Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_29495 and AO356_29500 are separated by 32 nucleotides AO356_29500 and AO356_29505 are separated by 191 nucleotides AO356_29505 and AO356_29510 are separated by 1 nucleotides AO356_29510 and AO356_29515 are separated by 52 nucleotides
AO356_29495: AO356_29495 - NADPH:quinone oxidoreductase, at 6,860,598 to 6,861,572
_29495
AO356_29500: AO356_29500 - monooxygenase, at 6,861,605 to 6,861,964
_29500
AO356_29505: AO356_29505 - biopolymer transporter ExbD, at 6,862,156 to 6,862,557
_29505
AO356_29510: AO356_29510 - biopolymer transporter ExbB, at 6,862,559 to 6,863,278
_29510
AO356_29515: AO356_29515 - energy transducer TonB, at 6,863,331 to 6,864,140
_29515
Position (kb)
6862
6863
6864 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6861.584 kb on + strand at 6861.584 kb on + strand at 6861.612 kb on - strand at 6861.765 kb on + strand, within AO356_29500 at 6861.766 kb on - strand, within AO356_29500 at 6861.766 kb on - strand, within AO356_29500 at 6861.910 kb on + strand, within AO356_29500 at 6861.910 kb on + strand, within AO356_29500 at 6861.938 kb on - strand at 6861.938 kb on - strand at 6861.994 kb on + strand at 6861.994 kb on + strand at 6861.994 kb on + strand at 6861.994 kb on + strand at 6861.995 kb on - strand at 6862.026 kb on - strand at 6862.026 kb on - strand at 6862.042 kb on + strand at 6862.082 kb on + strand at 6862.082 kb on + strand at 6862.089 kb on + strand at 6862.089 kb on + strand at 6862.089 kb on + strand at 6862.089 kb on + strand at 6862.090 kb on - strand at 6862.090 kb on - strand at 6862.090 kb on - strand at 6862.090 kb on - strand at 6862.090 kb on - strand at 6862.090 kb on - strand at 6862.157 kb on + strand at 6862.158 kb on - strand at 6862.158 kb on - strand at 6862.232 kb on + strand, within AO356_29505 at 6862.233 kb on - strand, within AO356_29505 at 6862.233 kb on - strand, within AO356_29505 at 6862.233 kb on - strand, within AO356_29505 at 6862.267 kb on + strand, within AO356_29505 at 6862.267 kb on + strand, within AO356_29505 at 6862.268 kb on - strand, within AO356_29505 at 6862.268 kb on - strand, within AO356_29505 at 6862.465 kb on - strand, within AO356_29505 at 6862.465 kb on - strand, within AO356_29505 at 6862.654 kb on + strand, within AO356_29510 at 6862.655 kb on - strand, within AO356_29510 at 6862.656 kb on + strand at 6862.656 kb on + strand, within AO356_29510 at 6862.656 kb on + strand, within AO356_29510 at 6862.657 kb on - strand, within AO356_29510 at 6862.657 kb on - strand, within AO356_29510 at 6862.657 kb on - strand, within AO356_29510 at 6862.716 kb on + strand, within AO356_29510 at 6862.716 kb on + strand, within AO356_29510 at 6862.717 kb on - strand, within AO356_29510 at 6862.717 kb on - strand, within AO356_29510 at 6862.722 kb on + strand, within AO356_29510 at 6862.723 kb on - strand, within AO356_29510 at 6862.796 kb on + strand, within AO356_29510 at 6862.877 kb on + strand, within AO356_29510 at 6862.878 kb on - strand, within AO356_29510 at 