Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_01240 and AO356_01245 are separated by 157 nucleotides AO356_01245 and AO356_01250 are separated by 11 nucleotides
AO356_01240: AO356_01240 - glucarate dehydratase, at 284,321 to 285,664
_01240
AO356_01245: AO356_01245 - glycogen branching protein, at 285,822 to 288,056
_01245
AO356_01250: AO356_01250 - alpha-amylase, at 288,068 to 291,412
_01250
Position (kb) 285
286
287
288
289 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 285.563 kb on + strand at 285.563 kb on + strand at 285.564 kb on - strand at 285.564 kb on - strand at 285.823 kb on + strand at 285.823 kb on + strand at 285.824 kb on - strand at 285.824 kb on - strand at 285.824 kb on - strand at 285.934 kb on + strand at 285.950 kb on - strand at 285.950 kb on - strand at 286.061 kb on + strand, within AO356_01245 at 286.091 kb on - strand, within AO356_01245 at 286.342 kb on + strand, within AO356_01245 at 286.462 kb on + strand, within AO356_01245 at 286.462 kb on + strand, within AO356_01245 at 286.463 kb on - strand, within AO356_01245 at 286.463 kb on - strand, within AO356_01245 at 286.463 kb on - strand, within AO356_01245 at 286.463 kb on - strand, within AO356_01245 at 286.525 kb on + strand, within AO356_01245 at 286.525 kb on + strand, within AO356_01245 at 286.564 kb on + strand, within AO356_01245 at 286.876 kb on + strand, within AO356_01245 at 286.877 kb on - strand, within AO356_01245 at 286.877 kb on - strand, within AO356_01245 at 287.176 kb on + strand, within AO356_01245 at 287.177 kb on - strand, within AO356_01245 at 287.177 kb on - strand, within AO356_01245 at 287.212 kb on + strand, within AO356_01245 at 287.213 kb on - strand, within AO356_01245 at 287.275 kb on + strand, within AO356_01245 at 287.275 kb on + strand, within AO356_01245 at 287.276 kb on - strand, within AO356_01245 at 287.456 kb on - strand, within AO356_01245 at 287.461 kb on + strand, within AO356_01245 at 287.693 kb on - strand, within AO356_01245 at 287.693 kb on - strand, within AO356_01245 at 287.728 kb on + strand, within AO356_01245 at 287.728 kb on + strand, within AO356_01245 at 287.780 kb on - strand, within AO356_01245 at 287.780 kb on - strand, within AO356_01245 at 287.780 kb on - strand, within AO356_01245 at 287.780 kb on - strand, within AO356_01245 at 288.069 kb on + strand at 288.171 kb on + strand at 288.184 kb on - strand at 288.184 kb on - strand at 288.260 kb on - strand at 288.277 kb on - strand at 288.277 kb on - strand at 288.312 kb on + strand at 288.313 kb on - strand at 288.313 kb on - strand at 288.396 kb on + strand at 288.433 kb on - strand, within AO356_01250 at 288.433 kb on - strand, within AO356_01250 at 288.498 kb on + strand, within AO356_01250 at 288.499 kb on - strand, within AO356_01250 at 288.499 kb on - strand, within AO356_01250 at 288.795 kb on - strand, within AO356_01250 at 288.864 kb on + strand, within AO356_01250 at 288.864 kb on + strand, within AO356_01250 at 288.864 kb on + strand, within AO356_01250 at 288.864 kb on + strand, within AO356_01250 at 288.865 kb on - strand, within AO356_01250 at 288.966 kb on + strand, within AO356_01250 at 288.967 kb on - strand, within AO356_01250 at 289.043 kb on - strand, within AO356_01250
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 285,563 + +0.4 285,563 + +0.8 285,564 - +0.9 285,564 - +0.6 285,823 + +0.3 285,823 + +0.8 285,824 - +1.1 285,824 - -0.2 285,824 - +0.4 285,934 + -2.0 285,950 - -3.0 285,950 - -1.5 286,061 + AO356_01245 0.11 +0.5 286,091 - AO356_01245 0.12 -1.8 286,342 + AO356_01245 0.23 -2.0 286,462 + AO356_01245 0.29 -1.2 286,462 + AO356_01245 0.29 -2.0 286,463 - AO356_01245 0.29 +1.5 286,463 - AO356_01245 0.29 -2.6 286,463 - AO356_01245 0.29 -0.5 286,463 - AO356_01245 0.29 -0.5 286,525 + AO356_01245 0.31 -0.9 286,525 + AO356_01245 0.31 -2.0 286,564 + AO356_01245 0.33 -2.0 286,876 + AO356_01245 0.47 -0.8 286,877 - AO356_01245 0.47 -0.1 286,877 - AO356_01245 0.47 +1.5 287,176 + AO356_01245 0.61 -0.5 287,177 - AO356_01245 0.61 -0.5 287,177 - AO356_01245 0.61 -1.5 287,212 + AO356_01245 0.62 +0.5 287,213 - AO356_01245 0.62 -1.5 287,275 + AO356_01245 0.65 -1.2 287,275 + AO356_01245 0.65 +0.5 287,276 - AO356_01245 0.65 -2.8 287,456 - AO356_01245 0.73 -0.5 287,461 + AO356_01245 0.73 -1.5 287,693 - AO356_01245 0.84 -1.2 287,693 - AO356_01245 0.84 -1.5 287,728 + AO356_01245 0.85 -1.5 287,728 + AO356_01245 0.85 -1.7 287,780 - AO356_01245 0.88 -1.8 287,780 - AO356_01245 0.88 +0.1 287,780 - AO356_01245 0.88 -0.5 287,780 - AO356_01245 0.88 -2.5 288,069 + -1.0 288,171 + -0.5 288,184 - +0.1 288,184 - -0.1 288,260 - +0.2 288,277 - +0.7 288,277 - +2.0 288,312 + -1.8 288,313 - -0.7 288,313 - -2.0 288,396 + -0.8 288,433 - AO356_01250 0.11 +1.7 288,433 - AO356_01250 0.11 +0.3 288,498 + AO356_01250 0.13 +0.6 288,499 - AO356_01250 0.13 +0.1 288,499 - AO356_01250 0.13 +0.1 288,795 - AO356_01250 0.22 -1.5 288,864 + AO356_01250 0.24 +0.5 288,864 + AO356_01250 0.24 -0.5 288,864 + AO356_01250 0.24 +0.0 288,864 + AO356_01250 0.24 +0.3 288,865 - AO356_01250 0.24 +0.1 288,966 + AO356_01250 0.27 +0.8 288,967 - AO356_01250 0.27 -0.0 289,043 - AO356_01250 0.29 -2.0
Or see this region's nucleotide sequence