Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23415 and AO356_23420 are separated by 251 nucleotides AO356_23420 and AO356_23425 are separated by 25 nucleotides
AO356_23415: AO356_23415 - phosphatase, at 5,250,192 to 5,251,127
_23415
AO356_23420: AO356_23420 - carboxynorspermidine decarboxylase, at 5,251,379 to 5,252,476
_23420
AO356_23425: AO356_23425 - saccharopine dehydrogenase, at 5,252,502 to 5,253,746
_23425
Position (kb) 5251
5252
5253 Strain fitness (log2 ratio) -1
0
1
2 at 5250.562 kb on - strand, within AO356_23415 at 5250.909 kb on - strand, within AO356_23415 at 5250.909 kb on - strand, within AO356_23415 at 5250.909 kb on - strand, within AO356_23415 at 5250.912 kb on + strand, within AO356_23415 at 5250.912 kb on + strand, within AO356_23415 at 5250.913 kb on - strand, within AO356_23415 at 5251.118 kb on + strand at 5251.118 kb on + strand at 5251.119 kb on - strand at 5251.198 kb on + strand at 5251.198 kb on + strand at 5251.244 kb on + strand at 5251.244 kb on + strand at 5251.244 kb on + strand at 5251.245 kb on - strand at 5251.245 kb on - strand at 5251.394 kb on + strand at 5251.395 kb on - strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.398 kb on - strand at 5251.398 kb on - strand at 5251.529 kb on + strand, within AO356_23420 at 5251.529 kb on + strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.530 kb on - strand, within AO356_23420 at 5251.607 kb on + strand, within AO356_23420 at 5251.698 kb on - strand, within AO356_23420 at 5251.843 kb on + strand, within AO356_23420 at 5251.844 kb on - strand, within AO356_23420 at 5251.844 kb on - strand, within AO356_23420 at 5251.844 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.939 kb on - strand, within AO356_23420 at 5251.946 kb on - strand, within AO356_23420 at 5251.946 kb on - strand, within AO356_23420 at 5251.998 kb on - strand, within AO356_23420 at 5252.046 kb on - strand, within AO356_23420 at 5252.046 kb on - strand, within AO356_23420 at 5252.130 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.241 kb on - strand, within AO356_23420 at 5252.289 kb on - strand, within AO356_23420 at 5252.494 kb on + strand at 5252.494 kb on + strand at 5252.515 kb on + strand at 5252.516 kb on - strand at 5252.519 kb on + strand at 5252.519 kb on + strand at 5252.519 kb on + strand at 5252.520 kb on - strand at 5252.520 kb on - strand at 5252.591 kb on + strand at 5252.592 kb on - strand at 5252.784 kb on - strand, within AO356_23425 at 5252.784 kb on - strand, within AO356_23425 at 5252.784 kb on - strand, within AO356_23425 at 5252.889 kb on + strand, within AO356_23425 at 5252.890 kb on - strand, within AO356_23425 at 5253.207 kb on + strand, within AO356_23425 at 5253.208 kb on - strand, within AO356_23425 at 5253.294 kb on + strand, within AO356_23425 at 5253.294 kb on + strand, within AO356_23425 at 5253.295 kb on - strand, within AO356_23425 at 5253.295 kb on - strand, within AO356_23425
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,250,562 - AO356_23415 0.40 -0.5 5,250,909 - AO356_23415 0.77 +0.4 5,250,909 - AO356_23415 0.77 +0.9 5,250,909 - AO356_23415 0.77 -1.1 5,250,912 + AO356_23415 0.77 -0.1 5,250,912 + AO356_23415 0.77 +0.2 5,250,913 - AO356_23415 0.77 -0.0 5,251,118 + -0.6 5,251,118 + +0.3 5,251,119 - +0.5 5,251,198 + -0.0 5,251,198 + -0.4 5,251,244 + -0.4 5,251,244 + +0.3 5,251,244 + +0.0 5,251,245 - +0.1 5,251,245 - -0.6 5,251,394 + +1.0 5,251,395 - +0.7 5,251,397 + -1.6 5,251,397 + +0.2 5,251,397 + +0.0 5,251,397 + -1.6 5,251,398 - -0.4 5,251,398 - +0.6 5,251,529 + AO356_23420 0.14 -0.6 5,251,529 + AO356_23420 0.14 -0.6 5,251,530 - AO356_23420 0.14 +0.6 5,251,530 - AO356_23420 0.14 +0.2 5,251,530 - AO356_23420 0.14 -0.6 5,251,530 - AO356_23420 0.14 -1.3 5,251,530 - AO356_23420 0.14 +0.6 5,251,607 + AO356_23420 0.21 -0.6 5,251,698 - AO356_23420 0.29 +0.2 5,251,843 + AO356_23420 0.42 -0.6 5,251,844 - AO356_23420 0.42 -0.1 5,251,844 - AO356_23420 0.42 +0.8 5,251,844 - AO356_23420 0.42 -0.1 5,251,939 - AO356_23420 0.51 +0.7 5,251,939 - AO356_23420 0.51 +0.3 5,251,939 - AO356_23420 0.51 +0.5 5,251,939 - AO356_23420 0.51 -0.4 5,251,939 - AO356_23420 0.51 +0.1 5,251,939 - AO356_23420 0.51 +1.3 5,251,939 - AO356_23420 0.51 -0.4 5,251,939 - AO356_23420 0.51 +0.2 5,251,946 - AO356_23420 0.52 +0.4 5,251,946 - AO356_23420 0.52 -1.6 5,251,998 - AO356_23420 0.56 +1.0 5,252,046 - AO356_23420 0.61 +0.7 5,252,046 - AO356_23420 0.61 -0.8 5,252,130 - AO356_23420 0.68 -0.2 5,252,241 - AO356_23420 0.79 +0.0 5,252,241 - AO356_23420 0.79 -0.4 5,252,241 - AO356_23420 0.79 -0.4 5,252,241 - AO356_23420 0.79 +0.4 5,252,241 - AO356_23420 0.79 +1.4 5,252,289 - AO356_23420 0.83 +0.2 5,252,494 + +0.0 5,252,494 + -0.1 5,252,515 + -1.2 5,252,516 - +1.1 5,252,519 + +1.8 5,252,519 + +2.2 5,252,519 + +0.8 5,252,520 - +1.8 5,252,520 - +0.1 5,252,591 + +1.6 5,252,592 - -1.1 5,252,784 - AO356_23425 0.23 +0.3 5,252,784 - AO356_23425 0.23 +0.2 5,252,784 - AO356_23425 0.23 +1.2 5,252,889 + AO356_23425 0.31 -1.0 5,252,890 - AO356_23425 0.31 +0.4 5,253,207 + AO356_23425 0.57 +0.2 5,253,208 - AO356_23425 0.57 +2.4 5,253,294 + AO356_23425 0.64 +0.8 5,253,294 + AO356_23425 0.64 -0.1 5,253,295 - AO356_23425 0.64 -0.3 5,253,295 - AO356_23425 0.64 -0.1
Or see this region's nucleotide sequence