Experiment: BG11 with Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livM and natD are separated by 3 nucleotides natD and livJ overlap by 1 nucleotides livJ and ndk are separated by 29 nucleotides ndk and Synpcc7942_2498 are separated by 99 nucleotides
Synpcc7942_2494: livM - putative branched-chain amino acid ABC transporter, permease protein, at 2,574,819 to 2,575,742
livM
Synpcc7942_2495: natD - integral membrane protein of the ABC-type Nat permease for neutral amino acids NatD, at 2,575,746 to 2,576,687
natD
Synpcc7942_2496: livJ - branched-chain amino-acid ABC transport system periplasmic binding protein, at 2,576,687 to 2,577,886
livJ
Synpcc7942_2497: ndk - nucleoside diphosphate kinase, at 2,577,916 to 2,578,371
ndk
Synpcc7942_2498: Synpcc7942_2498 - Band 7 protein, at 2,578,471 to 2,579,715
_2498
Position (kb)
2576
2577
2578 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2575.692 kb on - strand at 2575.716 kb on + strand at 2575.724 kb on - strand at 2575.732 kb on + strand at 2575.743 kb on + strand at 2575.743 kb on + strand at 2575.743 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.749 kb on + strand at 2575.751 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.767 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.786 kb on - strand at 2575.787 kb on - strand at 2575.799 kb on - strand at 2575.799 kb on - strand at 2575.799 kb on - strand at 2575.800 kb on - strand at 2575.804 kb on + strand at 2575.812 kb on - strand at 2575.821 kb on + strand at 2575.822 kb on - strand at 2575.846 kb on - strand, within natD at 2575.884 kb on - strand, within natD at 2575.897 kb on + strand, within natD at 2575.923 kb on + strand, within natD at 2575.923 kb on + strand, within natD at 2575.932 kb on - strand, within natD at 2575.937 kb on - strand, within natD at 2575.946 kb on + strand, within natD at 2575.991 kb on + strand, within natD at 2576.000 kb on + strand, within natD at 2576.092 kb on - strand, within natD at 2576.113 kb on + strand, within natD at 2576.129 kb on - strand, within natD at 2576.148 kb on - strand, within natD at 2576.149 kb on + strand, within natD at 2576.161 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.214 kb on - strand, within natD at 2576.214 kb on - strand, within natD at 2576.219 kb on - strand, within natD at 2576.219 kb on - strand, within natD at 2576.222 kb on + strand, within natD at 2576.230 kb on - strand, within natD at 2576.243 kb on - strand, within natD at 2576.249 kb on - strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.256 kb on - strand at 2576.258 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.263 kb on + strand, within natD at 2576.270 kb on - strand at 2576.271 kb on - strand, within natD at 2576.282 kb on - strand, within natD at 2576.282 kb on - strand, within natD at 2576.284 kb on + strand, within natD at 2576.357 kb on + strand, within natD at 2576.364 kb on - strand, within natD at 2576.364 kb on - strand, within natD at 2576.364 kb on - strand, within natD at 2576.392 kb on + strand, within natD at 2576.413 kb on + strand, within natD at 2576.413 kb on + strand, within natD at 2576.464 