Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_08370 and AO356_08375 are separated by 9 nucleotides AO356_08375 and AO356_08380 are separated by 7 nucleotides AO356_08380 and AO356_08385 overlap by 4 nucleotides AO356_08385 and AO356_08390 are separated by 141 nucleotides
AO356_08370: AO356_08370 - urease subunit gamma, at 1,881,996 to 1,882,298
_08370
AO356_08375: AO356_08375 - acetyltransferase, at 1,882,308 to 1,882,841
_08375
AO356_08380: AO356_08380 - acetyltransferase, at 1,882,849 to 1,883,382
_08380
AO356_08385: AO356_08385 - urease subunit beta, at 1,883,379 to 1,883,684
_08385
AO356_08390: AO356_08390 - Urease (EC 3.5.1.5) <small>(from data)</small>, at 1,883,826 to 1,885,526
_08390
Position (kb) 1882
1883
1884 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1881.950 kb on + strand at 1881.950 kb on + strand at 1881.950 kb on + strand at 1881.950 kb on + strand at 1881.950 kb on + strand at 1881.950 kb on + strand at 1881.950 kb on + strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.951 kb on - strand at 1881.960 kb on + strand at 1881.974 kb on + strand at 1882.107 kb on + strand, within AO356_08370 at 1882.107 kb on + strand, within AO356_08370 at 1882.107 kb on + strand, within AO356_08370 at 1882.108 kb on - strand, within AO356_08370 at 1882.365 kb on + strand, within AO356_08375 at 1882.366 kb on - strand, within AO356_08375 at 1882.573 kb on - strand, within AO356_08375 at 1882.573 kb on - strand, within AO356_08375 at 1883.067 kb on - strand, within AO356_08380 at 1883.230 kb on + strand, within AO356_08380 at 1883.285 kb on - strand, within AO356_08380 at 1883.311 kb on + strand, within AO356_08380 at 1883.335 kb on + strand at 1883.335 kb on + strand at 1883.336 kb on - strand at 1883.336 kb on - strand at 1883.336 kb on - strand at 1883.336 kb on - strand at 1883.353 kb on + strand at 1883.353 kb on + strand at 1883.354 kb on - strand at 1883.354 kb on - strand at 1883.354 kb on - strand at 1883.394 kb on + strand at 1883.394 kb on + strand at 1883.496 kb on + strand, within AO356_08385 at 1883.497 kb on - strand, within AO356_08385 at 1883.497 kb on - strand, within AO356_08385 at 1883.682 kb on + strand at 1883.682 kb on + strand at 1883.682 kb on + strand at 1883.683 kb on - strand at 1883.683 kb on - strand at 1883.683 kb on - strand at 1883.689 kb on + strand at 1883.689 kb on + strand at 1883.689 kb on + strand at 1883.689 kb on + strand at 1883.690 kb on - strand at 1883.690 kb on - strand at 1883.690 kb on - strand at 1883.690 kb on - strand at 1883.737 kb on + strand at 1883.737 kb on + strand at 1883.937 kb on + strand at 1883.938 kb on - strand at 1883.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,881,950 + -1.0 1,881,950 + +0.5 1,881,950 + -0.0 1,881,950 + +0.1 1,881,950 + -0.3 1,881,950 + -0.2 1,881,950 + -0.5 1,881,951 - +0.7 1,881,951 - +1.1 1,881,951 - +0.5 1,881,951 - -0.8 1,881,951 - +0.4 1,881,951 - +0.4 1,881,951 - +1.5 1,881,951 - +0.3 1,881,951 - -0.2 1,881,960 + -0.8 1,881,974 + -0.7 1,882,107 + AO356_08370 0.37 +0.2 1,882,107 + AO356_08370 0.37 +0.3 1,882,107 + AO356_08370 0.37 -2.8 1,882,108 - AO356_08370 0.37 -0.6 1,882,365 + AO356_08375 0.11 +0.5 1,882,366 - AO356_08375 0.11 -0.0 1,882,573 - AO356_08375 0.50 -0.6 1,882,573 - AO356_08375 0.50 +0.3 1,883,067 - AO356_08380 0.41 -0.6 1,883,230 + AO356_08380 0.71 -0.5 1,883,285 - AO356_08380 0.82 +0.1 1,883,311 + AO356_08380 0.87 -1.5 1,883,335 + +0.4 1,883,335 + +0.3 1,883,336 - -0.0 1,883,336 - -0.0 1,883,336 - -0.7 1,883,336 - +0.3 1,883,353 + +1.0 1,883,353 + -0.4 1,883,354 - -0.0 1,883,354 - +0.2 1,883,354 - +0.5 1,883,394 + -0.0 1,883,394 + -0.0 1,883,496 + AO356_08385 0.38 -0.2 1,883,497 - AO356_08385 0.39 +0.6 1,883,497 - AO356_08385 0.39 +1.0 1,883,682 + -0.0 1,883,682 + -0.0 1,883,682 + +0.6 1,883,683 - +0.3 1,883,683 - -0.5 1,883,683 - +0.1 1,883,689 + +0.6 1,883,689 + +2.0 1,883,689 + -0.2 1,883,689 + -0.8 1,883,690 - +0.2 1,883,690 - +0.5 1,883,690 - +0.6 1,883,690 - -0.0 1,883,737 + -2.0 1,883,737 + -0.5 1,883,937 + -1.8 1,883,938 - +1.3 1,883,938 - -0.8
Or see this region's nucleotide sequence