Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12160 and HSERO_RS12165 are separated by 23 nucleotides HSERO_RS12165 and HSERO_RS12170 are separated by 390 nucleotides HSERO_RS12170 and HSERO_RS12175 overlap by 4 nucleotides
HSERO_RS12160: HSERO_RS12160 - Fe2+-dicitrate sensor membrane protein, at 2,761,439 to 2,762,467
_RS12160
HSERO_RS12165: HSERO_RS12165 - RNA polymerase sigma factor, at 2,762,491 to 2,763,015
_RS12165
HSERO_RS12170: HSERO_RS12170 - XRE family transcriptional regulator, at 2,763,406 to 2,764,068
_RS12170
HSERO_RS12175: HSERO_RS12175 - histidine kinase, at 2,764,065 to 2,765,384
_RS12175
Position (kb)
2763
2764
2765 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2762.645 kb on + strand, within HSERO_RS12165 at 2762.816 kb on + strand, within HSERO_RS12165 at 2762.898 kb on + strand, within HSERO_RS12165 at 2763.092 kb on + strand at 2763.101 kb on + strand at 2763.109 kb on - strand at 2763.134 kb on - strand at 2763.151 kb on - strand at 2763.207 kb on - strand at 2763.226 kb on - strand at 2763.249 kb on - strand at 2763.249 kb on - strand at 2763.259 kb on - strand at 2763.342 kb on + strand at 2763.378 kb on + strand at 2763.516 kb on + strand, within HSERO_RS12170 at 2763.638 kb on - strand, within HSERO_RS12170 at 2763.857 kb on - strand, within HSERO_RS12170 at 2764.037 kb on - strand at 2764.097 kb on + strand at 2764.134 kb on - strand at 2764.296 kb on + strand, within HSERO_RS12175 at 2764.375 kb on + strand, within HSERO_RS12175 at 2764.512 kb on - strand, within HSERO_RS12175 at 2764.512 kb on - strand, within HSERO_RS12175 at 2764.662 kb on + strand, within HSERO_RS12175 at 2764.767 kb on + strand, within HSERO_RS12175 at 2764.775 kb on - strand, within HSERO_RS12175
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 2,762,645 + HSERO_RS12165 0.29 -0.7 2,762,816 + HSERO_RS12165 0.62 -0.0 2,762,898 + HSERO_RS12165 0.78 +0.3 2,763,092 + +0.2 2,763,101 + +0.8 2,763,109 - -1.9 2,763,134 - -0.6 2,763,151 - +0.3 2,763,207 - -0.5 2,763,226 - +0.1 2,763,249 - -0.2 2,763,249 - +1.8 2,763,259 - -0.2 2,763,342 + +0.5 2,763,378 + -0.3 2,763,516 + HSERO_RS12170 0.17 -0.4 2,763,638 - HSERO_RS12170 0.35 -1.4 2,763,857 - HSERO_RS12170 0.68 -0.3 2,764,037 - -1.4 2,764,097 + -0.0 2,764,134 - +0.1 2,764,296 + HSERO_RS12175 0.17 +0.9 2,764,375 + HSERO_RS12175 0.23 +0.1 2,764,512 - HSERO_RS12175 0.34 +0.7 2,764,512 - HSERO_RS12175 0.34 -0.0 2,764,662 + HSERO_RS12175 0.45 -0.1 2,764,767 + HSERO_RS12175 0.53 +0.9 2,764,775 - HSERO_RS12175 0.54 -0.3
Or see this region's nucleotide sequence