Experiment: Varel_Bryant_medium_Glucose with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1977 and BT1978 are separated by 68 nucleotides BT1978 and BT1979 are separated by 178 nucleotides BT1979 and BT1980 are separated by 146 nucleotides
BT1977: BT1977 - hypothetical protein (NCBI ptt file), at 2,487,583 to 2,488,572
BT1977
BT1978: BT1978 - Holliday junction DNA helicase ruvA (NCBI ptt file), at 2,488,641 to 2,489,246
BT1978
BT1979: BT1979 - Meso-diaminopimelate D-dehydrogenase (NCBI ptt file), at 2,489,425 to 2,490,324
BT1979
BT1980: BT1980 - transposase (NCBI ptt file), at 2,490,471 to 2,492,132
BT1980
Position (kb)
2489
2490
2491 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2488.440 kb on + strand, within BT1977 at 2488.441 kb on - strand, within BT1977 at 2488.443 kb on - strand, within BT1977 at 2488.444 kb on + strand, within BT1977 at 2488.617 kb on - strand at 2488.617 kb on - strand at 2488.617 kb on - strand at 2488.620 kb on - strand at 2488.630 kb on + strand at 2488.630 kb on + strand at 2488.631 kb on - strand at 2488.631 kb on - strand at 2488.634 kb on - strand at 2488.634 kb on - strand at 2488.643 kb on - strand at 2489.147 kb on + strand, within BT1978 at 2489.446 kb on - strand at 2489.473 kb on + strand at 2489.943 kb on + strand, within BT1979 at 2489.973 kb on - strand, within BT1979 at 2489.973 kb on - strand, within BT1979 at 2489.974 kb on + strand, within BT1979 at 2489.974 kb on + strand at 2489.974 kb on + strand, within BT1979 at 2489.975 kb on - strand, within BT1979 at 2489.976 kb on + strand, within BT1979 at 2490.037 kb on + strand, within BT1979 at 2490.038 kb on - strand, within BT1979 at 2490.038 kb on - strand, within BT1979 at 2490.204 kb on - strand, within BT1979 at 2490.250 kb on + strand at 2490.260 kb on + strand at 2490.260 kb on + strand at 2490.321 kb on - strand at 2490.323 kb on - strand at 2490.427 kb on + strand at 2490.431 kb on - strand at 2490.481 kb on + strand at 2490.568 kb on + strand at 2490.569 kb on - strand at 2490.741 kb on + strand, within BT1980 at 2490.743 kb on + strand, within BT1980 at 2490.818 kb on - strand, within BT1980 at 2490.818 kb on - strand, within BT1980 at 2490.826 kb on + strand, within BT1980 at 2490.827 kb on - strand, within BT1980 at 2490.881 kb on - strand, within BT1980 at 2490.888 kb on - strand, within BT1980 at 2491.186 kb on - strand, within BT1980
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Cephalothin 0.1 mM remove 2,488,440 + BT1977 0.87 -0.6 2,488,441 - BT1977 0.87 -1.0 2,488,443 - BT1977 0.87 -0.1 2,488,444 + BT1977 0.87 -1.3 2,488,617 - -1.3 2,488,617 - -2.3 2,488,617 - +1.6 2,488,620 - -1.0 2,488,630 + -1.5 2,488,630 + -1.0 2,488,631 - -0.3 2,488,631 - -2.0 2,488,634 - -0.4 2,488,634 - -1.7 2,488,643 - -1.3 2,489,147 + BT1978 0.83 -1.6 2,489,446 - -3.0 2,489,473 + -2.9 2,489,943 + BT1979 0.58 -2.0 2,489,973 - BT1979 0.61 -2.6 2,489,973 - BT1979 0.61 -1.7 2,489,974 + BT1979 0.61 -2.0 2,489,974 + -1.6 2,489,974 + BT1979 0.61 -0.0 2,489,975 - BT1979 0.61 -1.9 2,489,976 + BT1979 0.61 -2.2 2,490,037 + BT1979 0.68 -1.9 2,490,038 - BT1979 0.68 -3.8 2,490,038 - BT1979 0.68 -0.0 2,490,204 - BT1979 0.87 +1.0 2,490,250 + -2.5 2,490,260 + -0.0 2,490,260 + -2.0 2,490,321 - -0.2 2,490,323 - -0.0 2,490,427 + -3.0 2,490,431 - -0.2 2,490,481 + -0.3 2,490,568 + -1.6 2,490,569 - -0.3 2,490,741 + BT1980 0.16 -0.8 2,490,743 + BT1980 0.16 -0.0 2,490,818 - BT1980 0.21 +1.6 2,490,818 - BT1980 0.21 -0.0 2,490,826 + BT1980 0.21 -1.0 2,490,827 - BT1980 0.21 -1.0 2,490,881 - BT1980 0.25 +0.1 2,490,888 - BT1980 0.25 -1.0 2,491,186 - BT1980 0.43 -1.6
Or see this region's nucleotide sequence