Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05695 and HSERO_RS05700 are separated by 6 nucleotides HSERO_RS05700 and HSERO_RS05705 are separated by 127 nucleotides HSERO_RS05705 and HSERO_RS05710 are separated by 55 nucleotides
HSERO_RS05695: HSERO_RS05695 - D-amino acid oxidase, at 1,293,990 to 1,295,315
_RS05695
HSERO_RS05700: HSERO_RS05700 - LysR family transcriptional regulator, at 1,295,322 to 1,296,266
_RS05700
HSERO_RS05705: HSERO_RS05705 - endoribonuclease L-PSP, at 1,296,394 to 1,296,810
_RS05705
HSERO_RS05710: HSERO_RS05710 - aldehyde dehydrogenase, at 1,296,866 to 1,298,326
_RS05710
Position (kb)
1295
1296
1297 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1294.654 kb on + strand, within HSERO_RS05695 at 1294.750 kb on + strand, within HSERO_RS05695 at 1294.919 kb on - strand, within HSERO_RS05695 at 1295.071 kb on - strand, within HSERO_RS05695 at 1295.143 kb on + strand, within HSERO_RS05695 at 1295.143 kb on + strand, within HSERO_RS05695 at 1295.151 kb on - strand, within HSERO_RS05695 at 1295.151 kb on - strand, within HSERO_RS05695 at 1295.151 kb on - strand, within HSERO_RS05695 at 1295.233 kb on - strand at 1295.328 kb on - strand at 1295.395 kb on - strand at 1295.422 kb on - strand, within HSERO_RS05700 at 1295.678 kb on + strand, within HSERO_RS05700 at 1295.680 kb on - strand, within HSERO_RS05700 at 1295.797 kb on + strand, within HSERO_RS05700 at 1296.010 kb on - strand, within HSERO_RS05700 at 1296.042 kb on - strand, within HSERO_RS05700 at 1296.283 kb on - strand at 1296.296 kb on + strand at 1296.370 kb on - strand at 1296.370 kb on - strand at 1296.443 kb on + strand, within HSERO_RS05705 at 1296.457 kb on + strand, within HSERO_RS05705 at 1296.659 kb on + strand at 1296.710 kb on - strand, within HSERO_RS05705 at 1296.799 kb on + strand at 1296.835 kb on - strand at 1296.916 kb on + strand at 1296.952 kb on - strand at 1296.960 kb on + strand at 1296.981 kb on + strand at 1297.020 kb on - strand, within HSERO_RS05710 at 1297.020 kb on - strand, within HSERO_RS05710 at 1297.189 kb on - strand, within HSERO_RS05710 at 1297.189 kb on - strand, within HSERO_RS05710
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 1,294,654 + HSERO_RS05695 0.50 -1.1 1,294,750 + HSERO_RS05695 0.57 -1.4 1,294,919 - HSERO_RS05695 0.70 -1.5 1,295,071 - HSERO_RS05695 0.82 +0.1 1,295,143 + HSERO_RS05695 0.87 +3.6 1,295,143 + HSERO_RS05695 0.87 +0.7 1,295,151 - HSERO_RS05695 0.88 -0.0 1,295,151 - HSERO_RS05695 0.88 -0.5 1,295,151 - HSERO_RS05695 0.88 +1.7 1,295,233 - +1.0 1,295,328 - -1.6 1,295,395 - +0.2 1,295,422 - HSERO_RS05700 0.11 +0.3 1,295,678 + HSERO_RS05700 0.38 +0.2 1,295,680 - HSERO_RS05700 0.38 +0.2 1,295,797 + HSERO_RS05700 0.50 +1.3 1,296,010 - HSERO_RS05700 0.73 -0.2 1,296,042 - HSERO_RS05700 0.76 +0.2 1,296,283 - +0.0 1,296,296 + -0.8 1,296,370 - -0.3 1,296,370 - -1.2 1,296,443 + HSERO_RS05705 0.12 +0.3 1,296,457 + HSERO_RS05705 0.15 +1.1 1,296,659 + +1.0 1,296,710 - HSERO_RS05705 0.76 +0.7 1,296,799 + +0.4 1,296,835 - +0.1 1,296,916 + +0.3 1,296,952 - +0.1 1,296,960 + +1.4 1,296,981 + +0.1 1,297,020 - HSERO_RS05710 0.11 +0.3 1,297,020 - HSERO_RS05710 0.11 -0.6 1,297,189 - HSERO_RS05710 0.22 -1.2 1,297,189 - HSERO_RS05710 0.22 -0.1
Or see this region's nucleotide sequence