Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS15035 and HSERO_RS15040 are separated by 248 nucleotides HSERO_RS15040 and HSERO_RS15045 are separated by 57 nucleotides HSERO_RS15045 and HSERO_RS15050 are separated by 152 nucleotides HSERO_RS15050 and HSERO_RS15055 overlap by 4 nucleotides
HSERO_RS15035: HSERO_RS15035 - 3-ketoacyl-ACP reductase, at 3,412,873 to 3,413,613
_RS15035
HSERO_RS15040: HSERO_RS15040 - poly(R)-hydroxyalkanoic acid synthase, at 3,413,862 to 3,415,685
_RS15040
HSERO_RS15045: HSERO_RS15045 - membrane protein, at 3,415,743 to 3,416,012
_RS15045
HSERO_RS15050: HSERO_RS15050 - hypothetical protein, at 3,416,165 to 3,416,425
_RS15050
HSERO_RS15055: HSERO_RS15055 - membrane protein, at 3,416,422 to 3,417,204
_RS15055
Position (kb)
3413
3414
3415
3416 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3413.536 kb on - strand, within HSERO_RS15035 at 3413.611 kb on + strand at 3413.647 kb on + strand at 3413.655 kb on - strand at 3413.671 kb on + strand at 3413.718 kb on + strand at 3413.889 kb on - strand at 3413.999 kb on + strand at 3414.270 kb on - strand, within HSERO_RS15040 at 3414.617 kb on + strand, within HSERO_RS15040 at 3414.690 kb on - strand, within HSERO_RS15040 at 3414.949 kb on + strand, within HSERO_RS15040 at 3414.957 kb on - strand, within HSERO_RS15040 at 3414.957 kb on - strand, within HSERO_RS15040 at 3415.105 kb on + strand, within HSERO_RS15040 at 3415.252 kb on + strand, within HSERO_RS15040 at 3415.322 kb on - strand, within HSERO_RS15040 at 3415.330 kb on - strand, within HSERO_RS15040 at 3415.422 kb on - strand, within HSERO_RS15040 at 3415.422 kb on - strand, within HSERO_RS15040 at 3415.549 kb on + strand at 3415.604 kb on - strand at 3415.606 kb on + strand at 3415.606 kb on + strand at 3415.718 kb on - strand at 3415.802 kb on + strand, within HSERO_RS15045 at 3415.809 kb on + strand, within HSERO_RS15045 at 3415.843 kb on - strand, within HSERO_RS15045 at 3415.907 kb on - strand, within HSERO_RS15045 at 3416.011 kb on + strand at 3416.023 kb on + strand at 3416.033 kb on + strand at 3416.041 kb on - strand at 3416.343 kb on - strand, within HSERO_RS15050 at 3416.351 kb on + strand, within HSERO_RS15050 at 3416.353 kb on - strand, within HSERO_RS15050 at 3416.359 kb on - strand, within HSERO_RS15050 at 3416.585 kb on + strand, within HSERO_RS15055
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 3,413,536 - HSERO_RS15035 0.89 -2.3 3,413,611 + -0.8 3,413,647 + -0.2 3,413,655 - -0.1 3,413,671 + +1.1 3,413,718 + +0.7 3,413,889 - -0.4 3,413,999 + -2.6 3,414,270 - HSERO_RS15040 0.22 +2.1 3,414,617 + HSERO_RS15040 0.41 -1.0 3,414,690 - HSERO_RS15040 0.45 -0.7 3,414,949 + HSERO_RS15040 0.60 -1.7 3,414,957 - HSERO_RS15040 0.60 -1.1 3,414,957 - HSERO_RS15040 0.60 -0.2 3,415,105 + HSERO_RS15040 0.68 -1.5 3,415,252 + HSERO_RS15040 0.76 +0.1 3,415,322 - HSERO_RS15040 0.80 -0.7 3,415,330 - HSERO_RS15040 0.80 +0.5 3,415,422 - HSERO_RS15040 0.86 -0.9 3,415,422 - HSERO_RS15040 0.86 -1.8 3,415,549 + -0.1 3,415,604 - -0.8 3,415,606 + -1.8 3,415,606 + -1.0 3,415,718 - -1.2 3,415,802 + HSERO_RS15045 0.22 +0.3 3,415,809 + HSERO_RS15045 0.24 -1.1 3,415,843 - HSERO_RS15045 0.37 -0.5 3,415,907 - HSERO_RS15045 0.61 -0.1 3,416,011 + -0.7 3,416,023 + -0.4 3,416,033 + +0.2 3,416,041 - -1.1 3,416,343 - HSERO_RS15050 0.68 +0.9 3,416,351 + HSERO_RS15050 0.71 +1.8 3,416,353 - HSERO_RS15050 0.72 +0.5 3,416,359 - HSERO_RS15050 0.74 -0.0 3,416,585 + HSERO_RS15055 0.21 +1.4
Or see this region's nucleotide sequence