Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_08865 and AO356_08870 are separated by 15 nucleotides AO356_08870 and AO356_08875 are separated by 107 nucleotides AO356_08875 and AO356_08880 overlap by 15 nucleotides
AO356_08865: AO356_08865 - lipid transporter ATP-binding/permease, at 1,998,171 to 1,999,976
_08865
AO356_08870: AO356_08870 - polymerase, at 1,999,992 to 2,001,164
_08870
AO356_08875: AO356_08875 - toluene tolerance protein, at 2,001,272 to 2,001,877
_08875
AO356_08880: AO356_08880 - glycosyl transferase, at 2,001,863 to 2,002,966
_08880
Position (kb)
1999
2000
2001
2002 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2000.079 kb on - strand at 2000.509 kb on + strand, within AO356_08870 at 2000.509 kb on + strand, within AO356_08870 at 2000.677 kb on + strand, within AO356_08870 at 2000.677 kb on + strand, within AO356_08870 at 2000.677 kb on + strand, within AO356_08870 at 2000.744 kb on + strand, within AO356_08870 at 2000.744 kb on + strand, within AO356_08870 at 2000.744 kb on + strand, within AO356_08870 at 2000.744 kb on + strand, within AO356_08870 at 2000.745 kb on - strand, within AO356_08870 at 2000.753 kb on - strand, within AO356_08870 at 2000.758 kb on + strand, within AO356_08870 at 2000.758 kb on + strand, within AO356_08870 at 2000.758 kb on + strand, within AO356_08870 at 2000.758 kb on + strand, within AO356_08870 at 2000.758 kb on + strand, within AO356_08870 at 2000.758 kb on + strand, within AO356_08870 at 2000.758 kb on + strand, within AO356_08870 at 2000.759 kb on - strand, within AO356_08870 at 2000.759 kb on - strand, within AO356_08870 at 2000.759 kb on - strand, within AO356_08870 at 2000.759 kb on - strand, within AO356_08870 at 2000.878 kb on + strand, within AO356_08870 at 2000.878 kb on + strand, within AO356_08870 at 2000.878 kb on + strand, within AO356_08870 at 2000.878 kb on + strand, within AO356_08870 at 2000.878 kb on + strand, within AO356_08870 at 2000.879 kb on - strand, within AO356_08870 at 2000.879 kb on - strand, within AO356_08870 at 2000.879 kb on - strand, within AO356_08870 at 2000.971 kb on + strand, within AO356_08870 at 2000.971 kb on + strand, within AO356_08870 at 2000.972 kb on - strand, within AO356_08870 at 2000.972 kb on - strand, within AO356_08870 at 2000.972 kb on - strand, within AO356_08870 at 2000.995 kb on - strand, within AO356_08870 at 2000.995 kb on - strand, within AO356_08870 at 2001.118 kb on - strand at 2001.166 kb on + strand at 2001.166 kb on + strand at 2001.166 kb on + strand at 2001.166 kb on + strand at 2001.166 kb on + strand at 2001.166 kb on + strand at 2001.167 kb on - strand at 2001.167 kb on - strand at 2001.175 kb on + strand at 2001.176 kb on - strand at 2001.185 kb on - strand at 2001.269 kb on - strand at 2001.549 kb on - strand, within AO356_08875 at 2001.614 kb on - strand, within AO356_08875 at 2001.807 kb on - strand, within AO356_08875 at 2001.809 kb on - strand, within AO356_08875 at 2001.809 kb on - strand, within AO356_08875 at 2002.027 kb on - strand, within AO356_08880
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 2,000,079 - -1.3 2,000,509 + AO356_08870 0.44 -1.3 2,000,509 + AO356_08870 0.44 -1.6 2,000,677 + AO356_08870 0.58 -2.6 2,000,677 + AO356_08870 0.58 -1.3 2,000,677 + AO356_08870 0.58 -2.0 2,000,744 + AO356_08870 0.64 -2.8 2,000,744 + AO356_08870 0.64 -0.0 2,000,744 + AO356_08870 0.64 -1.0 2,000,744 + AO356_08870 0.64 -0.7 2,000,745 - AO356_08870 0.64 -1.3 2,000,753 - AO356_08870 0.65 -1.8 2,000,758 + AO356_08870 0.65 -0.0 2,000,758 + AO356_08870 0.65 -2.3 2,000,758 + AO356_08870 0.65 -0.4 2,000,758 + AO356_08870 0.65 -1.0 2,000,758 + AO356_08870 0.65 -0.0 2,000,758 + AO356_08870 0.65 -0.0 2,000,758 + AO356_08870 0.65 -0.7 2,000,759 - AO356_08870 0.65 -1.0 2,000,759 - AO356_08870 0.65 -3.5 2,000,759 - AO356_08870 0.65 -1.0 2,000,759 - AO356_08870 0.65 -2.8 2,000,878 + AO356_08870 0.76 -0.0 2,000,878 + AO356_08870 0.76 -0.6 2,000,878 + AO356_08870 0.76 -1.0 2,000,878 + AO356_08870 0.76 -0.6 2,000,878 + AO356_08870 0.76 -0.7 2,000,879 - AO356_08870 0.76 -1.4 2,000,879 - AO356_08870 0.76 -1.7 2,000,879 - AO356_08870 0.76 -0.0 2,000,971 + AO356_08870 0.83 -1.0 2,000,971 + AO356_08870 0.83 +0.4 2,000,972 - AO356_08870 0.84 -1.0 2,000,972 - AO356_08870 0.84 -0.6 2,000,972 - AO356_08870 0.84 -1.3 2,000,995 - AO356_08870 0.86 +0.4 2,000,995 - AO356_08870 0.86 -1.3 2,001,118 - +1.6 2,001,166 + -0.6 2,001,166 + -0.0 2,001,166 + -0.0 2,001,166 + -1.6 2,001,166 + -0.0 2,001,166 + -1.0 2,001,167 - -0.0 2,001,167 - -0.0 2,001,175 + -1.4 2,001,176 - -0.8 2,001,185 - -1.0 2,001,269 - -0.0 2,001,549 - AO356_08875 0.46 +1.1 2,001,614 - AO356_08875 0.56 +0.6 2,001,807 - AO356_08875 0.88 -0.0 2,001,809 - AO356_08875 0.89 -0.0 2,001,809 - AO356_08875 0.89 -1.0 2,002,027 - AO356_08880 0.15 -1.0
Or see this region's nucleotide sequence