Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21270 and AO356_21275 overlap by 1 nucleotides AO356_21275 and AO356_21280 overlap by 1 nucleotides AO356_21280 and AO356_21285 overlap by 4 nucleotides AO356_21285 and AO356_21290 are separated by 238 nucleotides
AO356_21270: AO356_21270 - peptidase, at 4,773,300 to 4,773,608
_21270
AO356_21275: AO356_21275 - peptidase, at 4,773,608 to 4,773,919
_21275
AO356_21280: AO356_21280 - transcriptional regulator, at 4,773,919 to 4,774,587
_21280
AO356_21285: AO356_21285 - histidine kinase, at 4,774,584 to 4,775,897
_21285
AO356_21290: AO356_21290 - sodium:proton antiporter, at 4,776,136 to 4,777,455
_21290
Position (kb)
4774
4775
4776 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4773.603 kb on - strand at 4773.607 kb on - strand at 4773.607 kb on - strand at 4773.607 kb on - strand at 4773.607 kb on - strand at 4773.824 kb on - strand, within AO356_21275 at 4773.824 kb on - strand, within AO356_21275 at 4773.824 kb on - strand, within AO356_21275 at 4773.908 kb on - strand at 4773.908 kb on - strand at 4774.030 kb on + strand, within AO356_21280 at 4774.031 kb on - strand, within AO356_21280 at 4774.051 kb on + strand, within AO356_21280 at 4774.051 kb on + strand, within AO356_21280 at 4774.051 kb on + strand, within AO356_21280 at 4774.210 kb on + strand, within AO356_21280 at 4774.211 kb on - strand, within AO356_21280 at 4774.399 kb on + strand, within AO356_21280 at 4774.456 kb on + strand, within AO356_21280 at 4774.561 kb on + strand at 4774.562 kb on - strand at 4774.562 kb on - strand at 4774.797 kb on + strand, within AO356_21285 at 4774.821 kb on + strand, within AO356_21285 at 4774.822 kb on - strand, within AO356_21285 at 4775.547 kb on + strand, within AO356_21285 at 4775.547 kb on + strand, within AO356_21285 at 4775.547 kb on + strand, within AO356_21285 at 4775.547 kb on + strand, within AO356_21285 at 4775.547 kb on + strand, within AO356_21285 at 4775.547 kb on + strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.548 kb on - strand, within AO356_21285 at 4775.974 kb on - strand at 4776.131 kb on + strand at 4776.140 kb on + strand at 4776.141 kb on - strand at 4776.141 kb on - strand at 4776.141 kb on - strand at 4776.149 kb on + strand at 4776.149 kb on + strand at 4776.149 kb on + strand at 4776.150 kb on - strand at 4776.150 kb on - strand at 4776.320 kb on - strand, within AO356_21290 at 4776.538 kb on + strand, within AO356_21290 at 4776.538 kb on + strand, within AO356_21290 at 4776.538 kb on + strand, within AO356_21290 at 4776.610 kb on + strand, within AO356_21290 at 4776.610 kb on + strand, within AO356_21290 at 4776.610 kb on + strand, within AO356_21290 at 4776.611 kb on - strand, within AO356_21290 at 4776.611 kb on - strand, within AO356_21290 at 4776.611 kb on - strand, within AO356_21290 at 4776.611 kb on - strand, within AO356_21290 at 4776.611 kb on - strand, within AO356_21290 at 4776.611 kb on - strand, within AO356_21290 at 4776.639 kb on + strand, within AO356_21290 at 4776.640 kb on - strand, within AO356_21290 at 4776.640 kb on - strand, within AO356_21290 at 4776.790 kb on + strand, within AO356_21290 at 4776.790 kb on + strand, within AO356_21290 at 4776.835 kb on + strand, within AO356_21290 at 4776.836 kb on - strand, within AO356_21290 at 4776.836 kb on - strand, within AO356_21290
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 4,773,603 - -2.8 4,773,607 - -0.8 4,773,607 - -1.2 4,773,607 - -1.6 4,773,607 - -0.8 4,773,824 - AO356_21275 0.69 -2.2 4,773,824 - AO356_21275 0.69 +0.8 4,773,824 - AO356_21275 0.69 -2.6 4,773,908 - -1.4 4,773,908 - -0.8 4,774,030 + AO356_21280 0.17 -0.3 4,774,031 - AO356_21280 0.17 +0.4 4,774,051 + AO356_21280 0.20 +0.7 4,774,051 + AO356_21280 0.20 +0.1 4,774,051 + AO356_21280 0.20 -0.6 4,774,210 + AO356_21280 0.43 +1.1 4,774,211 - AO356_21280 0.44 -0.8 4,774,399 + AO356_21280 0.72 +0.3 4,774,456 + AO356_21280 0.80 -0.8 4,774,561 + +0.1 4,774,562 - -0.5 4,774,562 - +0.4 4,774,797 + AO356_21285 0.16 -1.1 4,774,821 + AO356_21285 0.18 -2.7 4,774,822 - AO356_21285 0.18 -0.9 4,775,547 + AO356_21285 0.73 -1.5 4,775,547 + AO356_21285 0.73 -1.2 4,775,547 + AO356_21285 0.73 -1.8 4,775,547 + AO356_21285 0.73 -0.6 4,775,547 + AO356_21285 0.73 +0.2 4,775,547 + AO356_21285 0.73 -1.0 4,775,548 - AO356_21285 0.73 -1.6 4,775,548 - AO356_21285 0.73 -0.4 4,775,548 - AO356_21285 0.73 -3.2 4,775,548 - AO356_21285 0.73 -1.2 4,775,548 - AO356_21285 0.73 -1.8 4,775,548 - AO356_21285 0.73 +0.3 4,775,548 - AO356_21285 0.73 +0.3 4,775,548 - AO356_21285 0.73 -1.4 4,775,974 - +0.2 4,776,131 + +0.8 4,776,140 + +0.0 4,776,141 - +0.4 4,776,141 - -0.8 4,776,141 - +2.8 4,776,149 + -0.6 4,776,149 + +0.8 4,776,149 + +0.1 4,776,150 - -0.2 4,776,150 - -0.5 4,776,320 - AO356_21290 0.14 +0.4 4,776,538 + AO356_21290 0.30 +0.4 4,776,538 + AO356_21290 0.30 +0.4 4,776,538 + AO356_21290 0.30 +0.6 4,776,610 + AO356_21290 0.36 +0.6 4,776,610 + AO356_21290 0.36 +0.0 4,776,610 + AO356_21290 0.36 -1.1 4,776,611 - AO356_21290 0.36 -0.8 4,776,611 - AO356_21290 0.36 +0.3 4,776,611 - AO356_21290 0.36 +0.1 4,776,611 - AO356_21290 0.36 +1.2 4,776,611 - AO356_21290 0.36 +0.5 4,776,611 - AO356_21290 0.36 +0.1 4,776,639 + AO356_21290 0.38 +0.1 4,776,640 - AO356_21290 0.38 -0.8 4,776,640 - AO356_21290 0.38 +0.3 4,776,790 + AO356_21290 0.50 +0.2 4,776,790 + AO356_21290 0.50 +0.4 4,776,835 + AO356_21290 0.53 -0.9 4,776,836 - AO356_21290 0.53 -0.2 4,776,836 - AO356_21290 0.53 +0.4
Or see this region's nucleotide sequence