Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_14435 and AO356_14440 overlap by 4 nucleotides AO356_14440 and AO356_14445 are separated by 3 nucleotides AO356_14445 and AO356_14450 overlap by 4 nucleotides
AO356_14435: AO356_14435 - anthranilate phosphoribosyltransferase, at 3,284,551 to 3,285,600
_14435
AO356_14440: AO356_14440 - indole-3-glycerol-phosphate synthase, at 3,285,597 to 3,286,433
_14440
AO356_14445: AO356_14445 - lipoate--protein ligase, at 3,286,437 to 3,287,138
_14445
AO356_14450: AO356_14450 - cyclic AMP receptor protein, at 3,287,135 to 3,287,779
_14450
Position (kb)
3285
3286
3287 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3284.817 kb on + strand, within AO356_14435 at 3284.817 kb on + strand, within AO356_14435 at 3284.944 kb on + strand, within AO356_14435 at 3284.944 kb on + strand, within AO356_14435 at 3284.944 kb on + strand, within AO356_14435 at 3284.945 kb on - strand, within AO356_14435 at 3284.945 kb on - strand, within AO356_14435 at 3284.945 kb on - strand, within AO356_14435 at 3285.072 kb on + strand, within AO356_14435 at 3285.073 kb on - strand, within AO356_14435 at 3285.099 kb on + strand, within AO356_14435 at 3285.270 kb on - strand, within AO356_14435 at 3285.292 kb on + strand, within AO356_14435 at 3285.292 kb on + strand, within AO356_14435 at 3285.293 kb on - strand, within AO356_14435 at 3285.293 kb on - strand, within AO356_14435 at 3285.463 kb on + strand, within AO356_14435 at 3285.463 kb on + strand, within AO356_14435 at 3285.463 kb on + strand, within AO356_14435 at 3285.463 kb on + strand, within AO356_14435 at 3285.464 kb on - strand, within AO356_14435 at 3285.568 kb on - strand at 3285.576 kb on - strand at 3285.849 kb on + strand, within AO356_14440 at 3285.850 kb on - strand, within AO356_14440 at 3285.918 kb on + strand, within AO356_14440 at 3286.320 kb on + strand, within AO356_14440 at 3286.320 kb on + strand, within AO356_14440 at 3286.321 kb on - strand, within AO356_14440 at 3286.615 kb on + strand, within AO356_14445 at 3286.707 kb on + strand, within AO356_14445 at 3286.707 kb on + strand, within AO356_14445 at 3286.708 kb on - strand, within AO356_14445 at 3287.104 kb on - strand at 3287.386 kb on + strand, within AO356_14450 at 3287.386 kb on + strand, within AO356_14450 at 3287.387 kb on - strand, within AO356_14450 at 3287.424 kb on + strand, within AO356_14450 at 3287.424 kb on + strand, within AO356_14450 at 3287.424 kb on + strand, within AO356_14450 at 3287.424 kb on + strand, within AO356_14450 at 3287.425 kb on - strand, within AO356_14450 at 3287.425 kb on - strand, within AO356_14450 at 3287.425 kb on - strand, within AO356_14450
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,284,817 + AO356_14435 0.25 -2.0 3,284,817 + AO356_14435 0.25 -1.6 3,284,944 + AO356_14435 0.37 -2.8 3,284,944 + AO356_14435 0.37 -1.4 3,284,944 + AO356_14435 0.37 -0.8 3,284,945 - AO356_14435 0.38 -3.0 3,284,945 - AO356_14435 0.38 -3.2 3,284,945 - AO356_14435 0.38 -3.6 3,285,072 + AO356_14435 0.50 -2.2 3,285,073 - AO356_14435 0.50 -2.6 3,285,099 + AO356_14435 0.52 -3.5 3,285,270 - AO356_14435 0.68 -2.7 3,285,292 + AO356_14435 0.71 -2.2 3,285,292 + AO356_14435 0.71 -3.6 3,285,293 - AO356_14435 0.71 -1.6 3,285,293 - AO356_14435 0.71 -1.8 3,285,463 + AO356_14435 0.87 -2.8 3,285,463 + AO356_14435 0.87 -1.6 3,285,463 + AO356_14435 0.87 -1.5 3,285,463 + AO356_14435 0.87 -1.6 3,285,464 - AO356_14435 0.87 -1.6 3,285,568 - -2.2 3,285,576 - -2.5 3,285,849 + AO356_14440 0.30 -3.0 3,285,850 - AO356_14440 0.30 -2.8 3,285,918 + AO356_14440 0.38 -2.7 3,286,320 + AO356_14440 0.86 -2.0 3,286,320 + AO356_14440 0.86 -2.6 3,286,321 - AO356_14440 0.86 -0.8 3,286,615 + AO356_14445 0.25 +0.5 3,286,707 + AO356_14445 0.38 +0.6 3,286,707 + AO356_14445 0.38 +0.6 3,286,708 - AO356_14445 0.39 +0.3 3,287,104 - -0.7 3,287,386 + AO356_14450 0.39 +0.5 3,287,386 + AO356_14450 0.39 +0.8 3,287,387 - AO356_14450 0.39 -0.1 3,287,424 + AO356_14450 0.45 +1.4 3,287,424 + AO356_14450 0.45 -0.3 3,287,424 + AO356_14450 0.45 +1.3 3,287,424 + AO356_14450 0.45 +0.2 3,287,425 - AO356_14450 0.45 -0.0 3,287,425 - AO356_14450 0.45 +0.8 3,287,425 - AO356_14450 0.45 -1.0
Or see this region's nucleotide sequence