Experiment: BG11 with Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0692 and Synpcc7942_0693 are separated by 61 nucleotides Synpcc7942_0693 and rps1 are separated by 52 nucleotides rps1 and Synpcc7942_0695 are separated by 277 nucleotides Synpcc7942_0695 and psbT are separated by 209 nucleotides
Synpcc7942_0692: Synpcc7942_0692 - hypothetical protein, at 685,631 to 686,185
_0692
Synpcc7942_0693: Synpcc7942_0693 - hypothetical protein, at 686,247 to 687,080
_0693
Synpcc7942_0694: rps1 - 30S ribosomal protein S1, at 687,133 to 688,056
rps1
Synpcc7942_0695: Synpcc7942_0695 - hypothetical protein, at 688,334 to 688,840
_0695
Synpcc7942_0696: psbT - photosystem II reaction center protein T, at 689,050 to 689,145
psbT
Position (kb) 687
688
689 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 686.138 kb on - strand at 686.138 kb on - strand at 686.139 kb on - strand at 686.140 kb on - strand at 686.164 kb on - strand at 686.168 kb on + strand at 686.189 kb on + strand at 686.189 kb on + strand at 686.189 kb on + strand at 686.196 kb on - strand at 686.197 kb on - strand at 686.203 kb on - strand at 686.214 kb on + strand at 686.224 kb on - strand at 686.254 kb on + strand at 686.345 kb on + strand, within Synpcc7942_0693 at 686.355 kb on + strand, within Synpcc7942_0693 at 686.449 kb on + strand, within Synpcc7942_0693 at 686.457 kb on - strand, within Synpcc7942_0693 at 686.469 kb on + strand, within Synpcc7942_0693 at 686.491 kb on + strand, within Synpcc7942_0693 at 686.576 kb on - strand, within Synpcc7942_0693 at 686.576 kb on - strand at 686.585 kb on + strand, within Synpcc7942_0693 at 686.669 kb on - strand, within Synpcc7942_0693 at 686.679 kb on - strand, within Synpcc7942_0693 at 686.728 kb on - strand, within Synpcc7942_0693 at 686.728 kb on - strand, within Synpcc7942_0693 at 686.739 kb on + strand, within Synpcc7942_0693 at 686.748 kb on - strand, within Synpcc7942_0693 at 686.753 kb on - strand, within Synpcc7942_0693 at 686.840 kb on + strand, within Synpcc7942_0693 at 686.852 kb on + strand, within Synpcc7942_0693 at 686.893 kb on + strand, within Synpcc7942_0693 at 686.904 kb on + strand, within Synpcc7942_0693 at 686.904 kb on + strand, within Synpcc7942_0693 at 686.912 kb on - strand, within Synpcc7942_0693 at 686.919 kb on + strand, within Synpcc7942_0693 at 686.935 kb on + strand, within Synpcc7942_0693 at 686.946 kb on + strand, within Synpcc7942_0693 at 686.947 kb on + strand, within Synpcc7942_0693 at 686.954 kb on - strand, within Synpcc7942_0693 at 686.955 kb on - strand, within Synpcc7942_0693 at 686.970 kb on + strand, within Synpcc7942_0693 at 686.970 kb on + strand, within Synpcc7942_0693 at 686.983 kb on - strand, within Synpcc7942_0693 at 686.998 kb on + strand at 686.998 kb on + strand at 687.033 kb on + strand at 687.038 kb on + strand at 687.046 kb on + strand at 687.054 kb on - strand at 687.063 kb on + strand at 687.148 kb on - strand at 688.236 kb on + strand at 688.365 kb on + strand at 688.386 kb on + strand, within Synpcc7942_0695 at 688.434 kb on + strand, within Synpcc7942_0695 at 688.442 kb on - strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand, within Synpcc7942_0695 at 688.552 kb on + strand at 688.552 kb on + strand, within Synpcc7942_0695 at 688.558 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.560 kb on - strand, within Synpcc7942_0695 at 688.571 kb on - strand, within Synpcc7942_0695 at 688.622 kb on + strand, within Synpcc7942_0695 at 688.627 kb on + strand, within Synpcc7942_0695 at 688.692 kb on - strand, within Synpcc7942_0695 at 688.722 kb on + strand, within Synpcc7942_0695 at 688.759 kb on - strand, within Synpcc7942_0695 at 688.760 kb on + strand, within Synpcc7942_0695 at 688.787 kb on - strand, within Synpcc7942_0695 at 688.788 kb on - strand, within Synpcc7942_0695 at 688.825 kb on + strand at 688.871 kb on + strand at 689.018 kb on - strand at 689.021 kb on + strand at 689.021 kb on + strand at 689.028 kb on + strand at 689.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Fusidic 0.5 mg/ml remove 686,138 - -1.2 686,138 - -0.7 686,139 - +0.8 686,140 - +0.9 686,164 - -0.7 686,168 + +0.3 686,189 + +0.5 686,189 + -0.6 686,189 + -0.2 686,196 - -0.3 686,197 - -0.2 686,203 - -0.8 686,214 + +0.5 686,224 - -0.3 686,254 + -0.2 686,345 + Synpcc7942_0693 0.12 +3.7 686,355 + Synpcc7942_0693 0.13 +3.2 686,449 + Synpcc7942_0693 0.24 +3.3 686,457 - Synpcc7942_0693 0.25 +3.8 686,469 + Synpcc7942_0693 0.27 +5.3 686,491 + Synpcc7942_0693 0.29 +4.1 686,576 - Synpcc7942_0693 0.39 +2.2 686,576 - +5.7 686,585 + Synpcc7942_0693 0.41 +6.3 686,669 - Synpcc7942_0693 0.51 +4.2 686,679 - Synpcc7942_0693 0.52 +4.0 686,728 - Synpcc7942_0693 0.58 +2.0 686,728 - Synpcc7942_0693 0.58 +2.6 686,739 + Synpcc7942_0693 0.59 +2.1 686,748 - Synpcc7942_0693 0.60 +5.0 686,753 - Synpcc7942_0693 0.61 +4.1 686,840 + Synpcc7942_0693 0.71 +3.9 686,852 + Synpcc7942_0693 0.73 +5.6 686,893 + Synpcc7942_0693 0.77 +4.6 686,904 + Synpcc7942_0693 0.79 +4.7 686,904 + Synpcc7942_0693 0.79 +3.2 686,912 - Synpcc7942_0693 0.80 +3.8 686,919 + Synpcc7942_0693 0.81 +4.6 686,935 + Synpcc7942_0693 0.82 +4.2 686,946 + Synpcc7942_0693 0.84 +3.2 686,947 + Synpcc7942_0693 0.84 +4.2 686,954 - Synpcc7942_0693 0.85 +3.0 686,955 - Synpcc7942_0693 0.85 +4.6 686,970 + Synpcc7942_0693 0.87 +3.7 686,970 + Synpcc7942_0693 0.87 +4.2 686,983 - Synpcc7942_0693 0.88 +2.8 686,998 + -1.0 686,998 + -1.0 687,033 + +1.0 687,038 + +0.1 687,046 + -0.7 687,054 - +0.9 687,063 + +0.3 687,148 - -0.8 688,236 + -1.1 688,365 + -1.0 688,386 + Synpcc7942_0695 0.10 -1.6 688,434 + Synpcc7942_0695 0.20 +0.1 688,442 - Synpcc7942_0695 0.21 +0.8 688,552 + Synpcc7942_0695 0.43 -2.0 688,552 + Synpcc7942_0695 0.43 -3.2 688,552 + Synpcc7942_0695 0.43 -2.0 688,552 + Synpcc7942_0695 0.43 -2.3 688,552 + Synpcc7942_0695 0.43 -0.7 688,552 + Synpcc7942_0695 0.43 -1.3 688,552 + Synpcc7942_0695 0.43 -0.2 688,552 + Synpcc7942_0695 0.43 -0.4 688,552 + Synpcc7942_0695 0.43 -0.2 688,552 + Synpcc7942_0695 0.43 -1.2 688,552 + +0.0 688,552 + Synpcc7942_0695 0.43 -2.4 688,558 - Synpcc7942_0695 0.44 +0.3 688,560 - Synpcc7942_0695 0.45 +0.0 688,560 - Synpcc7942_0695 0.45 -0.5 688,560 - Synpcc7942_0695 0.45 -1.4 688,560 - Synpcc7942_0695 0.45 -0.8 688,560 - Synpcc7942_0695 0.45 -0.7 688,560 - Synpcc7942_0695 0.45 -1.7 688,560 - Synpcc7942_0695 0.45 +0.0 688,560 - Synpcc7942_0695 0.45 -0.8 688,560 - Synpcc7942_0695 0.45 -0.8 688,571 - Synpcc7942_0695 0.47 +0.2 688,622 + Synpcc7942_0695 0.57 +2.3 688,627 + Synpcc7942_0695 0.58 -1.0 688,692 - Synpcc7942_0695 0.71 -0.5 688,722 + Synpcc7942_0695 0.77 +0.8 688,759 - Synpcc7942_0695 0.84 -1.7 688,760 + Synpcc7942_0695 0.84 +0.3 688,787 - Synpcc7942_0695 0.89 +0.3 688,788 - Synpcc7942_0695 0.90 +0.0 688,825 + +1.3 688,871 + +0.8 689,018 - -0.8 689,021 + +2.3 689,021 + +1.0 689,028 + +2.1 689,029 - -1.0
Or see this region's nucleotide sequence