Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13270 and HSERO_RS13275 are separated by 143 nucleotides HSERO_RS13275 and HSERO_RS13280 overlap by 4 nucleotides HSERO_RS13280 and HSERO_RS13285 overlap by 4 nucleotides HSERO_RS13285 and HSERO_RS13290 are separated by 8 nucleotides
HSERO_RS13270: HSERO_RS13270 - bifunctional precorrin-3 methyltransferase/precorrin-6x reductase oxidoreductase, at 3,023,458 to 3,025,017
_RS13270
HSERO_RS13275: HSERO_RS13275 - cobalamin biosynthesis protein CbiG, at 3,025,161 to 3,025,964
_RS13275
HSERO_RS13280: HSERO_RS13280 - precorrin-4 C11-methyltransferase, at 3,025,961 to 3,026,737
_RS13280
HSERO_RS13285: HSERO_RS13285 - precorrin-2 C20-methyltransferase, at 3,026,734 to 3,027,492
_RS13285
HSERO_RS13290: HSERO_RS13290 - cobalt-precorrin-6A synthase, at 3,027,501 to 3,028,691
_RS13290
Position (kb)
3025
3026
3027 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3024.974 kb on + strand at 3025.150 kb on - strand at 3025.397 kb on - strand, within HSERO_RS13275 at 3025.834 kb on - strand, within HSERO_RS13275 at 3025.931 kb on + strand at 3026.019 kb on - strand at 3026.078 kb on + strand, within HSERO_RS13280 at 3026.092 kb on - strand, within HSERO_RS13280 at 3026.461 kb on - strand, within HSERO_RS13280 at 3026.524 kb on + strand, within HSERO_RS13280 at 3027.007 kb on + strand, within HSERO_RS13285 at 3027.410 kb on - strand, within HSERO_RS13285 at 3027.565 kb on - strand at 3027.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 3,024,974 + -0.0 3,025,150 - +0.8 3,025,397 - HSERO_RS13275 0.29 -1.6 3,025,834 - HSERO_RS13275 0.84 -1.8 3,025,931 + -0.9 3,026,019 - -2.8 3,026,078 + HSERO_RS13280 0.15 -1.0 3,026,092 - HSERO_RS13280 0.17 -0.9 3,026,461 - HSERO_RS13280 0.64 +1.1 3,026,524 + HSERO_RS13280 0.72 -0.4 3,027,007 + HSERO_RS13285 0.36 -1.5 3,027,410 - HSERO_RS13285 0.89 -2.3 3,027,565 - -0.7 3,027,618 + +0.4
Or see this region's nucleotide sequence