Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_17760 and AO356_17765 are separated by 327 nucleotides AO356_17765 and AO356_17770 are separated by 43 nucleotides AO356_17770 and AO356_17775 are separated by 174 nucleotides AO356_17775 and AO356_17780 are separated by 3 nucleotides
AO356_17760: AO356_17760 - AraC family transcriptional regulator, at 4,008,484 to 4,009,413
_17760
AO356_17765: AO356_17765 - antibiotic biosynthesis monooxygenase, at 4,009,741 to 4,010,094
_17765
AO356_17770: AO356_17770 - hypothetical protein, at 4,010,138 to 4,010,371
_17770
AO356_17775: AO356_17775 - Asp/Glu/hydantoin racemase, at 4,010,546 to 4,011,166
_17775
AO356_17780: AO356_17780 - hypothetical protein, at 4,011,170 to 4,011,406
_17780
Position (kb)
4010
4011 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4009.155 kb on - strand, within AO356_17760 at 4009.155 kb on - strand, within AO356_17760 at 4009.169 kb on + strand, within AO356_17760 at 4009.169 kb on + strand, within AO356_17760 at 4009.169 kb on + strand, within AO356_17760 at 4009.170 kb on - strand, within AO356_17760 at 4009.170 kb on - strand, within AO356_17760 at 4009.170 kb on - strand, within AO356_17760 at 4009.307 kb on + strand, within AO356_17760 at 4009.308 kb on - strand, within AO356_17760 at 4009.308 kb on - strand, within AO356_17760 at 4009.308 kb on - strand, within AO356_17760 at 4009.373 kb on + strand at 4009.373 kb on + strand at 4009.373 kb on + strand at 4009.373 kb on + strand at 4009.373 kb on + strand at 4009.373 kb on + strand at 4009.373 kb on + strand at 4009.374 kb on - strand at 4009.374 kb on - strand at 4009.374 kb on - strand at 4009.374 kb on - strand at 4009.374 kb on - strand at 4009.453 kb on + strand at 4009.453 kb on + strand at 4009.453 kb on + strand at 4009.453 kb on + strand at 4009.453 kb on + strand at 4009.453 kb on + strand at 4009.454 kb on - strand at 4009.454 kb on - strand at 4009.454 kb on - strand at 4009.454 kb on - strand at 4009.454 kb on - strand at 4009.454 kb on - strand at 4009.454 kb on - strand at 4009.454 kb on - strand at 4009.678 kb on + strand at 4009.678 kb on + strand at 4009.724 kb on - strand at 4009.746 kb on + strand at 4009.746 kb on + strand at 4009.746 kb on + strand at 4009.746 kb on + strand at 4009.746 kb on + strand at 4009.747 kb on - strand at 4009.747 kb on - strand at 4009.747 kb on - strand at 4009.747 kb on - strand at 4009.825 kb on - strand, within AO356_17765 at 4009.948 kb on + strand, within AO356_17765 at 4009.949 kb on - strand, within AO356_17765 at 4010.075 kb on + strand at 4010.075 kb on + strand at 4010.100 kb on + strand at 4010.101 kb on - strand at 4010.146 kb on + strand at 4010.147 kb on - strand at 4010.147 kb on - strand at 4010.147 kb on - strand at 4010.147 kb on - strand at 4010.204 kb on + strand, within AO356_17770 at 4010.369 kb on + strand at 4010.398 kb on + strand at 4010.398 kb on + strand at 4010.398 kb on + strand at 4010.399 kb on - strand at 4010.399 kb on - strand at 4010.399 kb on - strand at 4010.419 kb on + strand at 4010.419 kb on + strand at 4010.420 kb on - strand at 4010.511 kb on - strand at 4010.547 kb on + strand at 4010.548 kb on - strand at 4010.548 kb on - strand at 4010.548 kb on - strand at 4010.548 kb on - strand at 4010.646 kb on + strand, within AO356_17775 at 4010.646 kb on + strand, within AO356_17775 at 4010.646 kb on + strand, within AO356_17775 at 4010.646 kb on + strand, within AO356_17775 at 4010.646 kb on + strand, within AO356_17775 at 4010.647 kb on - strand, within AO356_17775 at 4010.647 kb on - strand, within AO356_17775 at 4010.647 kb on - strand, within AO356_17775 at 4010.647 kb on - strand, within AO356_17775 at 4010.647 kb on - strand, within AO356_17775 at 4011.003 kb on + strand, within AO356_17775 at 4011.003 kb on + strand, within AO356_17775 at 4011.003 kb on + strand, within AO356_17775 at 4011.003 kb on + strand, within AO356_17775 at 4011.004 kb on - strand, within AO356_17775 at 4011.004 kb on - strand, within AO356_17775 at 4011.004 kb on - strand, within AO356_17775 at 