Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_20140 and AO356_20145 are separated by 105 nucleotides AO356_20145 and AO356_20150 are separated by 13 nucleotides AO356_20150 and AO356_20155 are separated by 34 nucleotides AO356_20155 and AO356_20160 are separated by 123 nucleotides
AO356_20140: AO356_20140 - septum formation inhibitor Maf, at 4,507,992 to 4,508,570
_20140
AO356_20145: AO356_20145 - metal-binding protein, at 4,508,676 to 4,509,203
_20145
AO356_20150: AO356_20150 - 50S ribosomal protein L32, at 4,509,217 to 4,509,399
_20150
AO356_20155: AO356_20155 - phosphate acyltransferase, at 4,509,434 to 4,510,414
_20155
AO356_20160: AO356_20160 - malonyl CoA-ACP transacylase, at 4,510,538 to 4,511,476
_20160
Position (kb)
4509
4510
4511 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4508.536 kb on + strand at 4508.537 kb on - strand at 4508.556 kb on + strand at 4508.556 kb on + strand at 4508.556 kb on + strand at 4508.556 kb on + strand at 4508.556 kb on + strand at 4508.556 kb on + strand at 4508.557 kb on - strand at 4508.557 kb on - strand at 4508.557 kb on - strand at 4508.557 kb on - strand at 4508.559 kb on + strand at 4508.559 kb on + strand at 4508.559 kb on + strand at 4508.559 kb on + strand at 4508.560 kb on - strand at 4509.493 kb on + strand at 4509.494 kb on - strand at 4509.752 kb on + strand, within AO356_20155 at 4509.753 kb on - strand, within AO356_20155 at 4509.753 kb on - strand, within AO356_20155 at 4510.028 kb on + strand, within AO356_20155 at 4510.028 kb on + strand, within AO356_20155 at 4510.029 kb on - strand, within AO356_20155 at 4510.029 kb on - strand, within AO356_20155 at 4510.029 kb on - strand, within AO356_20155 at 4510.058 kb on + strand, within AO356_20155 at 4510.058 kb on + strand, within AO356_20155 at 4510.105 kb on + strand, within AO356_20155 at 4510.285 kb on + strand, within AO356_20155 at 4510.285 kb on + strand, within AO356_20155 at 4510.285 kb on + strand, within AO356_20155 at 4510.286 kb on + strand, within AO356_20155 at 4510.286 kb on - strand, within AO356_20155 at 4510.412 kb on + strand at 4510.436 kb on + strand at 4510.436 kb on + strand at 4510.436 kb on + strand at 4510.436 kb on + strand at 4510.436 kb on + strand at 4510.437 kb on - strand at 4510.437 kb on - strand at 4510.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 4,508,536 + -0.1 4,508,537 - +1.0 4,508,556 + +0.2 4,508,556 + +0.7 4,508,556 + -1.8 4,508,556 + +0.7 4,508,556 + +0.8 4,508,556 + -0.8 4,508,557 - -0.3 4,508,557 - +0.7 4,508,557 - +0.7 4,508,557 - -0.3 4,508,559 + +0.1 4,508,559 + -0.1 4,508,559 + +0.3 4,508,559 + +1.4 4,508,560 - +0.7 4,509,493 + -3.1 4,509,494 - -1.9 4,509,752 + AO356_20155 0.32 -2.9 4,509,753 - AO356_20155 0.33 +0.1 4,509,753 - AO356_20155 0.33 -1.9 4,510,028 + AO356_20155 0.61 -2.3 4,510,028 + AO356_20155 0.61 -1.3 4,510,029 - AO356_20155 0.61 -0.1 4,510,029 - AO356_20155 0.61 -1.1 4,510,029 - AO356_20155 0.61 -0.3 4,510,058 + AO356_20155 0.64 +0.3 4,510,058 + AO356_20155 0.64 -0.9 4,510,105 + AO356_20155 0.68 -1.3 4,510,285 + AO356_20155 0.87 -1.3 4,510,285 + AO356_20155 0.87 -1.7 4,510,285 + AO356_20155 0.87 -1.9 4,510,286 + AO356_20155 0.87 -3.5 4,510,286 - AO356_20155 0.87 -1.6 4,510,412 + -0.8 4,510,436 + -1.1 4,510,436 + +1.0 4,510,436 + -0.3 4,510,436 + +0.7 4,510,436 + +0.1 4,510,437 - -0.3 4,510,437 - -0.3 4,510,437 - -2.7
Or see this region's nucleotide sequence