Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_13475 and AO356_13480 are separated by 224 nucleotides AO356_13480 and AO356_13485 are separated by 48 nucleotides AO356_13485 and AO356_13490 are separated by 10 nucleotides
AO356_13475: AO356_13475 - glutathione synthetase, at 3,053,384 to 3,054,337
_13475
AO356_13480: AO356_13480 - pilus assembly protein PilG, at 3,054,562 to 3,054,990
_13480
AO356_13485: AO356_13485 - chemotaxis protein CheY, at 3,055,039 to 3,055,404
_13485
AO356_13490: AO356_13490 - protein PilI, at 3,055,415 to 3,055,954
_13490
Position (kb)
3054
3055 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3053.673 kb on + strand, within AO356_13475 at 3053.674 kb on - strand, within AO356_13475 at 3053.674 kb on - strand, within AO356_13475 at 3053.674 kb on - strand, within AO356_13475 at 3053.736 kb on + strand, within AO356_13475 at 3053.737 kb on - strand, within AO356_13475 at 3053.737 kb on - strand, within AO356_13475 at 3054.036 kb on + strand, within AO356_13475 at 3054.036 kb on + strand, within AO356_13475 at 3054.036 kb on + strand, within AO356_13475 at 3054.037 kb on - strand, within AO356_13475 at 3054.037 kb on - strand, within AO356_13475 at 3054.045 kb on + strand, within AO356_13475 at 3054.046 kb on - strand, within AO356_13475 at 3054.205 kb on - strand, within AO356_13475 at 3054.205 kb on - strand, within AO356_13475 at 3054.225 kb on + strand, within AO356_13475 at 3054.225 kb on + strand, within AO356_13475 at 3054.226 kb on - strand, within AO356_13475 at 3054.226 kb on - strand, within AO356_13475 at 3054.226 kb on - strand, within AO356_13475 at 3054.288 kb on + strand at 3054.289 kb on - strand at 3054.329 kb on + strand at 3054.330 kb on - strand at 3054.355 kb on + strand at 3054.355 kb on + strand at 3054.355 kb on + strand at 3054.355 kb on + strand at 3054.355 kb on + strand at 3054.355 kb on + strand at 3054.355 kb on + strand at 3054.356 kb on - strand at 3054.356 kb on - strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.368 kb on + strand at 3054.369 kb on - strand at 3054.369 kb on - strand at 3054.369 kb on - strand at 3054.369 kb on - strand at 3054.369 kb on - strand at 3054.369 kb on - strand at 3054.369 kb on - strand at 3054.369 kb on - strand at 3054.447 kb on + strand at 3054.447 kb on + strand at 3054.447 kb on + strand at 3054.448 kb on - strand at 3054.448 kb on - strand at 3054.448 kb on - strand at 3054.470 kb on + strand at 3054.470 kb on + strand at 3054.471 kb on - strand at 3054.576 kb on + strand at 3054.576 kb on + strand at 3054.576 kb on + strand at 3054.576 kb on + strand at 3054.775 kb on + strand, within AO356_13480 at 3054.938 kb on + strand, within AO356_13480 at 3054.938 kb on + strand, within AO356_13480 at 3054.988 kb on + strand at 3054.989 kb on - strand at 3054.989 kb on - strand at 3054.989 kb on - strand at 3055.081 kb on + strand, within AO356_13485 at 3055.082 kb on - strand, within