Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04050 and AO356_04055 are separated by 422 nucleotides AO356_04055 and AO356_04060 are separated by 8 nucleotides AO356_04060 and AO356_04065 are separated by 348 nucleotides
AO356_04050: AO356_04050 - ATP-dependent DNA helicase RecQ, at 901,191 to 903,317
_04050
AO356_04055: AO356_04055 - hypothetical protein, at 903,740 to 904,327
_04055
AO356_04060: AO356_04060 - hypothetical protein, at 904,336 to 904,731
_04060
AO356_04065: AO356_04065 - type I secretion target, at 905,080 to 913,647
_04065
Position (kb) 903
904
905 Strain fitness (log2 ratio) -2
-1
0
1
2 at 902.962 kb on - strand, within AO356_04050 at 902.983 kb on + strand, within AO356_04050 at 903.243 kb on - strand at 903.317 kb on + strand at 903.317 kb on + strand at 903.317 kb on + strand at 903.318 kb on - strand at 903.318 kb on - strand at 903.390 kb on + strand at 903.391 kb on - strand at 903.391 kb on - strand at 903.444 kb on + strand at 903.444 kb on + strand at 903.444 kb on + strand at 903.444 kb on + strand at 903.444 kb on + strand at 903.445 kb on - strand at 903.619 kb on + strand at 903.619 kb on + strand at 903.619 kb on + strand at 903.619 kb on + strand at 903.619 kb on + strand at 903.665 kb on + strand at 903.666 kb on - strand at 903.685 kb on + strand at 903.686 kb on - strand at 903.701 kb on + strand at 903.702 kb on - strand at 903.720 kb on - strand at 903.739 kb on - strand at 903.816 kb on - strand, within AO356_04055 at 903.816 kb on - strand, within AO356_04055 at 903.914 kb on + strand, within AO356_04055 at 904.383 kb on - strand, within AO356_04060 at 904.504 kb on + strand, within AO356_04060 at 904.552 kb on + strand, within AO356_04060 at 904.553 kb on - strand, within AO356_04060 at 904.592 kb on - strand, within AO356_04060 at 904.592 kb on - strand, within AO356_04060 at 904.592 kb on - strand, within AO356_04060 at 904.696 kb on + strand at 904.696 kb on + strand at 904.696 kb on + strand at 904.696 kb on + strand at 904.696 kb on + strand at 904.696 kb on + strand at 904.697 kb on - strand at 904.697 kb on - strand at 904.697 kb on - strand at 904.697 kb on - strand at 904.697 kb on - strand at 904.698 kb on + strand at 904.699 kb on - strand at 904.699 kb on - strand at 904.764 kb on + strand at 904.765 kb on - strand at 904.765 kb on - strand at 904.765 kb on - strand at 904.988 kb on + strand at 904.988 kb on + strand at 904.988 kb on + strand at 904.989 kb on - strand at 904.989 kb on - strand at 905.024 kb on + strand at 905.024 kb on + strand at 905.024 kb on + strand at 905.025 kb on - strand at 905.025 kb on - strand at 905.025 kb on - strand at 905.088 kb on + strand at 905.306 kb on + strand at 905.306 kb on + strand at 905.306 kb on + strand at 905.306 kb on + strand at 905.306 kb on + strand at 905.307 kb on - strand at 905.307 kb on - strand at 905.307 kb on - strand at 905.307 kb on - strand at 905.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 902,962 - AO356_04050 0.83 -0.2 902,983 + AO356_04050 0.84 -0.3 903,243 - -0.5 903,317 + -0.2 903,317 + -1.9 903,317 + -1.0 903,318 - +2.0 903,318 - -0.1 903,390 + +0.0 903,391 - -0.2 903,391 - -0.2 903,444 + +1.6 903,444 + +0.1 903,444 + +0.1 903,444 + -0.4 903,444 + +0.2 903,445 - -0.1 903,619 + +0.3 903,619 + +0.3 903,619 + +0.3 903,619 + +0.3 903,619 + -0.5 903,665 + -0.2 903,666 - +1.0 903,685 + -0.3 903,686 - -1.5 903,701 + +0.4 903,702 - +0.0 903,720 - -0.6 903,739 - -2.6 903,816 - AO356_04055 0.13 -0.3 903,816 - AO356_04055 0.13 -1.9 903,914 + AO356_04055 0.30 +0.3 904,383 - AO356_04060 0.12 +0.5 904,504 + AO356_04060 0.42 -0.1 904,552 + AO356_04060 0.55 +0.2 904,553 - AO356_04060 0.55 -0.2 904,592 - AO356_04060 0.65 -0.8 904,592 - AO356_04060 0.65 +0.4 904,592 - AO356_04060 0.65 +0.1 904,696 + -0.1 904,696 + +0.0 904,696 + +1.3 904,696 + +0.2 904,696 + +0.4 904,696 + -0.3 904,697 - +0.9 904,697 - +0.1 904,697 - -0.9 904,697 - +0.7 904,697 - -1.0 904,698 + +0.6 904,699 - -2.1 904,699 - -0.3 904,764 + -0.3 904,765 - +1.3 904,765 - +1.6 904,765 - -1.1 904,988 + -0.7 904,988 + -0.3 904,988 + +0.3 904,989 - +0.7 904,989 - -0.1 905,024 + -0.4 905,024 + -0.9 905,024 + -0.8 905,025 - +0.3 905,025 - -0.6 905,025 - -0.1 905,088 + +0.3 905,306 + +0.9 905,306 + -0.2 905,306 + -1.0 905,306 + -1.5 905,306 + +0.5 905,307 - +0.6 905,307 - +0.1 905,307 - -0.6 905,307 - +0.4 905,307 - -0.6
Or see this region's nucleotide sequence