Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS20235

Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS20225 and HSERO_RS20230 are separated by 132 nucleotidesHSERO_RS20230 and HSERO_RS20235 are separated by 98 nucleotidesHSERO_RS20235 and HSERO_RS20240 are separated by 23 nucleotides HSERO_RS20225: HSERO_RS20225 - starvation protein B, at 4,642,298 to 4,642,795 _RS20225 HSERO_RS20230: HSERO_RS20230 - glutathione S-transferase, at 4,642,928 to 4,643,539 _RS20230 HSERO_RS20235: HSERO_RS20235 - cytochrome C, at 4,643,638 to 4,644,402 _RS20235 HSERO_RS20240: HSERO_RS20240 - cytochrome B, at 4,644,426 to 4,645,832 _RS20240 Position (kb) 4643 4644 4645Strain fitness (log2 ratio) -1 0 1at 4642.767 kb on + strandat 4642.775 kb on - strandat 4643.627 kb on - strandat 4643.651 kb on + strandat 4643.657 kb on - strandat 4643.685 kb on - strandat 4643.822 kb on + strand, within HSERO_RS20235at 4643.830 kb on - strand, within HSERO_RS20235at 4644.411 kb on - strandat 4644.487 kb on - strandat 4644.898 kb on - strand, within HSERO_RS20240at 4645.066 kb on + strand, within HSERO_RS20240

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
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4,642,767 + -0.3
4,642,775 - +0.2
4,643,627 - +0.8
4,643,651 + +0.4
4,643,657 - -0.7
4,643,685 - -1.4
4,643,822 + HSERO_RS20235 0.24 +1.0
4,643,830 - HSERO_RS20235 0.25 -1.7
4,644,411 - -0.6
4,644,487 - -1.1
4,644,898 - HSERO_RS20240 0.34 -0.4
4,645,066 + HSERO_RS20240 0.45 -0.4

Or see this region's nucleotide sequence