Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS07300

Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS07295 and HSERO_RS07300 are separated by 265 nucleotidesHSERO_RS07300 and HSERO_RS07305 are separated by 556 nucleotides HSERO_RS07295: HSERO_RS07295 - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase, at 1,669,392 to 1,670,237 _RS07295 HSERO_RS07300: HSERO_RS07300 - glutamate--cysteine ligase, at 1,670,503 to 1,672,098 _RS07300 HSERO_RS07305: HSERO_RS07305 - pyruvate dehydrogenase, at 1,672,655 to 1,675,351 _RS07305 Position (kb) 1670 1671 1672 1673Strain fitness (log2 ratio) -1 0 1at 1670.471 kb on + strandat 1670.493 kb on + strandat 1670.624 kb on - strandat 1670.721 kb on + strand, within HSERO_RS07300at 1671.292 kb on + strand, within HSERO_RS07300at 1671.391 kb on - strand, within HSERO_RS07300at 1672.041 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
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1,670,471 + +0.1
1,670,493 + -0.0
1,670,624 - -0.8
1,670,721 + HSERO_RS07300 0.14 -0.6
1,671,292 + HSERO_RS07300 0.49 -0.1
1,671,391 - HSERO_RS07300 0.56 -1.6
1,672,041 + -0.3

Or see this region's nucleotide sequence