Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_25405

Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_25400 and AO356_25405 are separated by 181 nucleotidesAO356_25405 and AO356_25410 are separated by 96 nucleotides AO356_25400: AO356_25400 - acetylornithine deacetylase, at 5,744,535 to 5,745,704 _25400 AO356_25405: AO356_25405 - spermidine/putrescine ABC transporter substrate-binding protein, at 5,745,886 to 5,746,926 _25405 AO356_25410: AO356_25410 - LysR family transcriptional regulator, at 5,747,023 to 5,747,934 _25410 Position (kb) 5745 5746 5747Strain fitness (log2 ratio) -2 -1 0 1 2at 5744.993 kb on + strand, within AO356_25400at 5745.112 kb on - strand, within AO356_25400at 5745.310 kb on + strand, within AO356_25400at 5745.364 kb on - strand, within AO356_25400at 5745.364 kb on - strand, within AO356_25400at 5745.621 kb on + strandat 5745.622 kb on - strandat 5745.678 kb on + strandat 5745.679 kb on - strandat 5745.800 kb on + strandat 5745.800 kb on + strandat 5745.817 kb on - strandat 5745.820 kb on + strandat 5745.821 kb on - strandat 5745.821 kb on - strandat 5745.909 kb on + strandat 5746.016 kb on - strand, within AO356_25405at 5746.066 kb on + strand, within AO356_25405at 5746.219 kb on + strand, within AO356_25405at 5746.219 kb on + strand, within AO356_25405at 5746.219 kb on + strand, within AO356_25405at 5746.219 kb on + strand, within AO356_25405at 5746.219 kb on + strand, within AO356_25405at 5746.220 kb on - strand, within AO356_25405at 5746.258 kb on + strand, within AO356_25405at 5746.259 kb on - strand, within AO356_25405at 5746.259 kb on - strand, within AO356_25405at 5746.382 kb on - strand, within AO356_25405at 5746.432 kb on + strand, within AO356_25405at 5746.433 kb on - strand, within AO356_25405at 5746.433 kb on - strand, within AO356_25405at 5746.760 kb on - strand, within AO356_25405at 5746.925 kb on - strandat 5746.979 kb on + strandat 5746.980 kb on - strandat 5746.980 kb on - strandat 5746.983 kb on + strandat 5746.983 kb on + strandat 5747.174 kb on + strand, within AO356_25410at 5747.174 kb on + strand, within AO356_25410at 5747.175 kb on - strand, within AO356_25410at 5747.463 kb on + strand, within AO356_25410at 5747.464 kb on - strand, within AO356_25410at 5747.849 kb on + strandat 5747.924 kb on + strandat 5747.925 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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5,744,993 + AO356_25400 0.39 +2.2
5,745,112 - AO356_25400 0.49 -0.2
5,745,310 + AO356_25400 0.66 -1.8
5,745,364 - AO356_25400 0.71 -0.7
5,745,364 - AO356_25400 0.71 -0.4
5,745,621 + -0.3
5,745,622 - +0.1
5,745,678 + +1.7
5,745,679 - -0.1
5,745,800 + -1.2
5,745,800 + -0.5
5,745,817 - +1.1
5,745,820 + -0.0
5,745,821 - +1.2
5,745,821 - -0.1
5,745,909 + +0.1
5,746,016 - AO356_25405 0.12 +0.5
5,746,066 + AO356_25405 0.17 +1.0
5,746,219 + AO356_25405 0.32 +0.6
5,746,219 + AO356_25405 0.32 +0.6
5,746,219 + AO356_25405 0.32 +0.7
5,746,219 + AO356_25405 0.32 +0.3
5,746,219 + AO356_25405 0.32 -0.1
5,746,220 - AO356_25405 0.32 +1.3
5,746,258 + AO356_25405 0.36 +0.3
5,746,259 - AO356_25405 0.36 +0.3
5,746,259 - AO356_25405 0.36 +0.1
5,746,382 - AO356_25405 0.48 -1.1
5,746,432 + AO356_25405 0.52 +0.3
5,746,433 - AO356_25405 0.53 -1.7
5,746,433 - AO356_25405 0.53 +1.7
5,746,760 - AO356_25405 0.84 +0.3
5,746,925 - +0.8
5,746,979 + -1.4
5,746,980 - -0.7
5,746,980 - +0.2
5,746,983 + -0.5
5,746,983 + +0.6
5,747,174 + AO356_25410 0.17 +1.0
5,747,174 + AO356_25410 0.17 -0.1
5,747,175 - AO356_25410 0.17 -0.0
5,747,463 + AO356_25410 0.48 -0.3
5,747,464 - AO356_25410 0.48 +0.2
5,747,849 + +0.2
5,747,924 + +0.2
5,747,925 - +1.0

Or see this region's nucleotide sequence