Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_14440

Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_14435 and AO356_14440 overlap by 4 nucleotidesAO356_14440 and AO356_14445 are separated by 3 nucleotidesAO356_14445 and AO356_14450 overlap by 4 nucleotides AO356_14435: AO356_14435 - anthranilate phosphoribosyltransferase, at 3,284,551 to 3,285,600 _14435 AO356_14440: AO356_14440 - indole-3-glycerol-phosphate synthase, at 3,285,597 to 3,286,433 _14440 AO356_14445: AO356_14445 - lipoate--protein ligase, at 3,286,437 to 3,287,138 _14445 AO356_14450: AO356_14450 - cyclic AMP receptor protein, at 3,287,135 to 3,287,779 _14450 Position (kb) 3285 3286 3287Strain fitness (log2 ratio) -3 -2 -1 0 1at 3284.817 kb on + strand, within AO356_14435at 3284.817 kb on + strand, within AO356_14435at 3284.944 kb on + strand, within AO356_14435at 3284.944 kb on + strand, within AO356_14435at 3284.944 kb on + strand, within AO356_14435at 3284.945 kb on - strand, within AO356_14435at 3284.945 kb on - strand, within AO356_14435at 3284.945 kb on - strand, within AO356_14435at 3285.072 kb on + strand, within AO356_14435at 3285.073 kb on - strand, within AO356_14435at 3285.099 kb on + strand, within AO356_14435at 3285.270 kb on - strand, within AO356_14435at 3285.292 kb on + strand, within AO356_14435at 3285.292 kb on + strand, within AO356_14435at 3285.293 kb on - strand, within AO356_14435at 3285.293 kb on - strand, within AO356_14435at 3285.463 kb on + strand, within AO356_14435at 3285.463 kb on + strand, within AO356_14435at 3285.463 kb on + strand, within AO356_14435at 3285.463 kb on + strand, within AO356_14435at 3285.464 kb on - strand, within AO356_14435at 3285.568 kb on - strandat 3285.576 kb on - strandat 3285.849 kb on + strand, within AO356_14440at 3285.850 kb on - strand, within AO356_14440at 3285.918 kb on + strand, within AO356_14440at 3286.320 kb on + strand, within AO356_14440at 3286.320 kb on + strand, within AO356_14440at 3286.321 kb on - strand, within AO356_14440at 3286.615 kb on + strand, within AO356_14445at 3286.707 kb on + strand, within AO356_14445at 3286.707 kb on + strand, within AO356_14445at 3286.708 kb on - strand, within AO356_14445at 3287.104 kb on - strandat 3287.386 kb on + strand, within AO356_14450at 3287.386 kb on + strand, within AO356_14450at 3287.387 kb on - strand, within AO356_14450at 3287.424 kb on + strand, within AO356_14450at 3287.424 kb on + strand, within AO356_14450at 3287.424 kb on + strand, within AO356_14450at 3287.424 kb on + strand, within AO356_14450at 3287.425 kb on - strand, within AO356_14450at 3287.425 kb on - strand, within AO356_14450at 3287.425 kb on - strand, within AO356_14450

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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3,284,817 + AO356_14435 0.25 -2.0
3,284,817 + AO356_14435 0.25 -1.6
3,284,944 + AO356_14435 0.37 -2.8
3,284,944 + AO356_14435 0.37 -1.4
3,284,944 + AO356_14435 0.37 -0.8
3,284,945 - AO356_14435 0.38 -3.0
3,284,945 - AO356_14435 0.38 -3.2
3,284,945 - AO356_14435 0.38 -3.6
3,285,072 + AO356_14435 0.50 -2.2
3,285,073 - AO356_14435 0.50 -2.6
3,285,099 + AO356_14435 0.52 -3.5
3,285,270 - AO356_14435 0.68 -2.7
3,285,292 + AO356_14435 0.71 -2.2
3,285,292 + AO356_14435 0.71 -3.6
3,285,293 - AO356_14435 0.71 -1.6
3,285,293 - AO356_14435 0.71 -1.8
3,285,463 + AO356_14435 0.87 -2.8
3,285,463 + AO356_14435 0.87 -1.6
3,285,463 + AO356_14435 0.87 -1.5
3,285,463 + AO356_14435 0.87 -1.6
3,285,464 - AO356_14435 0.87 -1.6
3,285,568 - -2.2
3,285,576 - -2.5
3,285,849 + AO356_14440 0.30 -3.0
3,285,850 - AO356_14440 0.30 -2.8
3,285,918 + AO356_14440 0.38 -2.7
3,286,320 + AO356_14440 0.86 -2.0
3,286,320 + AO356_14440 0.86 -2.6
3,286,321 - AO356_14440 0.86 -0.8
3,286,615 + AO356_14445 0.25 +0.5
3,286,707 + AO356_14445 0.38 +0.6
3,286,707 + AO356_14445 0.38 +0.6
3,286,708 - AO356_14445 0.39 +0.3
3,287,104 - -0.7
3,287,386 + AO356_14450 0.39 +0.5
3,287,386 + AO356_14450 0.39 +0.8
3,287,387 - AO356_14450 0.39 -0.1
3,287,424 + AO356_14450 0.45 +1.4
3,287,424 + AO356_14450 0.45 -0.3
3,287,424 + AO356_14450 0.45 +1.3
3,287,424 + AO356_14450 0.45 +0.2
3,287,425 - AO356_14450 0.45 -0.0
3,287,425 - AO356_14450 0.45 +0.8
3,287,425 - AO356_14450 0.45 -1.0

Or see this region's nucleotide sequence