AO356_13485 at 3055.082 kb on - strand, within AO356_13485 at 3055.082 kb on - strand, within AO356_13485 at 3055.194 kb on + strand, within AO356_13485 at 3055.339 kb on + strand, within AO356_13485 at 3055.553 kb on + strand, within AO356_13490 at 3055.553 kb on + strand, within AO356_13490 at 3055.553 kb on + strand, within AO356_13490 at 3055.553 kb on + strand, within AO356_13490 at 3055.554 kb on - strand, within AO356_13490 at 3055.554 kb on - strand, within AO356_13490 at 3055.554 kb on - strand, within AO356_13490 at 3055.554 kb on - strand, within AO356_13490 at 3055.554 kb on - strand, within AO356_13490 at 3055.554 kb on - strand, within AO356_13490
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 3,053,673 + AO356_13475 0.30 +0.6 3,053,674 - AO356_13475 0.30 +2.0 3,053,674 - AO356_13475 0.30 -0.4 3,053,674 - AO356_13475 0.30 +1.0 3,053,736 + AO356_13475 0.37 +0.0 3,053,737 - AO356_13475 0.37 +1.0 3,053,737 - AO356_13475 0.37 -1.5 3,054,036 + AO356_13475 0.68 +1.0 3,054,036 + AO356_13475 0.68 -0.4 3,054,036 + AO356_13475 0.68 +0.0 3,054,037 - AO356_13475 0.68 +0.0 3,054,037 - AO356_13475 0.68 -1.0 3,054,045 + AO356_13475 0.69 -1.0 3,054,046 - AO356_13475 0.69 +0.0 3,054,205 - AO356_13475 0.86 -2.3 3,054,205 - AO356_13475 0.86 +0.0 3,054,225 + AO356_13475 0.88 +0.6 3,054,225 + AO356_13475 0.88 +1.0 3,054,226 - AO356_13475 0.88 -0.7 3,054,226 - AO356_13475 0.88 -1.5 3,054,226 - AO356_13475 0.88 -0.3 3,054,288 + +1.0 3,054,289 - +1.0 3,054,329 + -2.0 3,054,330 - -0.2 3,054,355 + +0.0 3,054,355 + -0.4 3,054,355 + +1.6 3,054,355 + +0.9 3,054,355 + -1.2 3,054,355 + +0.6 3,054,355 + +0.3 3,054,356 - -0.4 3,054,356 - -0.3 3,054,368 + -1.0 3,054,368 + -0.4 3,054,368 + +0.2 3,054,368 + +0.5 3,054,368 + -0.6 3,054,368 + +1.4 3,054,368 + +0.6 3,054,368 + +0.6 3,054,368 + +0.5 3,054,368 + +0.4 3,054,369 - -0.4 3,054,369 - +0.3 3,054,369 - -0.3 3,054,369 - -0.4 3,054,369 - -0.0 3,054,369 - +0.1 3,054,369 - +0.1 3,054,369 - +0.4 3,054,447 + +0.0 3,054,447 + +1.6 3,054,447 + -0.1 3,054,448 - +0.4 3,054,448 - -0.7 3,054,448 - -0.5 3,054,470 + +0.8 3,054,470 + -0.0 3,054,471 - +1.4 3,054,576 + +0.9 3,054,576 + -1.3 3,054,576 + +0.4 3,054,576 + -0.4 3,054,775 + AO356_13480 0.50 -1.4 3,054,938 + AO356_13480 0.88 -0.7 3,054,938 + AO356_13480 0.88 -1.3 3,054,988 + -0.2 3,054,989 - +1.6 3,054,989 - +1.2 3,054,989 - -1.5 3,055,081 + AO356_13485 0.11 +0.3 3,055,082 - AO356_13485 0.12 -0.5 3,055,082 - AO356_13485 0.12 +0.0 3,055,082 - AO356_13485 0.12 +0.6 3,055,194 + AO356_13485 0.42 -0.2 3,055,339 + AO356_13485 0.82 -0.4 3,055,553 + AO356_13490 0.26 +0.3 3,055,553 + AO356_13490 0.26 -1.0 3,055,553 + AO356_13490 0.26 +1.0 3,055,553 + AO356_13490 0.26 +0.5 3,055,554 - AO356_13490 0.26 -0.7 3,055,554 - AO356_13490 0.26 +1.4 3,055,554 - AO356_13490 0.26 -0.3 3,055,554 - AO356_13490 0.26 -0.2 3,055,554 - AO356_13490 0.26 +0.2 3,055,554 - AO356_13490 0.26 +0.0
Or see this region's nucleotide sequence