Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23420 and AO356_23425 are separated by 25 nucleotides AO356_23425 and AO356_23430 are separated by 384 nucleotides
AO356_23420: AO356_23420 - carboxynorspermidine decarboxylase, at 5,251,379 to 5,252,476
_23420
AO356_23425: AO356_23425 - saccharopine dehydrogenase, at 5,252,502 to 5,253,746
_23425
AO356_23430: AO356_23430 - NAD-dependent deacetylase, at 5,254,131 to 5,254,886
_23430
Position (kb) 5252
5253
5254 Strain fitness (log2 ratio) -1
0
1
2 at 5251.529 kb on + strand, within AO356_23420 at 5251.529 kb on + strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.607 kb on + strand, within AO356_23420 at 5251.698 kb on - strand, within AO356_23420 at 5251.843 kb on + strand, within AO356_23420 at 5251.844 kb on - strand, within AO356_23420 at 5251.844 kb on - strand, within AO356_23420 at 5251.844 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.946 kb on - strand, within AO356_23420 at 5251.946 kb on - strand, within AO356_23420 at 5251.998 kb on - strand, within AO356_23420 at 5252.046 kb on - strand, within AO356_23420 at 5252.046 kb on - strand, within AO356_23420 at 5252.130 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.289 kb on - strand, within AO356_23420 at 5252.494 kb on + strand at 5252.494 kb on + strand at 5252.515 kb on + strand at 5252.516 kb on - strand at 5252.519 kb on + strand at 5252.519 kb on + strand at 5252.519 kb on + strand at 5252.520 kb on - strand at 5252.520 kb on - strand at 5252.591 kb on + strand at 5252.592 kb on - strand at 5252.784 kb on - strand, within AO356_23425 at 5252.784 kb on - strand, within AO356_23425 at 5252.784 kb on - strand, within AO356_23425 at 5252.889 kb on + strand, within AO356_23425 at 5252.890 kb on - strand, within AO356_23425 at 5253.207 kb on + strand, within AO356_23425 at 5253.208 kb on - strand, within AO356_23425 at 5253.294 kb on + strand, within AO356_23425 at 5253.294 kb on + strand, within AO356_23425 at 5253.295 kb on - strand, within AO356_23425 at 5253.295 kb on - strand, within AO356_23425 at 5253.504 kb on + strand, within AO356_23425 at 5253.837 kb on - strand at 5253.918 kb on + strand at 5253.918 kb on + strand at 5253.919 kb on - strand at 5253.985 kb on - strand at 5254.041 kb on - strand at 5254.165 kb on + strand at 5254.165 kb on + strand at 5254.165 kb on + strand at 5254.165 kb on + strand at 5254.166 kb on - strand at 5254.166 kb on - strand at 5254.166 kb on - strand at 5254.166 kb on - strand at 5254.217 kb on + strand, within AO356_23430 at 5254.218 kb on - strand, within AO356_23430 at 5254.223 kb on + strand, within AO356_23430 at 5254.223 kb on + strand, within AO356_23430 at 5254.223 kb on + strand, within AO356_23430 at 5254.224 kb on - strand, within AO356_23430 at 5254.224 kb on - strand, within AO356_23430 at 5254.678 kb on + strand, within AO356_23430 at 5254.678 kb on + strand, within AO356_23430 at 5254.678 kb on + strand, within AO356_23430 at 5254.679 kb on - strand, within AO356_23430
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,251,529 + AO356_23420 0.14 -0.6 5,251,529 + AO356_23420 0.14 -0.6 5,251,530 - AO356_23420 0.14 +0.6 5,251,530 - AO356_23420 0.14 +0.2 5,251,530 - AO356_23420 0.14 -0.6 5,251,530 - AO356_23420 0.14 -1.3 5,251,530 - AO356_23420 0.14 +0.6 5,251,607 + AO356_23420 0.21 -0.6 5,251,698 - AO356_23420 0.29 +0.2 5,251,843 + AO356_23420 0.42 -0.6 5,251,844 - AO356_23420 0.42 -0.1 5,251,844 - AO356_23420 0.42 +0.8 5,251,844 - AO356_23420 0.42 -0.1 5,251,939 - AO356_23420 0.51 +0.7 5,251,939 - AO356_23420 0.51 +0.3 5,251,939 - AO356_23420 0.51 +0.5 5,251,939 - AO356_23420 0.51 -0.4 5,251,939 - AO356_23420 0.51 +0.1 5,251,939 - AO356_23420 0.51 +1.3 5,251,939 - AO356_23420 0.51 -0.4 5,251,939 - AO356_23420 0.51 +0.2 5,251,946 - AO356_23420 0.52 +0.4 5,251,946 - AO356_23420 0.52 -1.6 5,251,998 - AO356_23420 0.56 +1.0 5,252,046 - AO356_23420 0.61 +0.7 5,252,046 - AO356_23420 0.61 -0.8 5,252,130 - AO356_23420 0.68 -0.2 5,252,241 - AO356_23420 0.79 +0.0 5,252,241 - AO356_23420 0.79 -0.4 5,252,241 - AO356_23420 0.79 -0.4 5,252,241 - AO356_23420 0.79 +0.4 5,252,241 - AO356_23420 0.79 +1.4 5,252,289 - AO356_23420 0.83 +0.2 5,252,494 + +0.0 5,252,494 + -0.1 5,252,515 + -1.2 5,252,516 - +1.1 5,252,519 + +1.8 5,252,519 + +2.2 5,252,519 + +0.8 5,252,520 - +1.8 5,252,520 - +0.1 5,252,591 + +1.6 5,252,592 - -1.1 5,252,784 - AO356_23425 0.23 +0.3 5,252,784 - AO356_23425 0.23 +0.2 5,252,784 - AO356_23425 0.23 +1.2 5,252,889 + AO356_23425 0.31 -1.0 5,252,890 - AO356_23425 0.31 +0.4 5,253,207 + AO356_23425 0.57 +0.2 5,253,208 - AO356_23425 0.57 +2.4 5,253,294 + AO356_23425 0.64 +0.8 5,253,294 + AO356_23425 0.64 -0.1 5,253,295 - AO356_23425 0.64 -0.3 5,253,295 - AO356_23425 0.64 -0.1 5,253,504 + AO356_23425 0.80 +0.4 5,253,837 - -0.6 5,253,918 + +0.2 5,253,918 + -0.2 5,253,919 - -0.4 5,253,985 - -0.0 5,254,041 - -0.6 5,254,165 + +0.2 5,254,165 + -0.2 5,254,165 + -0.4 5,254,165 + +0.6 5,254,166 - +0.4 5,254,166 - -0.8 5,254,166 - +1.1 5,254,166 - +0.1 5,254,217 + AO356_23430 0.11 -0.4 5,254,218 - AO356_23430 0.12 +0.1 5,254,223 + AO356_23430 0.12 +0.6 5,254,223 + AO356_23430 0.12 +0.1 5,254,223 + AO356_23430 0.12 +0.1 5,254,224 - AO356_23430 0.12 -1.6 5,254,224 - AO356_23430 0.12 +0.1 5,254,678 + AO356_23430 0.72 +0.2 5,254,678 + AO356_23430 0.72 +0.4 5,254,678 + AO356_23430 0.72 +1.0 5,254,679 - AO356_23430 0.72 +1.0
Or see this region's nucleotide sequence