Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04555 and AO356_04560 are separated by 61 nucleotides AO356_04560 and AO356_04565 are separated by 234 nucleotides
AO356_04555: AO356_04555 - peptidase M48, at 1,026,342 to 1,027,775
_04555
AO356_04560: AO356_04560 - serine peptidase, at 1,027,837 to 1,029,267
_04560
AO356_04565: AO356_04565 - RNA polymerase subunit sigma, at 1,029,502 to 1,030,449
_04565
Position (kb)
1027
1028
1029
1030 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1027.137 kb on + strand, within AO356_04555 at 1027.171 kb on - strand, within AO356_04555 at 1027.171 kb on - strand, within AO356_04555 at 1027.171 kb on - strand, within AO356_04555 at 1027.344 kb on + strand, within AO356_04555 at 1027.344 kb on + strand, within AO356_04555 at 1027.344 kb on + strand, within AO356_04555 at 1027.344 kb on + strand, within AO356_04555 at 1027.344 kb on + strand, within AO356_04555 at 1027.344 kb on + strand, within AO356_04555 at 1027.344 kb on + strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.345 kb on - strand, within AO356_04555 at 1027.429 kb on - strand, within AO356_04555 at 1027.440 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.554 kb on + strand, within AO356_04555 at 1027.555 kb on - strand, within AO356_04555 at 1027.620 kb on + strand, within AO356_04555 at 1027.620 kb on + strand, within AO356_04555 at 1027.621 kb on - strand, within AO356_04555 at 1027.790 kb on + strand at 1028.391 kb on + strand, within AO356_04560 at 1028.556 kb on - strand, within AO356_04560 at 1028.556 kb on - strand, within AO356_04560 at 1028.939 kb on + strand, within AO356_04560 at 1028.940 kb on - strand, within AO356_04560 at 1028.940 kb on - strand, within AO356_04560 at 1028.940 kb on - strand, within AO356_04560 at 1029.242 kb on + strand at 1029.243 kb on - strand at 1029.267 kb on + strand at 1029.267 kb on + strand at 1029.267 kb on + strand at 1029.268 kb on - strand at 1029.268 kb on - strand at 1029.294 kb on + strand at 1029.315 kb on + strand at 1029.328 kb on + strand at 1029.329 kb on - strand at 1029.329 kb on - strand at 1029.329 kb on - strand at 1029.336 kb on + strand at 1029.384 kb on + strand at 1029.387 kb on - strand at 1029.719 kb on + strand, within AO356_04565 at 1029.719 kb on + strand, within AO356_04565 at 1029.719 kb on + strand, within AO356_04565 at 1029.719 kb on + strand, within AO356_04565 at 1029.720 kb on - strand, within AO356_04565 at 1029.720 kb on - strand, within AO356_04565 at 1029.720 kb on - strand, within AO356_04565 at 1029.720 kb on - strand, within AO356_04565 at 1029.720 kb on - strand, within AO356_04565 at 1030.081 kb on + strand, within AO356_04565 at 1030.149 kb on + strand, within AO356_04565 at 1030.191 kb on + strand, within AO356_04565 at 1030.191 kb on + strand, within AO356_04565 at 1030.191 kb on + strand, within AO356_04565 at 1030.191 kb on + strand, within AO356_04565 at 1030.191 kb on + strand, within AO356_04565 at 1030.192 kb on - strand, within AO356_04565 at 1030.192 kb on - strand, within AO356_04565
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,027,137 + AO356_04555 0.55 +0.3 1,027,171 - AO356_04555 0.58 -0.4 1,027,171 - AO356_04555 0.58 -1.4 1,027,171 - AO356_04555 0.58 -0.3 1,027,344 + AO356_04555 0.70 -1.3 1,027,344 + AO356_04555 0.70 -0.1 1,027,344 + AO356_04555 0.70 -0.3 1,027,344 + AO356_04555 0.70 -2.7 1,027,344 + AO356_04555 0.70 -0.1 1,027,344 + AO356_04555 0.70 -1.9 1,027,344 + AO356_04555 0.70 -0.6 1,027,345 - AO356_04555 0.70 -0.7 1,027,345 - AO356_04555 0.70 -1.2 1,027,345 - AO356_04555 0.70 -0.4 1,027,345 - AO356_04555 0.70 -0.3 1,027,345 - AO356_04555 0.70 -0.5 1,027,345 - AO356_04555 0.70 -0.7 1,027,345 - AO356_04555 0.70 -0.7 1,027,345 - AO356_04555 0.70 -1.2 1,027,429 - AO356_04555 0.76 +0.4 1,027,440 + AO356_04555 0.77 -2.5 1,027,554 + AO356_04555 0.85 -0.3 1,027,554 + AO356_04555 0.85 +0.1 1,027,554 + AO356_04555 0.85 -1.5 1,027,554 + AO356_04555 0.85 -0.5 1,027,554 + AO356_04555 0.85 -0.0 1,027,554 + AO356_04555 0.85 -0.9 1,027,554 + AO356_04555 0.85 -1.5 1,027,554 + AO356_04555 0.85 -0.0 1,027,555 - AO356_04555 0.85 -1.0 1,027,620 + AO356_04555 0.89 -1.0 1,027,620 + AO356_04555 0.89 -0.4 1,027,621 - AO356_04555 0.89 -1.0 1,027,790 + -0.5 1,028,391 + AO356_04560 0.39 -0.3 1,028,556 - AO356_04560 0.50 -2.6 1,028,556 - AO356_04560 0.50 -0.3 1,028,939 + AO356_04560 0.77 -0.3 1,028,940 - AO356_04560 0.77 -0.3 1,028,940 - AO356_04560 0.77 -1.3 1,028,940 - AO356_04560 0.77 -0.3 1,029,242 + +0.7 1,029,243 - -0.3 1,029,267 + -2.3 1,029,267 + -1.6 1,029,267 + -0.3 1,029,268 - -1.9 1,029,268 - -1.3 1,029,294 + -1.9 1,029,315 + -1.3 1,029,328 + -0.3 1,029,329 - +0.9 1,029,329 - +2.5 1,029,329 - +0.1 1,029,336 + -1.9 1,029,384 + -3.3 1,029,387 - +0.0 1,029,719 + AO356_04565 0.23 +0.9 1,029,719 + AO356_04565 0.23 +0.5 1,029,719 + AO356_04565 0.23 +0.5 1,029,719 + AO356_04565 0.23 +0.4 1,029,720 - AO356_04565 0.23 -0.4 1,029,720 - AO356_04565 0.23 -0.3 1,029,720 - AO356_04565 0.23 -0.5 1,029,720 - AO356_04565 0.23 +0.4 1,029,720 - AO356_04565 0.23 -0.5 1,030,081 + AO356_04565 0.61 +0.4 1,030,149 + AO356_04565 0.68 -0.6 1,030,191 + AO356_04565 0.73 +0.2 1,030,191 + AO356_04565 0.73 -0.3 1,030,191 + AO356_04565 0.73 +0.4 1,030,191 + AO356_04565 0.73 +0.2 1,030,191 + AO356_04565 0.73 -0.1 1,030,192 - AO356_04565 0.73 +0.7 1,030,192 - AO356_04565 0.73 -0.1
Or see this region's nucleotide sequence