Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_03610 and AO356_03615 are separated by 387 nucleotides AO356_03615 and AO356_03620 are separated by 14 nucleotides AO356_03620 and AO356_03625 overlap by 8 nucleotides AO356_03625 and AO356_03630 overlap by 4 nucleotides AO356_03630 and AO356_03635 overlap by 4 nucleotides
AO356_03610: AO356_03610 - hypothetical protein, at 806,970 to 809,063
_03610
AO356_03615: AO356_03615 - hypothetical protein, at 809,451 to 809,858
_03615
AO356_03620: AO356_03620 - cytochrome C, at 809,873 to 811,075
_03620
AO356_03625: AO356_03625 - cytochrome C, at 811,068 to 811,541
_03625
AO356_03630: AO356_03630 - thiol:disulfide interchange protein, at 811,538 to 812,074
_03630
AO356_03635: AO356_03635 - cytochrome C biogenesis protein CcmF, at 812,071 to 814,059
_03635
Position (kb)
809
810
811
812 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 808.918 kb on + strand at 808.918 kb on + strand at 808.918 kb on + strand at 808.918 kb on + strand at 808.918 kb on + strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.947 kb on + strand at 808.948 kb on - strand at 808.948 kb on - strand at 808.952 kb on + strand at 808.952 kb on + strand at 808.952 kb on + strand at 808.952 kb on + strand at 808.952 kb on + strand at 808.952 kb on + strand at 808.953 kb on - strand at 808.953 kb on - strand at 808.953 kb on - strand at 808.953 kb on - strand at 808.984 kb on + strand at 808.985 kb on - strand at 809.071 kb on - strand at 809.181 kb on - strand at 809.194 kb on + strand at 809.194 kb on + strand at 809.194 kb on + strand at 809.194 kb on + strand at 809.195 kb on - strand at 809.314 kb on + strand at 809.314 kb on + strand at 809.314 kb on + strand at 809.314 kb on + strand at 809.315 kb on - strand at 809.332 kb on + strand at 809.333 kb on - strand at 809.555 kb on - strand, within AO356_03615 at 809.557 kb on + strand, within AO356_03615 at 809.557 kb on + strand, within AO356_03615 at 809.557 kb on + strand, within AO356_03615 at 809.557 kb on + strand, within AO356_03615 at 809.557 kb on + strand, within AO356_03615 at 809.557 kb on + strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.558 kb on - strand, within AO356_03615 at 809.561 kb on + strand, within AO356_03615 at 809.561 kb on + strand, within AO356_03615 at 809.561 kb on + strand, within AO356_03615 at 809.562 kb on - strand, within AO356_03615 at 809.572 kb on + strand, within AO356_03615 at 809.573 kb on - strand, within AO356_03615 at 809.579 kb on + strand, within AO356_03615 at 809.579 kb on + strand, within AO356_03615 at 809.579 kb on + strand, within AO356_03615 at 809.579 kb on + strand, within AO356_03615 at 809.579 kb on + strand, within AO356_03615 at 809.579 kb on + strand, within AO356_03615 at 809.579 kb on + strand, within AO356_03615 at 809.580 kb on - strand, within AO356_03615 at 809.580 kb on - strand, within AO356_03615 at 809.580 kb on - strand, within AO356_03615 at 809.580 kb on - strand, within AO356_03615 at 809.580 kb on - strand, within AO356_03615 at 809.580 kb on - strand, within AO356_03615 at 809.602 kb on + strand, within AO356_03615 at 809.602 kb on + strand, within AO356_03615 at 809.603 kb on - strand, within AO356_03615 at 809.603 kb on - strand, within AO356_03615 at 809.603 kb on - strand, within AO356_03615 at 809.603 kb on - strand, within AO356_03615 at 809.841 kb on - strand at 809.874 kb on + strand at 810.367 kb on - strand, within AO356_03620 at 810.492 kb on + strand, within AO356_03620 at 810.493 kb on - strand, within AO356_03620 at 810.615 kb on + strand, within AO356_03620 at 810.616 kb on - strand, within AO356_03620 at 810.631 kb on + strand, within AO356_03620 at 810.948 kb on + strand, within AO356_03620 at 811.086 kb on - strand at 811.145 kb on + strand, within AO356_03625 at 811.146 kb on - strand, within AO356_03625 at 811.146 kb on - strand, within AO356_03625 at 811.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 808,918 + -1.3 808,918 + +1.1 808,918 + -0.1 808,918 + -0.4 808,918 + +0.6 808,919 - -0.0 808,919 - +0.2 808,919 - -1.4 808,919 - -0.1 808,919 - +0.3 808,919 - -0.0 808,919 - +0.1 808,919 - +1.8 808,919 - +0.6 808,947 + +0.3 808,948 - +0.3 808,948 - +0.6 808,952 + +0.6 808,952 + +0.3 808,952 + +0.1 808,952 + +0.3 808,952 + +0.7 808,952 + -0.2 808,953 - +0.2 808,953 - -0.4 808,953 - -0.7 808,953 - +1.4 808,984 + -0.1 808,985 - -0.2 809,071 - -0.2 809,181 - -1.4 809,194 + -0.0 809,194 + -0.6 809,194 + +1.1 809,194 + +1.2 809,195 - +0.6 809,314 + +0.2 809,314 + -0.1 809,314 + -0.8 809,314 + +0.4 809,315 - +0.6 809,332 + +0.1 809,333 - -0.0 809,555 - AO356_03615 0.25 -0.3 809,557 + AO356_03615 0.26 +0.2 809,557 + AO356_03615 0.26 -0.4 809,557 + AO356_03615 0.26 -1.9 809,557 + AO356_03615 0.26 +0.6 809,557 + AO356_03615 0.26 +0.4 809,557 + AO356_03615 0.26 -0.4 809,558 - AO356_03615 0.26 -0.1 809,558 - AO356_03615 0.26 +1.5 809,558 - AO356_03615 0.26 +0.0 809,558 - AO356_03615 0.26 +0.1 809,558 - AO356_03615 0.26 -0.8 809,558 - AO356_03615 0.26 -0.8 809,558 - AO356_03615 0.26 +0.3 809,558 - AO356_03615 0.26 -0.1 809,561 + AO356_03615 0.27 -0.2 809,561 + AO356_03615 0.27 +0.8 809,561 + AO356_03615 0.27 +1.1 809,562 - AO356_03615 0.27 -0.3 809,572 + AO356_03615 0.30 +0.3 809,573 - AO356_03615 0.30 -0.0 809,579 + AO356_03615 0.31 -0.5 809,579 + AO356_03615 0.31 +0.4 809,579 + AO356_03615 0.31 +0.2 809,579 + AO356_03615 0.31 +0.4 809,579 + AO356_03615 0.31 -0.2 809,579 + AO356_03615 0.31 -0.4 809,579 + AO356_03615 0.31 +0.2 809,580 - AO356_03615 0.32 +0.3 809,580 - AO356_03615 0.32 +0.4 809,580 - AO356_03615 0.32 +0.5 809,580 - AO356_03615 0.32 +0.8 809,580 - AO356_03615 0.32 -0.4 809,580 - AO356_03615 0.32 -0.5 809,602 + AO356_03615 0.37 +0.1 809,602 + AO356_03615 0.37 +0.6 809,603 - AO356_03615 0.37 -1.6 809,603 - AO356_03615 0.37 -0.3 809,603 - AO356_03615 0.37 +0.2 809,603 - AO356_03615 0.37 -0.6 809,841 - -0.8 809,874 + +1.5 810,367 - AO356_03620 0.41 -0.2 810,492 + AO356_03620 0.51 +0.1 810,493 - AO356_03620 0.52 -0.8 810,615 + AO356_03620 0.62 -1.5 810,616 - AO356_03620 0.62 -0.8 810,631 + AO356_03620 0.63 -0.4 810,948 + AO356_03620 0.89 -1.9 811,086 - -0.0 811,145 + AO356_03625 0.16 -1.4 811,146 - AO356_03625 0.16 -0.4 811,146 - AO356_03625 0.16 -0.9 811,570 - -0.4
Or see this region's nucleotide sequence