4011.027 kb on + strand, within AO356_17775 at 4011.028 kb on - strand, within AO356_17775 at 4011.144 kb on + strand at 4011.144 kb on + strand at 4011.144 kb on + strand at 4011.145 kb on - strand at 4011.145 kb on - strand at 4011.145 kb on - strand at 4011.145 kb on - strand at 4011.145 kb on - strand at 4011.147 kb on + strand at 4011.147 kb on + strand at 4011.148 kb on - strand at 4011.148 kb on - strand at 4011.148 kb on - strand at 4011.207 kb on + strand, within AO356_17780 at 4011.208 kb on - strand, within AO356_17780 at 4011.288 kb on + strand, within AO356_17780 at 4011.360 kb on + strand, within AO356_17780 at 4011.360 kb on + strand, within AO356_17780 at 4011.360 kb on + strand, within AO356_17780 at 4011.360 kb on + strand, within AO356_17780 at 4011.360 kb on + strand, within AO356_17780 at 4011.360 kb on + strand, within AO356_17780 at 4011.361 kb on - strand, within AO356_17780 at 4011.361 kb on - strand, within AO356_17780 at 4011.361 kb on - strand, within AO356_17780 at 4011.361 kb on - strand, within AO356_17780
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 4,009,155 - AO356_17760 0.72 -0.1 4,009,155 - AO356_17760 0.72 -0.4 4,009,169 + AO356_17760 0.74 +0.0 4,009,169 + AO356_17760 0.74 +0.3 4,009,169 + AO356_17760 0.74 +0.1 4,009,170 - AO356_17760 0.74 -0.8 4,009,170 - AO356_17760 0.74 -0.2 4,009,170 - AO356_17760 0.74 -1.5 4,009,307 + AO356_17760 0.88 +0.6 4,009,308 - AO356_17760 0.89 +0.3 4,009,308 - AO356_17760 0.89 -0.4 4,009,308 - AO356_17760 0.89 +0.8 4,009,373 + +0.5 4,009,373 + -0.8 4,009,373 + -0.5 4,009,373 + -1.1 4,009,373 + +0.9 4,009,373 + +0.7 4,009,373 + +0.3 4,009,374 - +0.3 4,009,374 - +0.6 4,009,374 - -0.2 4,009,374 - -0.0 4,009,374 - +0.4 4,009,453 + -0.5 4,009,453 + -0.0 4,009,453 + -1.8 4,009,453 + -1.8 4,009,453 + +0.8 4,009,453 + +0.2 4,009,454 - +0.4 4,009,454 - -0.4 4,009,454 - +1.8 4,009,454 - -0.4 4,009,454 - +2.4 4,009,454 - -0.5 4,009,454 - -0.1 4,009,454 - +0.7 4,009,678 + -0.1 4,009,678 + +1.4 4,009,724 - +0.8 4,009,746 + -0.9 4,009,746 + +0.2 4,009,746 + +0.5 4,009,746 + -0.4 4,009,746 + +0.6 4,009,747 - +0.0 4,009,747 - -2.2 4,009,747 - +1.0 4,009,747 - -0.0 4,009,825 - AO356_17765 0.24 +0.2 4,009,948 + AO356_17765 0.58 +0.4 4,009,949 - AO356_17765 0.59 +0.7 4,010,075 + +0.4 4,010,075 + -0.0 4,010,100 + -1.5 4,010,101 - -0.0 4,010,146 + +0.3 4,010,147 - +0.6 4,010,147 - -0.8 4,010,147 - +0.3 4,010,147 - -0.2 4,010,204 + AO356_17770 0.28 -0.6 4,010,369 + -0.0 4,010,398 + +0.4 4,010,398 + +0.1 4,010,398 + +0.4 4,010,399 - -1.9 4,010,399 - +1.0 4,010,399 - +0.7 4,010,419 + -0.6 4,010,419 + -0.0 4,010,420 - +0.2 4,010,511 - +0.1 4,010,547 + +1.1 4,010,548 - -1.1 4,010,548 - +0.3 4,010,548 - -0.7 4,010,548 - +0.1 4,010,646 + AO356_17775 0.16 +0.3 4,010,646 + AO356_17775 0.16 +0.6 4,010,646 + AO356_17775 0.16 +1.0 4,010,646 + AO356_17775 0.16 -0.9 4,010,646 + AO356_17775 0.16 +0.3 4,010,647 - AO356_17775 0.16 -0.0 4,010,647 - AO356_17775 0.16 -1.3 4,010,647 - AO356_17775 0.16 +0.4 4,010,647 - AO356_17775 0.16 +0.2 4,010,647 - AO356_17775 0.16 +0.4 4,011,003 + AO356_17775 0.74 +0.2 4,011,003 + AO356_17775 0.74 +0.1 4,011,003 + AO356_17775 0.74 +0.5 4,011,003 + AO356_17775 0.74 -1.2 4,011,004 - AO356_17775 0.74 -0.4 4,011,004 - AO356_17775 0.74 +0.0 4,011,004 - AO356_17775 0.74 +0.4 4,011,027 + AO356_17775 0.77 +0.2 4,011,028 - AO356_17775 0.78 -0.2 4,011,144 + +0.3 4,011,144 + +0.4 4,011,144 + -1.9 4,011,145 - +1.0 4,011,145 - +1.3 4,011,145 - +0.1 4,011,145 - +1.4 4,011,145 - -0.1 4,011,147 + +0.2 4,011,147 + -0.5 4,011,148 - +0.5 4,011,148 - +0.3 4,011,148 - -0.0 4,011,207 + AO356_17780 0.16 -1.7 4,011,208 - AO356_17780 0.16 -0.9 4,011,288 + AO356_17780 0.50 -0.2 4,011,360 + AO356_17780 0.80 -0.7 4,011,360 + AO356_17780 0.80 -0.5 4,011,360 + AO356_17780 0.80 +2.1 4,011,360 + AO356_17780 0.80 -0.2 4,011,360 + AO356_17780 0.80 -0.5 4,011,360 + AO356_17780 0.80 +0.2 4,011,361 - AO356_17780 0.81 +0.2 4,011,361 - AO356_17780 0.81 +0.1 4,011,361 - AO356_17780 0.81 +0.9 4,011,361 - AO356_17780 0.81 +0.1
Or see this region's nucleotide sequence