Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_07380 and AO356_07385 are separated by 14 nucleotides AO356_07385 and AO356_07390 are separated by 125 nucleotides AO356_07390 and AO356_07395 are separated by 194 nucleotides AO356_07395 and AO356_07400 are separated by 143 nucleotides
AO356_07380: AO356_07380 - hypothetical protein, at 1,636,133 to 1,636,681
_07380
AO356_07385: AO356_07385 - tRNA-Thr, at 1,636,696 to 1,636,768
_07385
AO356_07390: AO356_07390 - polyribonucleotide nucleotidyltransferase, at 1,636,894 to 1,638,999
_07390
AO356_07395: AO356_07395 - 30S ribosomal protein S15, at 1,639,194 to 1,639,463
_07395
AO356_07400: AO356_07400 - pseudouridine synthase, at 1,639,607 to 1,640,524
_07400
Position (kb)
1636
1637
1638
1639 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1635.914 kb on - strand at 1635.981 kb on + strand at 1635.981 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.983 kb on + strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1635.984 kb on - strand at 1636.016 kb on + strand at 1636.016 kb on + strand at 1636.016 kb on + strand at 1636.016 kb on + strand at 1636.017 kb on - strand at 1636.017 kb on - strand at 1636.017 kb on - strand at 1636.017 kb on - strand at 1636.017 kb on - strand at 1636.017 kb on - strand at 1636.017 kb on - strand at 1636.017 kb on - strand at 1636.022 kb on + strand at 1636.022 kb on + strand at 1636.022 kb on + strand at 1636.023 kb on - strand at 1636.023 kb on - strand at 1636.023 kb on - strand at 1636.023 kb on - strand at 1636.023 kb on - strand at 1636.192 kb on + strand, within AO356_07380 at 1636.280 kb on + strand, within AO356_07380 at 1636.280 kb on + strand, within AO356_07380 at 1636.281 kb on - strand, within AO356_07380 at 1636.281 kb on - strand, within AO356_07380 at 1636.294 kb on + strand, within AO356_07380 at 1636.294 kb on + strand, within AO356_07380 at 1636.294 kb on + strand, within AO356_07380 at 1636.295 kb on - strand, within AO356_07380 at 1636.298 kb on + strand, within AO356_07380 at 1636.299 kb on - strand, within AO356_07380 at 1636.299 kb on - strand, within AO356_07380 at 1636.299 kb on - strand, within AO356_07380 at 1636.299 kb on - strand, within AO356_07380 at 1636.299 kb on - strand, within AO356_07380 at 1636.299 kb on - strand, within AO356_07380 at 1636.299 kb on - strand, within AO356_07380 at 1636.363 kb on + strand, within AO356_07380 at 1636.363 kb on + strand, within AO356_07380 at 1636.364 kb on - strand, within AO356_07380 at 1636.364 kb on - strand, within AO356_07380 at 1636.679 kb on + strand at 1636.680 kb on - strand at 1636.680 kb on - strand at 1636.680 kb on - strand at 1636.733 kb on - strand, within AO356_07385 at 1636.761 kb on - strand at 1636.761 kb on - strand at 1636.776 kb on - strand at 1636.781 kb on + strand at 1636.781 kb on + strand at 1636.781 kb on + strand at 1636.781 kb on + strand at 1636.782 kb on - strand at 1636.782 kb on - strand at 1637.123 kb on + strand, within AO356_07390 at 1637.124 kb on - strand, within AO356_07390 at 1639.756 kb on - strand, within AO356_07400 at 1639.938 kb on + strand, within AO356_07400 at 1639.939 kb on - strand, within AO356_07400 at 1639.990 kb on - strand, within AO356_07400 at 1639.990 kb on - strand, within AO356_07400 at 1639.990 kb on - strand, within AO356_07400 at 1639.990 kb on - strand, within AO356_07400 at 1639.990 kb on - strand, within AO356_07400
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,635,914 - -0.3 1,635,981 + -0.1 1,635,981 + -0.2 1,635,983 + +2.5 1,635,983 + +0.3 1,635,983 + -0.8 1,635,983 + +0.6 1,635,983 + -0.1 1,635,983 + +0.5 1,635,983 + +1.0 1,635,983 + +0.3 1,635,983 + +1.2 1,635,983 + -0.3 1,635,983 + -0.1 1,635,983 + -0.7 1,635,984 - -0.8 1,635,984 - -0.1 1,635,984 - +0.7 1,635,984 - +0.7 1,635,984 - +0.5 1,635,984 - +0.2 1,635,984 - +0.7 1,635,984 - -0.9 1,635,984 - +0.8 1,636,016 + -0.2 1,636,016 + +0.5 1,636,016 + -1.3 1,636,016 + +1.1 1,636,017 - +0.4 1,636,017 - -0.0 1,636,017 - +0.3 1,636,017 - -0.4 1,636,017 - -0.0 1,636,017 - -0.1 1,636,017 - +0.9 1,636,017 - +0.0 1,636,022 + +0.5 1,636,022 + +0.2 1,636,022 + -1.2 1,636,023 - +0.0 1,636,023 - -0.3 1,636,023 - -1.4 1,636,023 - -0.4 1,636,023 - -1.9 1,636,192 + AO356_07380 0.11 -0.7 1,636,280 + AO356_07380 0.27 -0.2 1,636,280 + AO356_07380 0.27 +0.8 1,636,281 - AO356_07380 0.27 +0.2 1,636,281 - AO356_07380 0.27 -0.2 1,636,294 + AO356_07380 0.29 -0.1 1,636,294 + AO356_07380 0.29 +0.4 1,636,294 + AO356_07380 0.29 -0.7 1,636,295 - AO356_07380 0.30 +0.5 1,636,298 + AO356_07380 0.30 +1.5 1,636,299 - AO356_07380 0.30 +0.4 1,636,299 - AO356_07380 0.30 -0.3 1,636,299 - AO356_07380 0.30 +0.8 1,636,299 - AO356_07380 0.30 -0.9 1,636,299 - AO356_07380 0.30 -0.4 1,636,299 - AO356_07380 0.30 -0.3 1,636,299 - AO356_07380 0.30 +0.8 1,636,363 + AO356_07380 0.42 -0.8 1,636,363 + AO356_07380 0.42 -0.1 1,636,364 - AO356_07380 0.42 +0.9 1,636,364 - AO356_07380 0.42 +0.3 1,636,679 + +0.3 1,636,680 - -0.7 1,636,680 - -0.4 1,636,680 - +0.9 1,636,733 - AO356_07385 0.51 +1.3 1,636,761 - -0.1 1,636,761 - -0.1 1,636,776 - -0.1 1,636,781 + +0.3 1,636,781 + -1.7 1,636,781 + -0.1 1,636,781 + -0.9 1,636,782 - -1.4 1,636,782 - -0.4 1,637,123 + AO356_07390 0.11 -1.7 1,637,124 - AO356_07390 0.11 -0.1 1,639,756 - AO356_07400 0.16 -1.4 1,639,938 + AO356_07400 0.36 -0.1 1,639,939 - AO356_07400 0.36 -1.1 1,639,990 - AO356_07400 0.42 -0.7 1,639,990 - AO356_07400 0.42 +0.6 1,639,990 - AO356_07400 0.42 -0.8 1,639,990 - AO356_07400 0.42 -2.1 1,639,990 - AO356_07400 0.42 -1.1
Or see this region's nucleotide sequence