Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14255 and HSERO_RS14260 are separated by 59 nucleotides HSERO_RS14260 and HSERO_RS14265 overlap by 4 nucleotides HSERO_RS14265 and HSERO_RS14270 are separated by 26 nucleotides HSERO_RS14270 and HSERO_RS14275 are separated by 3 nucleotides HSERO_RS14275 and HSERO_RS14280 overlap by 4 nucleotides
HSERO_RS14255: HSERO_RS14255 - molybdenum processing protein, at 3,238,093 to 3,238,692
_RS14255
HSERO_RS14260: HSERO_RS14260 - ferredoxin, at 3,238,752 to 3,239,057
_RS14260
HSERO_RS14265: HSERO_RS14265 - hypothetical protein, at 3,239,054 to 3,239,260
_RS14265
HSERO_RS14270: HSERO_RS14270 - hypothetical protein, at 3,239,287 to 3,239,757
_RS14270
HSERO_RS14275: HSERO_RS14275 - nitrogen fixation protein NifX, at 3,239,761 to 3,240,162
_RS14275
HSERO_RS14280: HSERO_RS14280 - nitrogenase molybdenum-cofactor synthesis protein NifN protein, at 3,240,159 to 3,241,547
_RS14280
Position (kb) 3239
3240 Strain fitness (log2 ratio) -1
0
1
2 at 3238.296 kb on - strand, within HSERO_RS14255 at 3238.320 kb on - strand, within HSERO_RS14255 at 3238.390 kb on + strand, within HSERO_RS14255 at 3238.473 kb on + strand, within HSERO_RS14255 at 3238.495 kb on - strand, within HSERO_RS14255 at 3238.495 kb on - strand, within HSERO_RS14255 at 3238.643 kb on - strand at 3238.774 kb on + strand at 3238.907 kb on - strand, within HSERO_RS14260 at 3238.926 kb on - strand, within HSERO_RS14260 at 3239.294 kb on + strand at 3239.388 kb on - strand, within HSERO_RS14270 at 3239.534 kb on + strand, within HSERO_RS14270 at 3239.617 kb on + strand, within HSERO_RS14270 at 3239.772 kb on - strand at 3239.818 kb on - strand, within HSERO_RS14275 at 3239.818 kb on - strand, within HSERO_RS14275 at 3239.834 kb on - strand at 3240.181 kb on + strand at 3240.199 kb on - strand at 3240.307 kb on - strand, within HSERO_RS14280 at 3240.435 kb on - strand, within HSERO_RS14280 at 3240.457 kb on - strand, within HSERO_RS14280 at 3240.508 kb on + strand, within HSERO_RS14280 at 3240.527 kb on + strand, within HSERO_RS14280 at 3240.600 kb on + strand, within HSERO_RS14280 at 3240.745 kb on + strand, within HSERO_RS14280 at 3240.756 kb on + strand, within HSERO_RS14280
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 3,238,296 - HSERO_RS14255 0.34 +0.7 3,238,320 - HSERO_RS14255 0.38 +0.4 3,238,390 + HSERO_RS14255 0.49 -0.1 3,238,473 + HSERO_RS14255 0.63 +0.0 3,238,495 - HSERO_RS14255 0.67 -0.3 3,238,495 - HSERO_RS14255 0.67 -0.2 3,238,643 - +0.5 3,238,774 + -0.9 3,238,907 - HSERO_RS14260 0.51 -0.6 3,238,926 - HSERO_RS14260 0.57 -1.2 3,239,294 + +1.8 3,239,388 - HSERO_RS14270 0.21 +1.2 3,239,534 + HSERO_RS14270 0.52 -0.9 3,239,617 + HSERO_RS14270 0.70 +2.7 3,239,772 - +0.6 3,239,818 - HSERO_RS14275 0.14 +1.9 3,239,818 - HSERO_RS14275 0.14 +0.6 3,239,834 - -0.1 3,240,181 + +0.0 3,240,199 - -0.1 3,240,307 - HSERO_RS14280 0.11 +0.9 3,240,435 - HSERO_RS14280 0.20 +0.3 3,240,457 - HSERO_RS14280 0.21 +1.0 3,240,508 + HSERO_RS14280 0.25 -1.6 3,240,527 + HSERO_RS14280 0.26 +0.0 3,240,600 + HSERO_RS14280 0.32 +0.2 3,240,745 + HSERO_RS14280 0.42 -0.2 3,240,756 + HSERO_RS14280 0.43 +1.1
Or see this region's nucleotide sequence