Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02315 and HSERO_RS02320 are separated by 14 nucleotides HSERO_RS02320 and HSERO_RS02325 overlap by 4 nucleotides HSERO_RS02325 and HSERO_RS02330 overlap by 8 nucleotides HSERO_RS02330 and HSERO_RS02335 are separated by 175 nucleotides HSERO_RS02335 and HSERO_RS02340 overlap by 1 nucleotides
HSERO_RS02315: HSERO_RS02315 - transcriptional regulator, at 507,140 to 507,844
_RS02315
HSERO_RS02320: HSERO_RS02320 - Holliday junction resolvase, at 507,859 to 508,245
_RS02320
HSERO_RS02325: HSERO_RS02325 - uracil phosphoribosyltransferase, at 508,242 to 508,754
_RS02325
HSERO_RS02330: HSERO_RS02330 - aspartate carbamoyltransferase, at 508,747 to 509,706
_RS02330
HSERO_RS02335: HSERO_RS02335 - excinuclease ABC subunit C, at 509,882 to 510,166
_RS02335
HSERO_RS02340: HSERO_RS02340 - dihydroorotase, at 510,166 to 511,452
_RS02340
Position (kb) 508
509
510 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 508.254 kb on - strand at 508.288 kb on - strand at 508.402 kb on - strand, within HSERO_RS02325 at 508.535 kb on + strand, within HSERO_RS02325 at 508.543 kb on - strand, within HSERO_RS02325 at 508.543 kb on - strand, within HSERO_RS02325 at 508.641 kb on - strand, within HSERO_RS02325 at 508.749 kb on + strand at 508.817 kb on - strand at 508.843 kb on + strand, within HSERO_RS02330 at 509.011 kb on - strand, within HSERO_RS02330 at 509.035 kb on + strand, within HSERO_RS02330 at 509.043 kb on - strand, within HSERO_RS02330 at 509.222 kb on - strand at 509.341 kb on - strand, within HSERO_RS02330 at 509.419 kb on - strand, within HSERO_RS02330 at 509.547 kb on + strand, within HSERO_RS02330 at 509.582 kb on - strand, within HSERO_RS02330 at 509.688 kb on - strand at 509.787 kb on - strand at 509.798 kb on + strand at 509.806 kb on - strand at 509.910 kb on + strand at 509.923 kb on + strand, within HSERO_RS02335 at 509.923 kb on + strand, within HSERO_RS02335 at 509.942 kb on - strand, within HSERO_RS02335 at 509.997 kb on - strand, within HSERO_RS02335 at 510.308 kb on + strand, within HSERO_RS02340 at 510.375 kb on + strand, within HSERO_RS02340 at 510.412 kb on + strand, within HSERO_RS02340 at 510.525 kb on + strand, within HSERO_RS02340 at 510.533 kb on - strand, within HSERO_RS02340 at 510.567 kb on + strand, within HSERO_RS02340 at 510.576 kb on - strand, within HSERO_RS02340 at 510.576 kb on - strand, within HSERO_RS02340 at 510.645 kb on - strand, within HSERO_RS02340
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 508,254 - -4.2 508,288 - -4.3 508,402 - HSERO_RS02325 0.31 -3.1 508,535 + HSERO_RS02325 0.57 +2.9 508,543 - HSERO_RS02325 0.59 -3.1 508,543 - HSERO_RS02325 0.59 -2.2 508,641 - HSERO_RS02325 0.78 -2.6 508,749 + -5.5 508,817 - -3.6 508,843 + HSERO_RS02330 0.10 -2.5 509,011 - HSERO_RS02330 0.28 -5.0 509,035 + HSERO_RS02330 0.30 -4.5 509,043 - HSERO_RS02330 0.31 -5.0 509,222 - -0.9 509,341 - HSERO_RS02330 0.62 -6.2 509,419 - HSERO_RS02330 0.70 -2.5 509,547 + HSERO_RS02330 0.83 -3.7 509,582 - HSERO_RS02330 0.87 -3.2 509,688 - -6.9 509,787 - -0.8 509,798 + +0.8 509,806 - -2.8 509,910 + +0.3 509,923 + HSERO_RS02335 0.14 -0.4 509,923 + HSERO_RS02335 0.14 -0.6 509,942 - HSERO_RS02335 0.21 -0.1 509,997 - HSERO_RS02335 0.40 -1.6 510,308 + HSERO_RS02340 0.11 -2.8 510,375 + HSERO_RS02340 0.16 -4.4 510,412 + HSERO_RS02340 0.19 -3.4 510,525 + HSERO_RS02340 0.28 -4.9 510,533 - HSERO_RS02340 0.29 -4.8 510,567 + HSERO_RS02340 0.31 -3.8 510,576 - HSERO_RS02340 0.32 -3.4 510,576 - HSERO_RS02340 0.32 -3.7 510,645 - HSERO_RS02340 0.37 -3.8
Or see this region's nucleotide sequence