Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS09695 and HSERO_RS09700 are separated by 70 nucleotides HSERO_RS09700 and HSERO_RS09705 are separated by 152 nucleotides HSERO_RS09705 and HSERO_RS09710 are separated by 11 nucleotides HSERO_RS09710 and HSERO_RS09715 are separated by 54 nucleotides HSERO_RS09715 and HSERO_RS09720 are separated by 121 nucleotides HSERO_RS09720 and HSERO_RS09725 are separated by 1 nucleotides
HSERO_RS09695: HSERO_RS09695 - CDP-diacylglycerol/glycerol-3-phosphate 3-phosphatidyltransferase, at 2,200,315 to 2,200,914
_RS09695
HSERO_RS09700: HSERO_RS09700 - tRNA-Gly, at 2,200,985 to 2,201,060
_RS09700
HSERO_RS09705: HSERO_RS09705 - tRNA-Cys, at 2,201,213 to 2,201,286
_RS09705
HSERO_RS09710: HSERO_RS09710 - tRNA-Gly, at 2,201,298 to 2,201,373
_RS09710
HSERO_RS09715: HSERO_RS09715 - tRNA-Gly, at 2,201,428 to 2,201,503
_RS09715
HSERO_RS09720: HSERO_RS09720 - membrane protein, at 2,201,625 to 2,202,752
_RS09720
HSERO_RS09725: HSERO_RS09725 - ABC transporter, at 2,202,754 to 2,205,495
_RS09725
Position (kb)
2201
2202
2203 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2201.756 kb on + strand, within HSERO_RS09720 at 2202.153 kb on + strand, within HSERO_RS09720 at 2202.227 kb on - strand, within HSERO_RS09720 at 2202.741 kb on + strand at 2202.949 kb on - strand at 2203.211 kb on + strand, within HSERO_RS09725 at 2203.620 kb on + strand at 2203.729 kb on - strand, within HSERO_RS09725
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 2,201,756 + HSERO_RS09720 0.12 -1.9 2,202,153 + HSERO_RS09720 0.47 -0.7 2,202,227 - HSERO_RS09720 0.53 -2.5 2,202,741 + -0.7 2,202,949 - +1.1 2,203,211 + HSERO_RS09725 0.17 -3.1 2,203,620 + +1.3 2,203,729 - HSERO_RS09725 0.36 -4.2
Or see this region's nucleotide sequence