kb on - strand, within natD at 2576.476 kb on + strand, within natD at 2576.479 kb on - strand, within natD at 2576.484 kb on - strand, within natD at 2576.484 kb on - strand, within natD at 2576.486 kb on + strand, within natD at 2576.536 kb on + strand, within natD at 2576.544 kb on - strand, within natD at 2576.592 kb on + strand, within natD at 2576.613 kb on + strand at 2576.615 kb on - strand at 2576.621 kb on - strand at 2576.622 kb on - strand at 2576.641 kb on - strand at 2576.641 kb on - strand at 2576.641 kb on - strand at 2576.657 kb on + strand at 2576.657 kb on + strand at 2576.667 kb on - strand at 2576.698 kb on + strand at 2576.698 kb on + strand at 2576.707 kb on + strand at 2576.747 kb on + strand at 2576.750 kb on - strand at 2576.760 kb on - strand at 2576.775 kb on + strand at 2576.776 kb on + strand at 2576.776 kb on + strand at 2576.776 kb on + strand at 2576.776 kb on + strand at 2576.784 kb on - strand at 2576.792 kb on + strand at 2576.800 kb on - strand at 2576.800 kb on - strand at 2576.803 kb on + strand at 2576.803 kb on + strand at 2576.821 kb on + strand, within livJ at 2576.831 kb on + strand, within livJ at 2576.838 kb on + strand, within livJ at 2576.838 kb on + strand, within livJ at 2576.838 kb on + strand, within livJ at 2576.839 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.864 kb on + strand, within livJ at 2576.864 kb on + strand, within livJ at 2576.865 kb on - strand, within livJ at 2576.866 kb on - strand, within livJ at 2576.900 kb on - strand, within livJ at 2576.912 kb on + strand, within livJ at 2576.920 kb on - strand, within livJ at 2576.920 kb on - strand, within livJ at 2576.920 kb on - strand, within livJ at 2576.959 kb on - strand, within livJ at 2576.973 kb on + strand, within livJ at 2576.998 kb on - strand, within livJ at 2577.021 kb on + strand, within livJ at 2577.048 kb on + strand, within livJ at 2577.066 kb on - strand, within livJ at 2577.091 kb on + strand, within livJ at 2577.116 kb on + strand, within livJ at 2577.116 kb on + strand, within livJ at 2577.165 kb on + strand, within livJ at 2577.166 kb on + strand, within livJ at 2577.193 kb on - strand, within livJ at 2577.213 kb on - strand, within livJ at 2577.246 kb on + strand, within livJ at 2577.254 kb on - strand, within livJ at 2577.260 kb on - strand, within livJ at 2577.277 kb on - strand, within livJ at 2577.277 kb on - strand, within livJ at 2577.356 kb on - strand, within livJ at 2577.376 kb on + strand, within livJ at 2577.387 kb on + strand, within livJ at 2577.395 kb on + strand, within livJ at 2577.401 kb on + strand, within livJ at 2577.403 kb on - strand, within livJ at 2577.423 kb on + strand, within livJ at 2577.424 kb on - strand, within livJ at 2577.482 kb on + strand, within livJ at 2577.611 kb on + strand, within livJ at 2577.619 kb on - strand, within livJ at 2577.619 kb on - strand, within livJ at 2577.632 kb on - strand, within livJ at 2577.668 kb on - strand, within livJ at 2577.670 kb on + strand, within livJ at 2577.688 kb on - strand, within livJ at 2577.701 kb on + strand, within livJ at 2577.717 kb on - strand, within livJ at 2577.722 