Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10005 and HSERO_RS10010 are separated by 96 nucleotides HSERO_RS10010 and HSERO_RS10015 are separated by 124 nucleotides HSERO_RS10015 and HSERO_RS10020 overlap by 1 nucleotides
HSERO_RS10005: HSERO_RS10005 - glycosyl transferase family protein, at 2,264,441 to 2,265,343
_RS10005
HSERO_RS10010: HSERO_RS10010 - UDP-glucose 6-dehydrogenase, at 2,265,440 to 2,266,816
_RS10010
HSERO_RS10015: HSERO_RS10015 - hypothetical protein, at 2,266,941 to 2,267,687
_RS10015
HSERO_RS10020: HSERO_RS10020 - flippase, at 2,267,687 to 2,269,117
_RS10020
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-1
0
1
2 at 2264.506 kb on + strand at 2264.615 kb on + strand, within HSERO_RS10005 at 2265.125 kb on - strand, within HSERO_RS10005 at 2265.129 kb on + strand, within HSERO_RS10005 at 2265.133 kb on + strand, within HSERO_RS10005 at 2265.147 kb on - strand, within HSERO_RS10005 at 2265.157 kb on - strand, within HSERO_RS10005 at 2265.240 kb on + strand, within HSERO_RS10005 at 2265.248 kb on - strand, within HSERO_RS10005 at 2265.395 kb on - strand at 2265.423 kb on - strand at 2265.454 kb on + strand at 2265.478 kb on + strand at 2265.527 kb on + strand at 2266.338 kb on + strand at 2266.421 kb on + strand, within HSERO_RS10010 at 2266.443 kb on - strand, within HSERO_RS10010 at 2266.468 kb on - strand, within HSERO_RS10010 at 2267.185 kb on + strand, within HSERO_RS10015 at 2267.689 kb on + strand at 2267.697 kb on - strand at 2267.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 2,264,506 + +0.1 2,264,615 + HSERO_RS10005 0.19 +0.7 2,265,125 - HSERO_RS10005 0.76 -1.0 2,265,129 + HSERO_RS10005 0.76 -0.3 2,265,133 + HSERO_RS10005 0.77 +0.1 2,265,147 - HSERO_RS10005 0.78 -0.3 2,265,157 - HSERO_RS10005 0.79 +0.0 2,265,240 + HSERO_RS10005 0.88 -0.1 2,265,248 - HSERO_RS10005 0.89 -0.8 2,265,395 - -0.3 2,265,423 - +0.4 2,265,454 + -1.5 2,265,478 + -0.2 2,265,527 + -0.5 2,266,338 + +1.8 2,266,421 + HSERO_RS10010 0.71 +0.7 2,266,443 - HSERO_RS10010 0.73 +0.6 2,266,468 - HSERO_RS10010 0.75 -0.1 2,267,185 + HSERO_RS10015 0.33 +0.3 2,267,689 + +0.6 2,267,697 - +0.7 2,267,789 - +0.5
Or see this region's nucleotide sequence