Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23465 and HSERO_RS23470 are separated by 85 nucleotides HSERO_RS23470 and HSERO_RS23475 are separated by 56 nucleotides HSERO_RS23475 and HSERO_RS23480 are separated by 131 nucleotides HSERO_RS23480 and HSERO_RS23485 are separated by 259 nucleotides
HSERO_RS23465: HSERO_RS23465 - beta-lactamase, at 5,382,992 to 5,383,921
_RS23465
HSERO_RS23470: HSERO_RS23470 - prolyl-tRNA synthetase, at 5,384,007 to 5,384,516
_RS23470
HSERO_RS23475: HSERO_RS23475 - hydroxymethylglutaryl-CoA lyase, at 5,384,573 to 5,385,481
_RS23475
HSERO_RS23480: HSERO_RS23480 - hypothetical protein, at 5,385,613 to 5,385,825
_RS23480
HSERO_RS23485: HSERO_RS23485 - 2-nitropropane dioxygenase, at 5,386,085 to 5,387,059
_RS23485
Position (kb) 5384
5385
5386 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5383.574 kb on - strand, within HSERO_RS23465 at 5383.724 kb on - strand, within HSERO_RS23465 at 5383.818 kb on + strand, within HSERO_RS23465 at 5383.865 kb on - strand at 5383.876 kb on + strand at 5383.884 kb on - strand at 5384.081 kb on - strand, within HSERO_RS23470 at 5384.099 kb on + strand, within HSERO_RS23470 at 5384.107 kb on - strand, within HSERO_RS23470 at 5384.107 kb on - strand, within HSERO_RS23470 at 5384.143 kb on + strand, within HSERO_RS23470 at 5384.244 kb on + strand, within HSERO_RS23470 at 5384.393 kb on - strand, within HSERO_RS23470 at 5384.437 kb on - strand, within HSERO_RS23470 at 5384.664 kb on - strand, within HSERO_RS23475 at 5384.795 kb on - strand, within HSERO_RS23475 at 5385.069 kb on + strand, within HSERO_RS23475 at 5385.081 kb on + strand, within HSERO_RS23475 at 5385.081 kb on + strand, within HSERO_RS23475 at 5385.223 kb on - strand, within HSERO_RS23475 at 5385.250 kb on + strand, within HSERO_RS23475 at 5385.476 kb on + strand at 5385.611 kb on + strand at 5386.113 kb on - strand at 5386.129 kb on - strand at 5386.141 kb on - strand at 5386.148 kb on + strand at 5386.189 kb on + strand, within HSERO_RS23485 at 5386.189 kb on + strand, within HSERO_RS23485 at 5386.199 kb on + strand, within HSERO_RS23485 at 5386.251 kb on + strand, within HSERO_RS23485 at 5386.309 kb on + strand, within HSERO_RS23485
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 5,383,574 - HSERO_RS23465 0.63 -0.2 5,383,724 - HSERO_RS23465 0.79 +0.2 5,383,818 + HSERO_RS23465 0.89 +1.4 5,383,865 - +1.2 5,383,876 + -0.6 5,383,884 - +0.2 5,384,081 - HSERO_RS23470 0.15 -1.0 5,384,099 + HSERO_RS23470 0.18 +0.5 5,384,107 - HSERO_RS23470 0.20 +0.3 5,384,107 - HSERO_RS23470 0.20 +0.2 5,384,143 + HSERO_RS23470 0.27 +0.1 5,384,244 + HSERO_RS23470 0.46 -0.8 5,384,393 - HSERO_RS23470 0.76 +0.4 5,384,437 - HSERO_RS23470 0.84 +0.4 5,384,664 - HSERO_RS23475 0.10 -1.4 5,384,795 - HSERO_RS23475 0.24 +0.5 5,385,069 + HSERO_RS23475 0.55 +0.3 5,385,081 + HSERO_RS23475 0.56 -1.2 5,385,081 + HSERO_RS23475 0.56 -0.4 5,385,223 - HSERO_RS23475 0.72 -0.4 5,385,250 + HSERO_RS23475 0.74 -0.9 5,385,476 + +0.1 5,385,611 + +1.9 5,386,113 - -1.3 5,386,129 - -0.1 5,386,141 - -0.6 5,386,148 + -1.8 5,386,189 + HSERO_RS23485 0.11 +0.3 5,386,189 + HSERO_RS23485 0.11 +3.6 5,386,199 + HSERO_RS23485 0.12 -1.0 5,386,251 + HSERO_RS23485 0.17 -0.6 5,386,309 + HSERO_RS23485 0.23 -0.3
Or see this region's nucleotide sequence