Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24985 and AO356_24990 are separated by 312 nucleotides AO356_24990 and AO356_24995 are separated by 128 nucleotides AO356_24995 and AO356_25000 are separated by 51 nucleotides AO356_25000 and AO356_25005 are separated by 320 nucleotides
AO356_24985: AO356_24985 - 3-oxoacyl-ACP reductase, at 5,661,567 to 5,662,301
_24985
AO356_24990: AO356_24990 - 4-oxalocrotonate tautomerase, at 5,662,614 to 5,662,820
_24990
AO356_24995: AO356_24995 - LysR family transcriptional regulator, at 5,662,949 to 5,663,872
_24995
AO356_25000: AO356_25000 - hypothetical protein, at 5,663,924 to 5,664,358
_25000
AO356_25005: AO356_25005 - LysR family transcriptional regulator, at 5,664,679 to 5,665,575
_25005
Position (kb)
5662
5663
5664 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5661.953 kb on - strand, within AO356_24985 at 5661.953 kb on - strand, within AO356_24985 at 5662.214 kb on + strand, within AO356_24985 at 5662.214 kb on + strand, within AO356_24985 at 5662.215 kb on - strand, within AO356_24985 at 5662.215 kb on - strand, within AO356_24985 at 5662.216 kb on + strand, within AO356_24985 at 5662.217 kb on - strand, within AO356_24985 at 5662.217 kb on - strand, within AO356_24985 at 5662.217 kb on - strand, within AO356_24985 at 5662.217 kb on - strand, within AO356_24985 at 5662.421 kb on + strand at 5662.615 kb on + strand at 5662.616 kb on - strand at 5662.670 kb on + strand, within AO356_24990 at 5662.670 kb on + strand, within AO356_24990 at 5662.882 kb on + strand at 5662.882 kb on + strand at 5662.882 kb on + strand at 5662.882 kb on + strand at 5662.882 kb on + strand at 5662.883 kb on - strand at 5662.883 kb on - strand at 5662.886 kb on + strand at 5662.887 kb on - strand at 5662.889 kb on - strand at 5662.986 kb on + strand at 5662.986 kb on + strand at 5662.986 kb on + strand at 5662.986 kb on + strand at 5662.986 kb on + strand at 5662.986 kb on + strand at 5662.987 kb on - strand at 5662.987 kb on - strand at 5662.987 kb on - strand at 5662.987 kb on - strand at 5662.987 kb on - strand at 5663.159 kb on + strand, within AO356_24995 at 5663.160 kb on - strand, within AO356_24995 at 5663.270 kb on + strand, within AO356_24995 at 5663.477 kb on + strand, within AO356_24995 at 5663.477 kb on + strand, within AO356_24995 at 5663.624 kb on + strand, within AO356_24995 at 5663.694 kb on - strand, within AO356_24995 at 5663.742 kb on + strand, within AO356_24995 at 5663.742 kb on + strand, within AO356_24995 at 5663.742 kb on + strand, within AO356_24995 at 5663.743 kb on - strand, within AO356_24995 at 5663.743 kb on - strand, within AO356_24995 at 5663.777 kb on - strand, within AO356_24995 at 5663.786 kb on + strand at 5663.787 kb on - strand at 5664.186 kb on + strand, within AO356_25000 at 5664.186 kb on + strand, within AO356_25000 at 5664.187 kb on - strand, within AO356_25000 at 5664.187 kb on - strand, within AO356_25000 at 5664.187 kb on - strand, within AO356_25000 at 5664.233 kb on - strand, within AO356_25000 at 5664.310 kb on - strand, within AO356_25000 at 5664.379 kb on - strand at 5664.379 kb on - strand at 5664.379 kb on - strand at 5664.393 kb on + strand at 5664.394 kb on - strand at 5664.726 kb on + strand at 5664.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,661,953 - AO356_24985 0.53 -0.2 5,661,953 - AO356_24985 0.53 -0.0 5,662,214 + AO356_24985 0.88 +1.4 5,662,214 + AO356_24985 0.88 +0.6 5,662,215 - AO356_24985 0.88 +0.3 5,662,215 - AO356_24985 0.88 +0.1 5,662,216 + AO356_24985 0.88 -0.1 5,662,217 - AO356_24985 0.88 +0.0 5,662,217 - AO356_24985 0.88 -0.2 5,662,217 - AO356_24985 0.88 +0.1 5,662,217 - AO356_24985 0.88 -0.2 5,662,421 + -1.3 5,662,615 + -0.8 5,662,616 - -1.4 5,662,670 + AO356_24990 0.27 -1.1 5,662,670 + AO356_24990 0.27 -0.9 5,662,882 + -2.7 5,662,882 + -1.6 5,662,882 + -1.7 5,662,882 + -2.5 5,662,882 + -1.0 5,662,883 - -0.1 5,662,883 - -0.8 5,662,886 + -2.0 5,662,887 - -0.1 5,662,889 - +0.4 5,662,986 + -0.2 5,662,986 + +0.1 5,662,986 + +0.8 5,662,986 + +0.0 5,662,986 + +0.9 5,662,986 + +2.5 5,662,987 - -0.4 5,662,987 - +0.1 5,662,987 - +0.4 5,662,987 - +1.0 5,662,987 - +0.2 5,663,159 + AO356_24995 0.23 +0.1 5,663,160 - AO356_24995 0.23 +0.2 5,663,270 + AO356_24995 0.35 -0.4 5,663,477 + AO356_24995 0.57 -0.2 5,663,477 + AO356_24995 0.57 +0.2 5,663,624 + AO356_24995 0.73 -0.2 5,663,694 - AO356_24995 0.81 +0.6 5,663,742 + AO356_24995 0.86 -0.7 5,663,742 + AO356_24995 0.86 +0.2 5,663,742 + AO356_24995 0.86 +1.8 5,663,743 - AO356_24995 0.86 -0.5 5,663,743 - AO356_24995 0.86 +0.4 5,663,777 - AO356_24995 0.90 +0.3 5,663,786 + +0.6 5,663,787 - -0.3 5,664,186 + AO356_25000 0.60 -0.6 5,664,186 + AO356_25000 0.60 -0.6 5,664,187 - AO356_25000 0.60 +0.8 5,664,187 - AO356_25000 0.60 -0.4 5,664,187 - AO356_25000 0.60 -0.7 5,664,233 - AO356_25000 0.71 +0.4 5,664,310 - AO356_25000 0.89 -0.1 5,664,379 - +0.4 5,664,379 - +1.2 5,664,379 - +0.6 5,664,393 + -0.4 5,664,394 - +0.2 5,664,726 + +0.2 5,664,727 - -0.4
Or see this region's nucleotide sequence