Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25400 and AO356_25405 are separated by 181 nucleotides AO356_25405 and AO356_25410 are separated by 96 nucleotides
AO356_25400: AO356_25400 - acetylornithine deacetylase, at 5,744,535 to 5,745,704
_25400
AO356_25405: AO356_25405 - spermidine/putrescine ABC transporter substrate-binding protein, at 5,745,886 to 5,746,926
_25405
AO356_25410: AO356_25410 - LysR family transcriptional regulator, at 5,747,023 to 5,747,934
_25410
Position (kb)
5745
5746
5747 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5744.993 kb on + strand, within AO356_25400 at 5745.112 kb on - strand, within AO356_25400 at 5745.310 kb on + strand, within AO356_25400 at 5745.364 kb on - strand, within AO356_25400 at 5745.364 kb on - strand, within AO356_25400 at 5745.621 kb on + strand at 5745.622 kb on - strand at 5745.678 kb on + strand at 5745.679 kb on - strand at 5745.800 kb on + strand at 5745.800 kb on + strand at 5745.817 kb on - strand at 5745.820 kb on + strand at 5745.821 kb on - strand at 5745.821 kb on - strand at 5745.909 kb on + strand at 5746.016 kb on - strand, within AO356_25405 at 5746.066 kb on + strand, within AO356_25405 at 5746.219 kb on + strand, within AO356_25405 at 5746.219 kb on + strand, within AO356_25405 at 5746.219 kb on + strand, within AO356_25405 at 5746.219 kb on + strand, within AO356_25405 at 5746.219 kb on + strand, within AO356_25405 at 5746.220 kb on - strand, within AO356_25405 at 5746.258 kb on + strand, within AO356_25405 at 5746.259 kb on - strand, within AO356_25405 at 5746.259 kb on - strand, within AO356_25405 at 5746.382 kb on - strand, within AO356_25405 at 5746.432 kb on + strand, within AO356_25405 at 5746.433 kb on - strand, within AO356_25405 at 5746.433 kb on - strand, within AO356_25405 at 5746.760 kb on - strand, within AO356_25405 at 5746.925 kb on - strand at 5746.979 kb on + strand at 5746.980 kb on - strand at 5746.980 kb on - strand at 5746.983 kb on + strand at 5746.983 kb on + strand at 5747.174 kb on + strand, within AO356_25410 at 5747.174 kb on + strand, within AO356_25410 at 5747.175 kb on - strand, within AO356_25410 at 5747.463 kb on + strand, within AO356_25410 at 5747.464 kb on - strand, within AO356_25410 at 5747.849 kb on + strand at 5747.924 kb on + strand at 5747.925 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,744,993 + AO356_25400 0.39 +2.2 5,745,112 - AO356_25400 0.49 -0.2 5,745,310 + AO356_25400 0.66 -1.8 5,745,364 - AO356_25400 0.71 -0.7 5,745,364 - AO356_25400 0.71 -0.4 5,745,621 + -0.3 5,745,622 - +0.1 5,745,678 + +1.7 5,745,679 - -0.1 5,745,800 + -1.2 5,745,800 + -0.5 5,745,817 - +1.1 5,745,820 + -0.0 5,745,821 - +1.2 5,745,821 - -0.1 5,745,909 + +0.1 5,746,016 - AO356_25405 0.12 +0.5 5,746,066 + AO356_25405 0.17 +1.0 5,746,219 + AO356_25405 0.32 +0.6 5,746,219 + AO356_25405 0.32 +0.6 5,746,219 + AO356_25405 0.32 +0.7 5,746,219 + AO356_25405 0.32 +0.3 5,746,219 + AO356_25405 0.32 -0.1 5,746,220 - AO356_25405 0.32 +1.3 5,746,258 + AO356_25405 0.36 +0.3 5,746,259 - AO356_25405 0.36 +0.3 5,746,259 - AO356_25405 0.36 +0.1 5,746,382 - AO356_25405 0.48 -1.1 5,746,432 + AO356_25405 0.52 +0.3 5,746,433 - AO356_25405 0.53 -1.7 5,746,433 - AO356_25405 0.53 +1.7 5,746,760 - AO356_25405 0.84 +0.3 5,746,925 - +0.8 5,746,979 + -1.4 5,746,980 - -0.7 5,746,980 - +0.2 5,746,983 + -0.5 5,746,983 + +0.6 5,747,174 + AO356_25410 0.17 +1.0 5,747,174 + AO356_25410 0.17 -0.1 5,747,175 - AO356_25410 0.17 -0.0 5,747,463 + AO356_25410 0.48 -0.3 5,747,464 - AO356_25410 0.48 +0.2 5,747,849 + +0.2 5,747,924 + +0.2 5,747,925 - +1.0
Or see this region's nucleotide sequence