Experiment: m.b. 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09530 and PGA1_c09540 are separated by 27 nucleotides PGA1_c09540 and PGA1_c09550 are separated by 331 nucleotides
PGA1_c09530: PGA1_c09530 - UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase MurF, at 977,137 to 978,549
_c09530
PGA1_c09540: PGA1_c09540 - phospho-N-acetylmuramoyl-pentapeptide- transferase MraY, at 978,577 to 979,659
_c09540
PGA1_c09550: PGA1_c09550 - Glutaminase (EC 3.5.1.2) (from data) , at 979,991 to 980,905
_c09550
Position (kb)
978
979
980 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 979.653 kb on + strand at 979.840 kb on + strand at 979.840 kb on + strand at 979.924 kb on + strand at 979.924 kb on + strand at 979.932 kb on - strand at 979.937 kb on + strand at 979.938 kb on - strand at 979.938 kb on - strand at 980.066 kb on + strand at 980.074 kb on - strand at 980.113 kb on + strand, within PGA1_c09550 at 980.206 kb on - strand, within PGA1_c09550 at 980.238 kb on - strand, within PGA1_c09550 at 980.291 kb on - strand, within PGA1_c09550 at 980.358 kb on + strand, within PGA1_c09550 at 980.358 kb on + strand, within PGA1_c09550 at 980.400 kb on + strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.487 kb on - strand, within PGA1_c09550 at 980.515 kb on + strand, within PGA1_c09550 at 980.515 kb on + strand, within PGA1_c09550 at 980.520 kb on + strand, within PGA1_c09550 at 980.536 kb on + strand, within PGA1_c09550 at 980.544 kb on + strand, within PGA1_c09550 at 980.556 kb on - strand, within PGA1_c09550 at 980.623 kb on - strand, within PGA1_c09550 at 980.635 kb on - strand, within PGA1_c09550
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 5-Hydroxymethylfurfural 2.5 mM remove 979,653 + +0.2 979,840 + -1.3 979,840 + -1.0 979,924 + -0.1 979,924 + +0.4 979,932 - -0.5 979,937 + +0.1 979,938 - +0.3 979,938 - -0.2 980,066 + +2.0 980,074 - -0.1 980,113 + PGA1_c09550 0.13 -2.0 980,206 - PGA1_c09550 0.23 -0.6 980,238 - PGA1_c09550 0.27 -0.2 980,291 - PGA1_c09550 0.33 +0.6 980,358 + PGA1_c09550 0.40 +0.7 980,358 + PGA1_c09550 0.40 -0.5 980,400 + PGA1_c09550 0.45 -1.6 980,408 - PGA1_c09550 0.46 -2.0 980,408 - PGA1_c09550 0.46 -0.7 980,408 - PGA1_c09550 0.46 -0.7 980,408 - PGA1_c09550 0.46 +0.3 980,487 - PGA1_c09550 0.54 -0.0 980,515 + PGA1_c09550 0.57 +0.5 980,515 + PGA1_c09550 0.57 -0.2 980,520 + PGA1_c09550 0.58 +0.7 980,536 + PGA1_c09550 0.60 -2.0 980,544 + PGA1_c09550 0.60 -1.3 980,556 - PGA1_c09550 0.62 -0.1 980,623 - PGA1_c09550 0.69 -0.2 980,635 - PGA1_c09550 0.70 +0.6
Or see this region's nucleotide sequence