Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS09845 and HSERO_RS09850 are separated by 96 nucleotides HSERO_RS09850 and HSERO_RS09855 are separated by 6 nucleotides HSERO_RS09855 and HSERO_RS09860 are separated by 105 nucleotides HSERO_RS09860 and HSERO_RS09865 are separated by 91 nucleotides HSERO_RS09865 and HSERO_RS09870 are separated by 328 nucleotides
HSERO_RS09845: HSERO_RS09845 - alpha/beta hydrolase, at 2,228,847 to 2,229,713
_RS09845
HSERO_RS09850: HSERO_RS09850 - aminoacrylate peracid reductase, at 2,229,810 to 2,230,160
_RS09850
HSERO_RS09855: HSERO_RS09855 - GTP pyrophosphokinase, at 2,230,167 to 2,232,416
_RS09855
HSERO_RS09860: HSERO_RS09860 - tRNA-Val, at 2,232,522 to 2,232,598
_RS09860
HSERO_RS09865: HSERO_RS09865 - tRNA-Val, at 2,232,690 to 2,232,766
_RS09865
HSERO_RS09870: HSERO_RS09870 - chemotaxis protein CheY, at 2,233,095 to 2,233,502
_RS09870
Position (kb)
2230
2231
2232
2233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2229.345 kb on + strand at 2229.382 kb on + strand, within HSERO_RS09845 at 2229.481 kb on - strand, within HSERO_RS09845 at 2229.481 kb on - strand, within HSERO_RS09845 at 2229.538 kb on + strand, within HSERO_RS09845 at 2229.776 kb on + strand at 2229.784 kb on - strand at 2229.929 kb on - strand, within HSERO_RS09850 at 2229.936 kb on + strand, within HSERO_RS09850 at 2230.149 kb on + strand at 2230.933 kb on - strand, within HSERO_RS09855 at 2231.055 kb on + strand, within HSERO_RS09855 at 2231.055 kb on + strand, within HSERO_RS09855 at 2231.407 kb on + strand, within HSERO_RS09855 at 2232.383 kb on + strand at 2232.915 kb on + strand at 2232.934 kb on - strand at 2232.992 kb on - strand at 2232.992 kb on - strand at 2233.302 kb on - strand, within HSERO_RS09870 at 2233.304 kb on + strand, within HSERO_RS09870 at 2233.352 kb on + strand, within HSERO_RS09870 at 2233.395 kb on + strand, within HSERO_RS09870 at 2233.395 kb on + strand, within HSERO_RS09870
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 2,229,345 + -1.7 2,229,382 + HSERO_RS09845 0.62 -0.8 2,229,481 - HSERO_RS09845 0.73 +0.2 2,229,481 - HSERO_RS09845 0.73 -0.3 2,229,538 + HSERO_RS09845 0.80 +1.6 2,229,776 + +0.0 2,229,784 - -0.1 2,229,929 - HSERO_RS09850 0.34 +2.1 2,229,936 + HSERO_RS09850 0.36 -1.9 2,230,149 + -0.5 2,230,933 - HSERO_RS09855 0.34 +2.1 2,231,055 + HSERO_RS09855 0.39 -0.5 2,231,055 + HSERO_RS09855 0.39 -1.4 2,231,407 + HSERO_RS09855 0.55 +2.4 2,232,383 + +0.5 2,232,915 + +2.5 2,232,934 - -0.4 2,232,992 - -1.3 2,232,992 - -2.4 2,233,302 - HSERO_RS09870 0.51 +0.3 2,233,304 + HSERO_RS09870 0.51 +0.3 2,233,352 + HSERO_RS09870 0.63 +0.8 2,233,395 + HSERO_RS09870 0.74 +0.6 2,233,395 + HSERO_RS09870 0.74 +0.7
Or see this region's nucleotide sequence