Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_01435

Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_01425 and AO356_01430 are separated by 13 nucleotidesAO356_01430 and AO356_01435 are separated by 142 nucleotidesAO356_01435 and AO356_01440 are separated by 98 nucleotidesAO356_01440 and AO356_01445 are separated by 76 nucleotides AO356_01425: AO356_01425 - LysR family transcriptional regulator, at 326,848 to 327,780 _01425 AO356_01430: AO356_01430 - NADP oxidoreductase, at 327,794 to 328,396 _01430 AO356_01435: AO356_01435 - LysR family transcriptional regulator, at 328,539 to 329,468 _01435 AO356_01440: AO356_01440 - hypothetical protein, at 329,567 to 330,331 _01440 AO356_01445: AO356_01445 - hypothetical protein, at 330,408 to 330,995 _01445 Position (kb) 328 329 330Strain fitness (log2 ratio) -1 0 1 2at 327.644 kb on - strand, within AO356_01425at 327.881 kb on + strand, within AO356_01430at 327.882 kb on - strand, within AO356_01430at 328.316 kb on + strand, within AO356_01430at 328.316 kb on + strand, within AO356_01430at 328.316 kb on + strand, within AO356_01430at 328.317 kb on - strand, within AO356_01430at 328.317 kb on - strand, within AO356_01430at 328.317 kb on - strand, within AO356_01430at 328.428 kb on + strandat 328.521 kb on + strandat 328.521 kb on + strandat 328.521 kb on + strandat 328.522 kb on - strandat 328.525 kb on - strandat 328.858 kb on + strand, within AO356_01435at 329.049 kb on + strand, within AO356_01435at 329.328 kb on + strand, within AO356_01435at 329.328 kb on + strand, within AO356_01435at 329.328 kb on + strand, within AO356_01435at 329.328 kb on + strand, within AO356_01435at 329.329 kb on - strand, within AO356_01435at 329.329 kb on - strand, within AO356_01435at 329.329 kb on - strand, within AO356_01435at 329.356 kb on - strand, within AO356_01435at 329.356 kb on - strand, within AO356_01435at 329.358 kb on + strand, within AO356_01435at 329.359 kb on - strand, within AO356_01435at 329.359 kb on - strand, within AO356_01435at 329.533 kb on - strandat 329.789 kb on + strand, within AO356_01440at 329.789 kb on + strand, within AO356_01440at 329.789 kb on + strand, within AO356_01440at 329.790 kb on - strand, within AO356_01440at 329.790 kb on - strand, within AO356_01440at 329.939 kb on + strand, within AO356_01440at 329.940 kb on - strand, within AO356_01440at 329.940 kb on - strand, within AO356_01440at 330.003 kb on + strand, within AO356_01440at 330.004 kb on - strand, within AO356_01440at 330.004 kb on - strand, within AO356_01440at 330.188 kb on + strand, within AO356_01440at 330.188 kb on + strand, within AO356_01440at 330.188 kb on + strand, within AO356_01440at 330.188 kb on + strand, within AO356_01440at 330.188 kb on + strand, within AO356_01440at 330.189 kb on - strand, within AO356_01440at 330.189 kb on - strand, within AO356_01440at 330.189 kb on - strand, within AO356_01440at 330.189 kb on - strand, within AO356_01440at 330.189 kb on - strand, within AO356_01440at 330.189 kb on - strand, within AO356_01440at 330.423 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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327,644 - AO356_01425 0.85 +0.3
327,881 + AO356_01430 0.14 -0.0
327,882 - AO356_01430 0.15 +0.0
328,316 + AO356_01430 0.87 +0.9
328,316 + AO356_01430 0.87 -0.3
328,316 + AO356_01430 0.87 +0.7
328,317 - AO356_01430 0.87 -0.0
328,317 - AO356_01430 0.87 +0.1
328,317 - AO356_01430 0.87 -0.3
328,428 + -0.7
328,521 + -0.1
328,521 + +0.9
328,521 + -0.6
328,522 - -0.1
328,525 - +0.6
328,858 + AO356_01435 0.34 +0.3
329,049 + AO356_01435 0.55 -0.1
329,328 + AO356_01435 0.85 +0.1
329,328 + AO356_01435 0.85 -0.3
329,328 + AO356_01435 0.85 -1.4
329,328 + AO356_01435 0.85 -0.3
329,329 - AO356_01435 0.85 -0.7
329,329 - AO356_01435 0.85 -0.1
329,329 - AO356_01435 0.85 +0.1
329,356 - AO356_01435 0.88 +0.1
329,356 - AO356_01435 0.88 +0.6
329,358 + AO356_01435 0.88 +1.3
329,359 - AO356_01435 0.88 +0.2
329,359 - AO356_01435 0.88 -0.1
329,533 - +0.4
329,789 + AO356_01440 0.29 -1.5
329,789 + AO356_01440 0.29 -0.4
329,789 + AO356_01440 0.29 +0.9
329,790 - AO356_01440 0.29 +0.8
329,790 - AO356_01440 0.29 -0.1
329,939 + AO356_01440 0.49 -0.2
329,940 - AO356_01440 0.49 +0.3
329,940 - AO356_01440 0.49 +0.9
330,003 + AO356_01440 0.57 -0.4
330,004 - AO356_01440 0.57 -0.0
330,004 - AO356_01440 0.57 +0.3
330,188 + AO356_01440 0.81 +0.6
330,188 + AO356_01440 0.81 -1.3
330,188 + AO356_01440 0.81 +2.3
330,188 + AO356_01440 0.81 -1.5
330,188 + AO356_01440 0.81 +0.5
330,189 - AO356_01440 0.81 +1.1
330,189 - AO356_01440 0.81 -0.9
330,189 - AO356_01440 0.81 +0.3
330,189 - AO356_01440 0.81 +0.1
330,189 - AO356_01440 0.81 -0.3
330,189 - AO356_01440 0.81 +0.3
330,423 - -0.9

Or see this region's nucleotide sequence