Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS16065 and HSERO_RS16070 are separated by 2 nucleotides HSERO_RS16070 and HSERO_RS16075 are separated by 12 nucleotides HSERO_RS16075 and HSERO_RS16080 are separated by 453 nucleotides
HSERO_RS16065: HSERO_RS16065 - cation transporter, at 3,666,315 to 3,668,834
_RS16065
HSERO_RS16070: HSERO_RS16070 - cytochrome biogenesis protein, at 3,668,837 to 3,669,691
_RS16070
HSERO_RS16075: HSERO_RS16075 - coproporphyrinogen III oxidase, at 3,669,704 to 3,671,155
_RS16075
HSERO_RS16080: HSERO_RS16080 - histidine kinase, at 3,671,609 to 3,672,160
_RS16080
HSERO_RS16085: HSERO_RS16085 - aromatic amino acid exporter, at 3,672,148 to 3,673,071
_RS16085
Position (kb)
3669
3670
3671
3672 Strain fitness (log2 ratio)
-2
-1
0
1 at 3668.717 kb on + strand at 3669.011 kb on - strand, within HSERO_RS16070 at 3669.355 kb on + strand, within HSERO_RS16070 at 3669.363 kb on - strand, within HSERO_RS16070 at 3669.982 kb on + strand, within HSERO_RS16075 at 3670.361 kb on - strand, within HSERO_RS16075 at 3670.382 kb on + strand, within HSERO_RS16075 at 3670.472 kb on + strand, within HSERO_RS16075 at 3670.595 kb on + strand, within HSERO_RS16075 at 3671.074 kb on + strand at 3671.094 kb on + strand at 3671.169 kb on - strand at 3671.524 kb on + strand at 3671.711 kb on - strand, within HSERO_RS16080 at 3671.763 kb on - strand at 3671.845 kb on + strand, within HSERO_RS16080 at 3671.924 kb on - strand, within HSERO_RS16080 at 3671.928 kb on - strand, within HSERO_RS16080 at 3671.932 kb on + strand at 3671.935 kb on - strand, within HSERO_RS16080 at 3671.964 kb on - strand, within HSERO_RS16080 at 3672.041 kb on + strand, within HSERO_RS16080 at 3672.135 kb on + strand at 3672.140 kb on + strand at 3672.147 kb on - strand at 3672.154 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 3,668,717 + -1.6 3,669,011 - HSERO_RS16070 0.20 -2.7 3,669,355 + HSERO_RS16070 0.61 +0.5 3,669,363 - HSERO_RS16070 0.62 +0.2 3,669,982 + HSERO_RS16075 0.19 -0.2 3,670,361 - HSERO_RS16075 0.45 -2.2 3,670,382 + HSERO_RS16075 0.47 +0.5 3,670,472 + HSERO_RS16075 0.53 -0.7 3,670,595 + HSERO_RS16075 0.61 +0.1 3,671,074 + -0.5 3,671,094 + -0.2 3,671,169 - +1.1 3,671,524 + -0.2 3,671,711 - HSERO_RS16080 0.18 +1.2 3,671,763 - -1.2 3,671,845 + HSERO_RS16080 0.43 +0.5 3,671,924 - HSERO_RS16080 0.57 +0.9 3,671,928 - HSERO_RS16080 0.58 +1.1 3,671,932 + -0.5 3,671,935 - HSERO_RS16080 0.59 -1.9 3,671,964 - HSERO_RS16080 0.64 -1.5 3,672,041 + HSERO_RS16080 0.78 -0.2 3,672,135 + +0.2 3,672,140 + +0.3 3,672,147 - -0.7 3,672,154 + -0.4
Or see this region's nucleotide sequence