR_RS16795 and BBR_RS16800 are separated by 40 nucleotides BBR_RS16785: BBR_RS16785 - thiamine diphosphokinase, at 1,535,658 to 1,536,383 _RS16785 BBR_RS16790: BBR_RS16790 - type I glyceraldehyde-3-phosphate dehydrogenase, at 1,536,617 to 1,537,675 _RS16790 BBR_RS16795: BBR_RS16795 - Cys-tRNA(Pro) deacylase, at 1,537,769 to 1,538,275 _RS16795 BBR_RS16800: BBR_RS16800 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 1,538,316 to 1,539,308 _RS16800 Position (kb) 1536 1537 1538Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1535.624 kb on + strandat 1535.648 kb on - strandat 1535.649 kb on - strandat 1535.656 kb on - strandat 1536.524 kb on + strandat 1536.530 kb on - strandat 1537.677 kb on + strandat 1537.684 kb on + strandat 1537.748 kb on + strandat 1537.773 kb on - strandat 1537.904 kb on - strand, within BBR_RS16795at 1537.915 kb on + strand, within BBR_RS16795at 1537.931 kb on + strand, within BBR_RS16795at 1537.988 kb on + strand, within BBR_RS16795at 1538.046 kb on - strand, within BBR_RS16795at 1538.155 kb on - strand, within BBR_RS16795at 1538.203 kb on + strand, within BBR_RS16795at 1538.211 kb on - strand, within BBR_RS16795at 1538.230 kb on + strandat 1538.238 kb on - strandat 1538.306 kb on - strandat 1538.333 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MRS + kanamycin Time9hr
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1,535,624 + -1.2
1,535,648 - +0.1
1,535,649 - +0.7
1,535,656 - -0.9
1,536,524 + -0.9
1,536,530 - +0.7
1,537,677 + -1.1
1,537,684 + +0.5
1,537,748 + -4.2
1,537,773 - +0.0
1,537,904 - BBR_RS16795 0.27 +2.7
1,537,915 + BBR_RS16795 0.29 +2.8
1,537,931 + BBR_RS16795 0.32 +2.4
1,537,988 + BBR_RS16795 0.43 +1.4
1,538,046 - BBR_RS16795 0.55 +1.1
1,538,155 - BBR_RS16795 0.76 -0.1
1,538,203 + BBR_RS16795 0.86 +1.0
1,538,211 - BBR_RS16795 0.87 -0.3
1,538,230 + +0.6
1,538,238 - +1.6
1,538,306 - +0.9
1,538,333 + +0.9

Or see this region's nucleotide sequence