6862.878 kb on - strand, within AO356_29510 at 6863.046 kb on + strand, within AO356_29510 at 6863.162 kb on + strand, within AO356_29510 at 6863.271 kb on - strand at 6863.271 kb on - strand at 6863.271 kb on - strand at 6863.328 kb on + strand at 6863.328 kb on + strand at 6863.328 kb on + strand at 6863.328 kb on + strand at 6863.328 kb on + strand at 6863.329 kb on - strand at 6863.329 kb on - strand at 6863.329 kb on - strand at 6863.329 kb on - strand at 6863.333 kb on - strand at 6863.351 kb on + strand at 6863.351 kb on + strand at 6863.355 kb on + strand at 6863.355 kb on + strand at 6863.356 kb on - strand at 6863.356 kb on - strand at 6863.356 kb on - strand at 6863.356 kb on - strand at 6863.356 kb on - strand at 6863.584 kb on + strand, within AO356_29515 at 6863.585 kb on - strand, within AO356_29515 at 6864.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 6,861,584 + -0.4 6,861,584 + +0.6 6,861,612 - +0.6 6,861,765 + AO356_29500 0.44 -0.2 6,861,766 - AO356_29500 0.45 +0.2 6,861,766 - AO356_29500 0.45 +0.8 6,861,910 + AO356_29500 0.85 -0.0 6,861,910 + AO356_29500 0.85 +0.9 6,861,938 - -0.8 6,861,938 - +1.0 6,861,994 + +1.6 6,861,994 + -0.6 6,861,994 + -0.0 6,861,994 + -0.5 6,861,995 - +0.5 6,862,026 - -0.7 6,862,026 - +1.2 6,862,042 + +0.1 6,862,082 + +0.4 6,862,082 + +0.3 6,862,089 + -0.5 6,862,089 + +0.4 6,862,089 + +1.2 6,862,089 + -0.4 6,862,090 - -0.4 6,862,090 - +0.2 6,862,090 - +0.3 6,862,090 - -0.8 6,862,090 - +0.1 6,862,090 - -0.9 6,862,157 + -0.6 6,862,158 - +0.5 6,862,158 - +0.2 6,862,232 + AO356_29505 0.19 -0.1 6,862,233 - AO356_29505 0.19 -0.1 6,862,233 - AO356_29505 0.19 +1.0 6,862,233 - AO356_29505 0.19 -0.4 6,862,267 + AO356_29505 0.28 +1.0 6,862,267 + AO356_29505 0.28 +0.1 6,862,268 - AO356_29505 0.28 -0.8 6,862,268 - AO356_29505 0.28 +0.4 6,862,465 - AO356_29505 0.77 +0.9 6,862,465 - AO356_29505 0.77 +0.2 6,862,654 + AO356_29510 0.13 -0.8 6,862,655 - AO356_29510 0.13 +0.3 6,862,656 + +0.1 6,862,656 + AO356_29510 0.13 -0.5 6,862,656 + AO356_29510 0.13 -1.4 6,862,657 - AO356_29510 0.14 -0.2 6,862,657 - AO356_29510 0.14 +0.6 6,862,657 - AO356_29510 0.14 -0.4 6,862,716 + AO356_29510 0.22 -0.2 6,862,716 + AO356_29510 0.22 -0.4 6,862,717 - AO356_29510 0.22 -0.6 6,862,717 - AO356_29510 0.22 +0.3 6,862,722 + AO356_29510 0.23 +0.8 6,862,723 - AO356_29510 0.23 +1.2 6,862,796 + AO356_29510 0.33 -0.7 6,862,877 + AO356_29510 0.44 +0.1 6,862,878 - AO356_29510 0.44 -0.1 6,862,878 - AO356_29510 0.44 -0.4 6,863,046 + AO356_29510 0.68 -0.9 6,863,162 + AO356_29510 0.84 -0.4 6,863,271 - +0.5 6,863,271 - +0.4 6,863,271 - +0.4 6,863,328 + +0.9 6,863,328 + +0.5 6,863,328 + -0.1 6,863,328 + -0.0 6,863,328 + -0.4 6,863,329 - +0.8 6,863,329 - -0.1 6,863,329 - +0.5 6,863,329 - +0.1 6,863,333 - -1.8 6,863,351 + +0.9 6,863,351 + -0.6 6,863,355 + +1.8 6,863,355 + +0.4 6,863,356 - -0.4 6,863,356 - +1.6 6,863,356 - -0.4 6,863,356 - +0.7 6,863,356 - -0.4 6,863,584 + AO356_29515 0.31 -0.1 6,863,585 - AO356_29515 0.31 -0.8 6,864,206 - +0.2
Or see this region's nucleotide sequence