Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23550 and AO356_23555 are separated by 7 nucleotides AO356_23555 and AO356_23560 are separated by 52 nucleotides AO356_23560 and AO356_23565 are separated by 102 nucleotides
AO356_23550: AO356_23550 - aliphatic sulfonate ABC transporter substrate-binding protein, at 5,279,338 to 5,280,339
_23550
AO356_23555: AO356_23555 - ABC transporter permease, at 5,280,347 to 5,281,096
_23555
AO356_23560: AO356_23560 - hypothetical protein, at 5,281,149 to 5,283,518
_23560
AO356_23565: AO356_23565 - peptidase, at 5,283,621 to 5,285,405
_23565
Position (kb) 5281
5282
5283
5284 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5280.310 kb on - strand at 5280.316 kb on + strand at 5280.317 kb on - strand at 5280.434 kb on + strand, within AO356_23555 at 5280.434 kb on + strand, within AO356_23555 at 5280.434 kb on + strand, within AO356_23555 at 5280.434 kb on + strand, within AO356_23555 at 5280.435 kb on - strand, within AO356_23555 at 5280.435 kb on - strand, within AO356_23555 at 5280.696 kb on - strand, within AO356_23555 at 5280.842 kb on + strand, within AO356_23555 at 5281.073 kb on + strand at 5281.094 kb on + strand at 5281.095 kb on - strand at 5281.095 kb on - strand at 5281.185 kb on + strand at 5281.185 kb on + strand at 5281.185 kb on + strand at 5281.185 kb on + strand at 5281.186 kb on - strand at 5281.186 kb on - strand at 5281.186 kb on - strand at 5281.300 kb on - strand at 5281.359 kb on + strand at 5281.408 kb on - strand, within AO356_23560 at 5281.408 kb on - strand, within AO356_23560 at 5281.408 kb on - strand, within AO356_23560 at 5281.408 kb on - strand, within AO356_23560 at 5281.422 kb on + strand, within AO356_23560 at 5282.109 kb on + strand, within AO356_23560 at 5282.109 kb on + strand, within AO356_23560 at 5282.110 kb on - strand, within AO356_23560 at 5282.110 kb on - strand, within AO356_23560 at 5282.110 kb on - strand, within AO356_23560 at 5282.110 kb on - strand, within AO356_23560 at 5282.110 kb on - strand, within AO356_23560 at 5282.110 kb on - strand, within AO356_23560 at 5282.122 kb on - strand, within AO356_23560 at 5282.178 kb on - strand, within AO356_23560 at 5282.430 kb on + strand, within AO356_23560 at 5282.430 kb on + strand, within AO356_23560 at 5282.431 kb on - strand, within AO356_23560 at 5282.431 kb on - strand, within AO356_23560 at 5282.440 kb on - strand, within AO356_23560 at 5283.013 kb on - strand, within AO356_23560 at 5283.193 kb on + strand, within AO356_23560 at 5283.194 kb on - strand, within AO356_23560 at 5283.219 kb on + strand, within AO356_23560 at 5283.219 kb on + strand, within AO356_23560 at 5283.219 kb on + strand, within AO356_23560 at 5283.219 kb on + strand, within AO356_23560 at 5283.220 kb on - strand, within AO356_23560 at 5283.220 kb on - strand, within AO356_23560 at 5283.255 kb on + strand, within AO356_23560 at 5283.255 kb on + strand, within AO356_23560 at 5283.255 kb on + strand, within AO356_23560 at 5283.255 kb on + strand, within AO356_23560 at 5283.255 kb on + strand, within AO356_23560 at 5283.583 kb on + strand at 5283.583 kb on + strand at 5283.583 kb on + strand at 5283.583 kb on + strand at 5283.584 kb on - strand at 5283.584 kb on - strand at 5283.584 kb on - strand at 5283.584 kb on - strand at 5283.738 kb on + strand at 5283.738 kb on + strand at 5283.738 kb on + strand at 5283.738 kb on + strand at 5283.738 kb on + strand at 5283.738 kb on + strand at 5283.738 kb on + strand at 5283.739 kb on - strand at 5283.739 kb on - strand at 5283.739 kb on - strand at 5283.739 kb on - strand at 5283.739 kb on - strand at 5283.740 kb on + strand at 5283.741 kb on - strand at 5283.753 kb on + strand at 5283.754 kb on - strand at 5284.054 kb on - strand, within AO356_23565 at 5284.297 kb on + strand, within AO356_23565 at 5284.297 kb on + strand, within AO356_23565 at 5284.297 kb on + strand, within AO356_23565 at 5284.297 kb on + strand, within AO356_23565 at 5284.402 kb on - strand, within AO356_23565
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,280,310 - +2.4 5,280,316 + +0.5 5,280,317 - -0.3 5,280,434 + AO356_23555 0.12 +0.0 5,280,434 + AO356_23555 0.12 +2.2 5,280,434 + AO356_23555 0.12 -0.1 5,280,434 + AO356_23555 0.12 +2.1 5,280,435 - AO356_23555 0.12 -0.1 5,280,435 - AO356_23555 0.12 -0.4 5,280,696 - AO356_23555 0.47 +1.0 5,280,842 + AO356_23555 0.66 -0.2 5,281,073 + +1.0 5,281,094 + -0.2 5,281,095 - -1.1 5,281,095 - +0.5 5,281,185 + -0.1 5,281,185 + +0.1 5,281,185 + -0.8 5,281,185 + -0.4 5,281,186 - +0.0 5,281,186 - +0.7 5,281,186 - -0.2 5,281,300 - -0.5 5,281,359 + +0.4 5,281,408 - AO356_23560 0.11 -0.4 5,281,408 - AO356_23560 0.11 +0.1 5,281,408 - AO356_23560 0.11 -0.9 5,281,408 - AO356_23560 0.11 -0.6 5,281,422 + AO356_23560 0.12 +0.0 5,282,109 + AO356_23560 0.41 +1.2 5,282,109 + AO356_23560 0.41 +0.4 5,282,110 - AO356_23560 0.41 -1.3 5,282,110 - AO356_23560 0.41 +0.8 5,282,110 - AO356_23560 0.41 -0.1 5,282,110 - AO356_23560 0.41 +0.6 5,282,110 - AO356_23560 0.41 -1.1 5,282,110 - AO356_23560 0.41 +0.1 5,282,122 - AO356_23560 0.41 -1.0 5,282,178 - AO356_23560 0.43 +1.8 5,282,430 + AO356_23560 0.54 -0.8 5,282,430 + AO356_23560 0.54 +0.4 5,282,431 - AO356_23560 0.54 -0.2 5,282,431 - AO356_23560 0.54 +0.4 5,282,440 - AO356_23560 0.54 -0.9 5,283,013 - AO356_23560 0.79 -0.2 5,283,193 + AO356_23560 0.86 +0.8 5,283,194 - AO356_23560 0.86 -1.2 5,283,219 + AO356_23560 0.87 +0.2 5,283,219 + AO356_23560 0.87 +0.9 5,283,219 + AO356_23560 0.87 -1.8 5,283,219 + AO356_23560 0.87 -0.9 5,283,220 - AO356_23560 0.87 +0.6 5,283,220 - AO356_23560 0.87 +0.6 5,283,255 + AO356_23560 0.89 -0.8 5,283,255 + AO356_23560 0.89 -0.1 5,283,255 + AO356_23560 0.89 +0.4 5,283,255 + AO356_23560 0.89 -0.7 5,283,255 + AO356_23560 0.89 -0.0 5,283,583 + +0.8 5,283,583 + -0.1 5,283,583 + +1.0 5,283,583 + +0.1 5,283,584 - +0.1 5,283,584 - +0.1 5,283,584 - -0.1 5,283,584 - +0.0 5,283,738 + +0.4 5,283,738 + -1.2 5,283,738 + +0.0 5,283,738 + -0.3 5,283,738 + +0.7 5,283,738 + +0.3 5,283,738 + +0.2 5,283,739 - +0.2 5,283,739 - -0.9 5,283,739 - +0.2 5,283,739 - +0.2 5,283,739 - -1.0 5,283,740 + +0.4 5,283,741 - +0.6 5,283,753 + +0.6 5,283,754 - +0.8 5,284,054 - AO356_23565 0.24 +0.2 5,284,297 + AO356_23565 0.38 -1.0 5,284,297 + AO356_23565 0.38 +0.1 5,284,297 + AO356_23565 0.38 +0.3 5,284,297 + AO356_23565 0.38 -0.7 5,284,402 - AO356_23565 0.44 -0.7
Or see this region's nucleotide sequence