Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS09905 and HSERO_RS09910 are separated by 124 nucleotides HSERO_RS09910 and HSERO_RS09915 are separated by 3 nucleotides HSERO_RS09915 and HSERO_RS09920 are separated by 158 nucleotides HSERO_RS09920 and HSERO_RS09925 are separated by 72 nucleotides
HSERO_RS09905: HSERO_RS09905 - phenylalanyl-tRNA synthetase subunit beta, at 2,238,959 to 2,241,385
_RS09905
HSERO_RS09910: HSERO_RS09910 - integration host factor subunit alpha, at 2,241,510 to 2,241,863
_RS09910
HSERO_RS09915: HSERO_RS09915 - transcriptional regulator, at 2,241,867 to 2,242,274
_RS09915
HSERO_RS09920: HSERO_RS09920 - acetyltransferase, at 2,242,433 to 2,242,942
_RS09920
HSERO_RS09925: HSERO_RS09925 - hypothetical protein, at 2,243,015 to 2,243,641
_RS09925
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-1
0
1
2 at 2241.817 kb on + strand, within HSERO_RS09910 at 2241.934 kb on + strand, within HSERO_RS09915 at 2242.268 kb on + strand at 2242.615 kb on - strand, within HSERO_RS09920 at 2242.817 kb on + strand, within HSERO_RS09920 at 2243.059 kb on + strand at 2243.107 kb on + strand, within HSERO_RS09925 at 2243.193 kb on - strand, within HSERO_RS09925
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 2,241,817 + HSERO_RS09910 0.87 -0.6 2,241,934 + HSERO_RS09915 0.16 -0.8 2,242,268 + -0.1 2,242,615 - HSERO_RS09920 0.36 -0.1 2,242,817 + HSERO_RS09920 0.75 -0.2 2,243,059 + -1.4 2,243,107 + HSERO_RS09925 0.15 +2.7 2,243,193 - HSERO_RS09925 0.28 -0.4
Or see this region's nucleotide sequence