kb on - strand, within livJ at 2577.755 kb on + strand, within livJ at 2577.763 kb on - strand, within livJ at 2577.763 kb on - strand, within livJ at 2577.769 kb on - strand at 2577.791 kb on + strand at 2577.829 kb on - strand at 2577.834 kb on - strand at 2577.835 kb on + strand at 2577.835 kb on + strand at 2577.843 kb on - strand at 2577.848 kb on + strand at 2577.856 kb on - strand at 2577.858 kb on + strand at 2577.889 kb on - strand at 2577.907 kb on - strand at 2578.369 kb on + strand at 2578.369 kb on + strand at 2578.369 kb on + strand at 2578.369 kb on + strand at 2578.381 kb on + strand at 2578.382 kb on - strand at 2578.397 kb on + strand at 2578.405 kb on - strand at 2578.419 kb on - strand at 2578.438 kb on + strand at 2578.449 kb on + strand at 2578.467 kb on - strand at 2578.468 kb on + strand at 2578.468 kb on + strand at 2578.477 kb on - strand at 2578.477 kb on - strand at 2578.477 kb on - strand at 2578.495 kb on + strand at 2578.579 kb on + strand at 2578.579 kb on + strand at 2578.587 kb on - strand at 2578.601 kb on + strand, within Synpcc7942_2498 at 2578.621 kb on + strand, within Synpcc7942_2498 at 2578.631 kb on + strand, within Synpcc7942_2498 at 2578.631 kb on + strand, within Synpcc7942_2498 at 2578.639 kb on - strand, within Synpcc7942_2498 at 2578.682 kb on - strand, within Synpcc7942_2498 at 2578.712 kb on + strand, within Synpcc7942_2498 at 2578.777 kb on + strand, within Synpcc7942_2498 at 2578.801 kb on + strand, within Synpcc7942_2498 at 2578.811 kb on + strand, within Synpcc7942_2498 at 2578.815 kb on + strand, within Synpcc7942_2498 at 2578.833 kb on - strand, within Synpcc7942_2498 at 2578.834 kb on + strand, within Synpcc7942_2498 at 2578.835 kb on + strand, within Synpcc7942_2498 at 2578.870 kb on + strand, within Synpcc7942_2498 at 2578.878 kb on - strand, within Synpcc7942_2498 at 2578.883 kb on - strand, within Synpcc7942_2498
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Fusidic 0.5 mg/ml remove 2,575,692 - -0.6 2,575,716 + -0.9 2,575,724 - +1.1 2,575,732 + +0.3 2,575,743 + +2.3 2,575,743 + -0.6 2,575,743 + +0.1 2,575,744 + -0.7 2,575,744 + +2.9 2,575,744 + +0.4 2,575,744 + +0.6 2,575,749 + +1.0 2,575,751 - -0.5 2,575,752 - -0.2 2,575,752 - -0.5 2,575,752 - -0.9 2,575,752 - +0.1 2,575,767 - -1.3 2,575,776 - +0.4 2,575,776 - -0.2 2,575,776 - -0.5 2,575,776 - -0.0 2,575,786 - +1.5 2,575,787 - -1.2 2,575,799 - +0.7 2,575,799 - -0.4 2,575,799 - -1.0 2,575,800 - -1.0 2,575,804 + -0.2 2,575,812 - +0.3 2,575,821 + +0.0 2,575,822 - -0.3 2,575,846 - natD Synpcc7942_2495 0.11 -0.3 2,575,884 - natD Synpcc7942_2495 0.15 -0.4 2,575,897 + natD Synpcc7942_2495 0.16 -0.8 2,575,923 + natD Synpcc7942_2495 0.19 -0.0 2,575,923 + natD Synpcc7942_2495 0.19 +0.7 2,575,932 - natD Synpcc7942_2495 0.20 -1.2 2,575,937 - natD Synpcc7942_2495 0.20 +1.2 2,575,946 + natD Synpcc7942_2495 0.21 +0.3 2,575,991 + natD Synpcc7942_2495 0.26 -0.3 2,576,000 + natD Synpcc7942_2495 0.27 +0.5 2,576,092 - natD Synpcc7942_2495 0.37 -1.7 2,576,113 + natD Synpcc7942_2495 0.39 +1.3 2,576,129 - natD Synpcc7942_2495 0.41 -1.5 2,576,148 - natD Synpcc7942_2495 0.43 -0.3 2,576,149 + natD Synpcc7942_2495 0.43 +0.8 2,576,161 + natD Synpcc7942_2495 0.44 -0.2 2,576,196 + natD Synpcc7942_2495 0.48 -2.2 2,576,196 + natD Synpcc7942_2495 0.48 -0.8 2,576,196 + natD Synpcc7942_2495 0.48 +1.1 2,576,204 - natD Synpcc7942_2495 0.49 -0.5 2,576,204 - natD Synpcc7942_2495 0.49 -0.5 2,576,204 - natD Synpcc7942_2495 0.49 +0.7 2,576,204 - natD Synpcc7942_2495 0.49 +0.7 2,576,214 - natD Synpcc7942_2495 0.50 -0.9 2,576,214 - natD Synpcc7942_2495 0.50 +0.1 2,576,219 - natD Synpcc7942_2495 0.50 -0.9 2,576,219 - natD Synpcc7942_2495 0.50 -1.2 2,576,222 + natD Synpcc7942_2495 0.51 -0.3 2,576,230 - natD Synpcc7942_2495 0.51 -1.0 2,576,243 - natD Synpcc7942_2495 0.53 -1.2 2,576,249 - natD Synpcc7942_2495 0.53 +0.5 2,576,253 + natD Synpcc7942_2495 0.54 +0.5 2,576,253 + natD Synpcc7942_2495 0.54 -0.4 2,576,253 + natD Synpcc7942_2495 0.54 -0.7 2,576,253 + natD Synpcc7942_2495 0.54 +1.0 2,576,256 - +1.3 2,576,258 - natD Synpcc7942_2495 0.54 -2.0 2,576,261 - natD Synpcc7942_2495 0.55 +1.1 2,576,261 - natD Synpcc7942_2495 0.55 -2.2 2,576,261 - natD Synpcc7942_2495 0.55 +1.6 2,576,263 + natD Synpcc7942_2495 0.55 -0.7 2,576,270 - -0.3 2,576,271 - natD Synpcc7942_2495 0.56 +0.3 2,576,282 - natD Synpcc7942_2495 0.57 -2.2 2,576,282 - natD Synpcc7942_2495 0.57 +0.2 2,576,284 + natD Synpcc7942_2495 0.57 -0.7 2,576,357 + natD Synpcc7942_2495 0.65 +1.7 2,576,364 - natD Synpcc7942_2495 0.66 -1.8 2,576,364 - natD Synpcc7942_2495 0.66 +0.1 2,576,364 - natD Synpcc7942_2495 0.66 -1.3 2,576,392 + natD Synpcc7942_2495 0.69 +0.4 2,576,413 + natD Synpcc7942_2495 0.71 +0.1 2,576,413 + natD Synpcc7942_2495 0.71 -0.6 2,576,464 - natD Synpcc7942_2495 0.76 -0.8 2,576,476 + natD Synpcc7942_2495 0.77 -1.4 2,576,479 - natD Synpcc7942_2495 0.78 +1.0 2,576,484 - natD Synpcc7942_2495 0.78 +0.5 2,576,484 - natD Synpcc7942_2495 0.78 +0.2 2,576,486 + natD Synpcc7942_2495 0.79 -0.5 2,576,536 + natD Synpcc7942_2495 0.84 -0.2 2,576,544 - natD Synpcc7942_2495 0.85 -1.3 2,576,592 + natD Synpcc7942_2495 0.90 -0.2 2,576,613 + -1.6 2,576,615 - -1.3 2,576,621 - -1.2 2,576,622 - -0.3 2,576,641 - +1.0 2,576,641 - -2.0 2,576,641 - +0.0 2,576,657 + -2.0 2,576,657 + -0.6 2,576,667 - +0.0 2,576,698 + +0.2 2,576,698 + +0.3 2,576,707 + -0.0 2,576,747 + -1.4 2,576,750 - +0.5 2,576,760 - -0.1 2,576,775 + -0.3 2,576,776 + -1.0 2,576,776 + +0.3 2,576,776 + -0.2 2,576,776 + +1.3 2,576,784 - +0.2 2,576,792 + +0.2 2,576,800 - -1.0 2,576,800 - +1.2 2,576,803 + -2.0 2,576,803 + -0.2 2,576,821 + livJ Synpcc7942_2496 0.11 -0.7 2,576,831 + livJ Synpcc7942_2496 0.12 -0.4 2,576,838 + livJ Synpcc7942_2496 0.13 -1.7 2,576,838 + livJ Synpcc7942_2496 0.13 +0.3 2,576,838 + livJ Synpcc7942_2496 0.13 +1.1 2,576,839 - livJ Synpcc7942_2496 0.13 -0.8 2,576,846 - livJ Synpcc7942_2496 0.13 -0.3 2,576,846 - livJ Synpcc7942_2496 0.13 -0.3 2,576,846 - livJ Synpcc7942_2496 0.13 -0.1 2,576,846 - livJ Synpcc7942_2496 0.13 -1.5 2,576,846 - livJ Synpcc7942_2496 0.13 -2.9 2,576,864 + livJ Synpcc7942_2496 0.15 -0.4 2,576,864 + livJ Synpcc7942_2496 0.15 -0.6 2,576,865 - livJ Synpcc7942_2496 0.15 +0.1 2,576,866 - livJ Synpcc7942_2496 0.15 -0.4 2,576,900 - livJ Synpcc7942_2496 0.18 -0.0 2,576,912 + livJ Synpcc7942_2496 0.19 -0.0 2,576,920 - livJ Synpcc7942_2496 0.19 +0.7 2,576,920 - livJ Synpcc7942_2496 0.19 -0.9 2,576,920 - livJ Synpcc7942_2496 0.19 -0.2 2,576,959 - livJ Synpcc7942_2496 0.23 -0.5 2,576,973 + livJ Synpcc7942_2496 0.24 -0.5 2,576,998 - livJ Synpcc7942_2496 0.26 -0.2 2,577,021 + livJ Synpcc7942_2496 0.28 -0.3 2,577,048 + livJ Synpcc7942_2496 0.30 -1.3 2,577,066 - livJ Synpcc7942_2496 0.32 -0.7 2,577,091 + livJ Synpcc7942_2496 0.34 +0.7 2,577,116 + livJ Synpcc7942_2496 0.36 -0.3 2,577,116 + livJ Synpcc7942_2496 0.36 -2.0 2,577,165 + livJ Synpcc7942_2496 0.40 -0.7 2,577,166 + livJ Synpcc7942_2496 0.40 -0.6 2,577,193 - livJ Synpcc7942_2496 0.42 +1.4 2,577,213 - livJ Synpcc7942_2496 0.44 +0.8 2,577,246 + livJ Synpcc7942_2496 0.47 -0.6 2,577,254 - livJ Synpcc7942_2496 0.47 -0.6 2,577,260 - livJ Synpcc7942_2496 0.48 -0.4 2,577,277 - livJ Synpcc7942_2496 0.49 -0.8 2,577,277 - livJ Synpcc7942_2496 0.49 -0.1 2,577,356 - livJ Synpcc7942_2496 0.56 +0.2 2,577,376 + livJ Synpcc7942_2496 0.57 -1.7 2,577,387 + livJ Synpcc7942_2496 0.58 -0.7 2,577,395 + livJ Synpcc7942_2496 0.59 +1.3 2,577,401 + livJ Synpcc7942_2496 0.59 -0.3 2,577,403 - livJ Synpcc7942_2496 0.60 -0.4 2,577,423 + livJ Synpcc7942_2496 0.61 -0.6 2,577,424 - livJ Synpcc7942_2496 0.61 -0.4 2,577,482 + livJ Synpcc7942_2496 0.66 -0.5 2,577,611 + livJ Synpcc7942_2496 0.77 -0.8 2,577,619 - livJ Synpcc7942_2496 0.78 -0.0 2,577,619 - livJ Synpcc7942_2496 0.78 -0.9 2,577,632 - livJ Synpcc7942_2496 0.79 -0.2 2,577,668 - livJ Synpcc7942_2496 0.82 +0.0 2,577,670 + livJ Synpcc7942_2496 0.82 +1.5 2,577,688 - livJ Synpcc7942_2496 0.83 +0.1 2,577,701 + livJ Synpcc7942_2496 0.84 -0.9 2,577,717 - livJ Synpcc7942_2496 0.86 -1.4 2,577,722 - livJ Synpcc7942_2496 0.86 -0.8 2,577,755 + livJ Synpcc7942_2496 0.89 +0.3 2,577,763 - livJ Synpcc7942_2496 0.90 -1.6 2,577,763 - livJ Synpcc7942_2496 0.90 -0.6 2,577,769 - +0.6 2,577,791 + +0.1 2,577,829 - -2.5 2,577,834 - -0.4 2,577,835 + -0.7 2,577,835 + -1.6 2,577,843 - -0.2 2,577,848 + -0.7 2,577,856 - -1.2 2,577,858 + -0.4 2,577,889 - -0.0 2,577,907 - -0.0 2,578,369 + -1.7 2,578,369 + -0.5 2,578,369 + -1.1 2,578,369 + -1.1 2,578,381 + -0.7 2,578,382 - +1.8 2,578,397 + -0.0 2,578,405 - -1.2 2,578,419 - -1.1 2,578,438 + +1.1 2,578,449 + -0.7 2,578,467 - -0.7 2,578,468 + +1.0 2,578,468 + -0.3 2,578,477 - +0.3 2,578,477 - +0.2 2,578,477 - +0.0 2,578,495 + +1.3 2,578,579 + +1.0 2,578,579 + -0.3 2,578,587 - +0.1 2,578,601 + Synpcc7942_2498 0.10 +1.3 2,578,621 + Synpcc7942_2498 0.12 +0.7 2,578,631 + Synpcc7942_2498 0.13 +0.7 2,578,631 + Synpcc7942_2498 0.13 -1.5 2,578,639 - Synpcc7942_2498 0.13 -0.9 2,578,682 - Synpcc7942_2498 0.17 -1.0 2,578,712 + Synpcc7942_2498 0.19 +0.9 2,578,777 + Synpcc7942_2498 0.25 +0.3 2,578,801 + Synpcc7942_2498 0.27 +0.6 2,578,811 + Synpcc7942_2498 0.27 +2.3 2,578,815 + Synpcc7942_2498 0.28 +0.3 2,578,833 - Synpcc7942_2498 0.29 -0.5 2,578,834 + Synpcc7942_2498 0.29 +1.3 2,578,835 + Synpcc7942_2498 0.29 -0.4 2,578,870 + Synpcc7942_2498 0.32 -1.1 2,578,878 - Synpcc7942_2498 0.33 -0.8 2,578,883 - Synpcc7942_2498 0.33 -1.6
Or see this region's nucleotide sequence