Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00170 and HSERO_RS00175 are separated by 16 nucleotides HSERO_RS00175 and HSERO_RS00180 are separated by 134 nucleotides HSERO_RS00180 and HSERO_RS00185 are separated by 253 nucleotides
HSERO_RS00170: HSERO_RS00170 - Fe2+-dicitrate sensor membrane protein, at 46,587 to 47,597
_RS00170
HSERO_RS00175: HSERO_RS00175 - RNA polymerase sigma factor, at 47,614 to 48,120
_RS00175
HSERO_RS00180: HSERO_RS00180 - hypothetical protein, at 48,255 to 48,743
_RS00180
HSERO_RS00185: HSERO_RS00185 - MFS transporter, at 48,997 to 50,235
_RS00185
Position (kb)
48
49 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 47.265 kb on + strand, within HSERO_RS00170 at 47.303 kb on + strand, within HSERO_RS00170 at 47.439 kb on + strand, within HSERO_RS00170 at 47.447 kb on - strand, within HSERO_RS00170 at 47.454 kb on + strand, within HSERO_RS00170 at 47.469 kb on + strand, within HSERO_RS00170 at 47.665 kb on - strand, within HSERO_RS00175 at 47.984 kb on - strand, within HSERO_RS00175 at 48.086 kb on + strand at 48.128 kb on - strand at 48.146 kb on + strand at 48.168 kb on + strand at 48.168 kb on + strand at 48.212 kb on + strand at 48.279 kb on - strand at 48.289 kb on - strand at 48.306 kb on + strand, within HSERO_RS00180 at 48.314 kb on - strand, within HSERO_RS00180 at 48.314 kb on - strand, within HSERO_RS00180 at 48.420 kb on - strand, within HSERO_RS00180 at 48.486 kb on - strand at 48.768 kb on - strand at 48.956 kb on - strand at 48.963 kb on - strand at 48.988 kb on + strand at 49.108 kb on + strand at 49.108 kb on + strand at 49.116 kb on - strand at 49.116 kb on - strand at 49.201 kb on - strand, within HSERO_RS00185 at 49.239 kb on + strand, within HSERO_RS00185 at 49.286 kb on - strand, within HSERO_RS00185 at 49.330 kb on + strand, within HSERO_RS00185 at 49.359 kb on + strand, within HSERO_RS00185 at 49.451 kb on + strand, within HSERO_RS00185
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 47,265 + HSERO_RS00170 0.67 +0.2 47,303 + HSERO_RS00170 0.71 -0.2 47,439 + HSERO_RS00170 0.84 +1.0 47,447 - HSERO_RS00170 0.85 +0.3 47,454 + HSERO_RS00170 0.86 +0.1 47,469 + HSERO_RS00170 0.87 -0.3 47,665 - HSERO_RS00175 0.10 +1.6 47,984 - HSERO_RS00175 0.73 +0.2 48,086 + -1.3 48,128 - -0.4 48,146 + +0.2 48,168 + -1.0 48,168 + -0.2 48,212 + -0.8 48,279 - -1.0 48,289 - -1.3 48,306 + HSERO_RS00180 0.10 -0.8 48,314 - HSERO_RS00180 0.12 -0.8 48,314 - HSERO_RS00180 0.12 -0.5 48,420 - HSERO_RS00180 0.34 -0.6 48,486 - +1.9 48,768 - -0.1 48,956 - -2.0 48,963 - +0.9 48,988 + -1.5 49,108 + -0.7 49,108 + +0.4 49,116 - -0.1 49,116 - +0.1 49,201 - HSERO_RS00185 0.16 +0.8 49,239 + HSERO_RS00185 0.20 -1.2 49,286 - HSERO_RS00185 0.23 -0.5 49,330 + HSERO_RS00185 0.27 -0.4 49,359 + HSERO_RS00185 0.29 -1.8 49,451 + HSERO_RS00185 0.37 -0.4
Or see this region's nucleotide sequence