Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS09720

Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS09695 and HSERO_RS09700 are separated by 70 nucleotidesHSERO_RS09700 and HSERO_RS09705 are separated by 152 nucleotidesHSERO_RS09705 and HSERO_RS09710 are separated by 11 nucleotidesHSERO_RS09710 and HSERO_RS09715 are separated by 54 nucleotidesHSERO_RS09715 and HSERO_RS09720 are separated by 121 nucleotidesHSERO_RS09720 and HSERO_RS09725 are separated by 1 nucleotides HSERO_RS09695: HSERO_RS09695 - CDP-diacylglycerol/glycerol-3-phosphate 3-phosphatidyltransferase, at 2,200,315 to 2,200,914 _RS09695 HSERO_RS09700: HSERO_RS09700 - tRNA-Gly, at 2,200,985 to 2,201,060 _RS09700 HSERO_RS09705: HSERO_RS09705 - tRNA-Cys, at 2,201,213 to 2,201,286 _RS09705 HSERO_RS09710: HSERO_RS09710 - tRNA-Gly, at 2,201,298 to 2,201,373 _RS09710 HSERO_RS09715: HSERO_RS09715 - tRNA-Gly, at 2,201,428 to 2,201,503 _RS09715 HSERO_RS09720: HSERO_RS09720 - membrane protein, at 2,201,625 to 2,202,752 _RS09720 HSERO_RS09725: HSERO_RS09725 - ABC transporter, at 2,202,754 to 2,205,495 _RS09725 Position (kb) 2201 2202 2203Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2201.756 kb on + strand, within HSERO_RS09720at 2202.153 kb on + strand, within HSERO_RS09720at 2202.227 kb on - strand, within HSERO_RS09720at 2202.741 kb on + strandat 2202.949 kb on - strandat 2203.211 kb on + strand, within HSERO_RS09725at 2203.620 kb on + strandat 2203.729 kb on - strand, within HSERO_RS09725

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
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2,201,756 + HSERO_RS09720 0.12 -1.9
2,202,153 + HSERO_RS09720 0.47 -0.7
2,202,227 - HSERO_RS09720 0.53 -2.5
2,202,741 + -0.7
2,202,949 - +1.1
2,203,211 + HSERO_RS09725 0.17 -3.1
2,203,620 + +1.3
2,203,729 - HSERO_RS09725 0.36 -4.2

Or see this region's nucleotide sequence