Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23630 and HSERO_RS23635 are separated by 68 nucleotides HSERO_RS23635 and HSERO_RS23640 are separated by 58 nucleotides HSERO_RS23640 and HSERO_RS23645 overlap by 19 nucleotides
HSERO_RS23630: HSERO_RS23630 - urease accessory protein UreF, at 5,417,990 to 5,418,670
_RS23630
HSERO_RS23635: HSERO_RS23635 - urease accessory protein UreG, at 5,418,739 to 5,419,371
_RS23635
HSERO_RS23640: HSERO_RS23640 - Ni/Fe hydrogenase, at 5,419,430 to 5,420,041
_RS23640
HSERO_RS23645: HSERO_RS23645 - glycerol-3-phosphate dehydrogenase, at 5,420,023 to 5,421,627
_RS23645
Position (kb) 5419
5420
5421 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5418.878 kb on - strand, within HSERO_RS23635 at 5419.035 kb on + strand, within HSERO_RS23635 at 5419.085 kb on + strand, within HSERO_RS23635 at 5419.293 kb on + strand, within HSERO_RS23635 at 5419.626 kb on - strand, within HSERO_RS23640 at 5419.636 kb on - strand, within HSERO_RS23640 at 5419.699 kb on - strand, within HSERO_RS23640 at 5419.845 kb on - strand, within HSERO_RS23640 at 5419.865 kb on - strand, within HSERO_RS23640 at 5419.998 kb on - strand at 5420.027 kb on - strand at 5420.321 kb on + strand, within HSERO_RS23645 at 5420.823 kb on - strand, within HSERO_RS23645 at 5420.882 kb on + strand, within HSERO_RS23645 at 5421.021 kb on - strand, within HSERO_RS23645 at 5421.033 kb on - strand, within HSERO_RS23645
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 5,418,878 - HSERO_RS23635 0.22 +0.8 5,419,035 + HSERO_RS23635 0.47 -0.2 5,419,085 + HSERO_RS23635 0.55 +0.9 5,419,293 + HSERO_RS23635 0.88 +0.0 5,419,626 - HSERO_RS23640 0.32 -1.2 5,419,636 - HSERO_RS23640 0.34 +3.5 5,419,699 - HSERO_RS23640 0.44 -1.1 5,419,845 - HSERO_RS23640 0.68 -2.6 5,419,865 - HSERO_RS23640 0.71 -0.5 5,419,998 - -2.6 5,420,027 - -1.4 5,420,321 + HSERO_RS23645 0.19 -0.5 5,420,823 - HSERO_RS23645 0.50 +0.2 5,420,882 + HSERO_RS23645 0.54 +1.1 5,421,021 - HSERO_RS23645 0.62 +0.0 5,421,033 - HSERO_RS23645 0.63 -0.2
Or see this region's nucleotide sequence