Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20025 and HSERO_RS20030 are separated by 13 nucleotides HSERO_RS20030 and HSERO_RS20035 are separated by 27 nucleotides HSERO_RS20035 and HSERO_RS20040 are separated by 16 nucleotides
HSERO_RS20025: HSERO_RS20025 - feruloyl-CoA synthase, at 4,595,210 to 4,597,072
_RS20025
HSERO_RS20030: HSERO_RS20030 - acetyl-CoA acetyltransferase, at 4,597,086 to 4,598,339
_RS20030
HSERO_RS20035: HSERO_RS20035 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 4,598,367 to 4,599,131
_RS20035
HSERO_RS20040: HSERO_RS20040 - 4-hydroxybenzoyl-CoA thioesterase, at 4,599,148 to 4,599,567
_RS20040
Position (kb) 4597
4598
4599 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4596.234 kb on - strand, within HSERO_RS20025 at 4596.250 kb on - strand, within HSERO_RS20025 at 4596.308 kb on + strand, within HSERO_RS20025 at 4596.316 kb on - strand, within HSERO_RS20025 at 4596.563 kb on + strand, within HSERO_RS20025 at 4596.608 kb on + strand, within HSERO_RS20025 at 4596.837 kb on + strand, within HSERO_RS20025 at 4596.968 kb on - strand at 4597.000 kb on + strand at 4597.003 kb on - strand at 4597.054 kb on + strand at 4597.075 kb on - strand at 4597.119 kb on - strand at 4597.121 kb on + strand at 4597.178 kb on - strand at 4597.245 kb on - strand, within HSERO_RS20030 at 4597.437 kb on - strand, within HSERO_RS20030 at 4597.517 kb on + strand, within HSERO_RS20030 at 4597.866 kb on + strand at 4597.912 kb on + strand, within HSERO_RS20030 at 4598.171 kb on - strand, within HSERO_RS20030 at 4598.356 kb on + strand at 4598.371 kb on - strand at 4598.415 kb on - strand at 4598.782 kb on - strand, within HSERO_RS20035 at 4598.904 kb on - strand at 4599.007 kb on - strand, within HSERO_RS20035 at 4599.162 kb on + strand at 4599.244 kb on - strand, within HSERO_RS20040
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 4,596,234 - HSERO_RS20025 0.55 +0.0 4,596,250 - HSERO_RS20025 0.56 -3.5 4,596,308 + HSERO_RS20025 0.59 +0.0 4,596,316 - HSERO_RS20025 0.59 +0.3 4,596,563 + HSERO_RS20025 0.73 +0.7 4,596,608 + HSERO_RS20025 0.75 +0.2 4,596,837 + HSERO_RS20025 0.87 +1.4 4,596,968 - +0.1 4,597,000 + +0.4 4,597,003 - +2.1 4,597,054 + -0.6 4,597,075 - -0.9 4,597,119 - +0.3 4,597,121 + +0.7 4,597,178 - -0.1 4,597,245 - HSERO_RS20030 0.13 +0.0 4,597,437 - HSERO_RS20030 0.28 +0.4 4,597,517 + HSERO_RS20030 0.34 +0.2 4,597,866 + +1.5 4,597,912 + HSERO_RS20030 0.66 +0.4 4,598,171 - HSERO_RS20030 0.87 +1.3 4,598,356 + +0.7 4,598,371 - +1.4 4,598,415 - -0.2 4,598,782 - HSERO_RS20035 0.54 +0.5 4,598,904 - -0.4 4,599,007 - HSERO_RS20035 0.84 +0.2 4,599,162 + -0.1 4,599,244 - HSERO_RS20040 0.23 -0.1
Or see this region's nucleotide sequence