Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23445 and HSERO_RS23450 overlap by 1 nucleotides HSERO_RS23450 and HSERO_RS23455 are separated by 77 nucleotides HSERO_RS23455 and HSERO_RS23460 are separated by 22 nucleotides
HSERO_RS23445: HSERO_RS23445 - 2-hydroxychromene-2-carboxylate isomerase, at 5,378,219 to 5,378,821
_RS23445
HSERO_RS23450: HSERO_RS23450 - hypothetical protein, at 5,378,821 to 5,379,231
_RS23450
HSERO_RS23455: HSERO_RS23455 - methylcrotonoyl-CoA carboxylase, at 5,379,309 to 5,380,943
_RS23455
HSERO_RS23460: HSERO_RS23460 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 5,380,966 to 5,382,981
_RS23460
Position (kb) 5379
5380
5381 Strain fitness (log2 ratio) -2
-1
0
1 at 5378.389 kb on + strand, within HSERO_RS23445 at 5378.553 kb on - strand, within HSERO_RS23445 at 5379.046 kb on + strand, within HSERO_RS23450 at 5379.054 kb on - strand, within HSERO_RS23450 at 5379.641 kb on - strand, within HSERO_RS23455 at 5380.207 kb on - strand, within HSERO_RS23455 at 5380.668 kb on - strand, within HSERO_RS23455 at 5380.970 kb on - strand at 5381.140 kb on + strand at 5381.186 kb on - strand, within HSERO_RS23460 at 5381.186 kb on - strand, within HSERO_RS23460 at 5381.186 kb on - strand, within HSERO_RS23460 at 5381.237 kb on - strand, within HSERO_RS23460 at 5381.239 kb on + strand, within HSERO_RS23460 at 5381.252 kb on - strand, within HSERO_RS23460 at 5381.377 kb on + strand, within HSERO_RS23460 at 5381.377 kb on + strand, within HSERO_RS23460 at 5381.627 kb on + strand, within HSERO_RS23460 at 5381.635 kb on - strand, within HSERO_RS23460 at 5381.917 kb on + strand, within HSERO_RS23460
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 5,378,389 + HSERO_RS23445 0.28 -0.4 5,378,553 - HSERO_RS23445 0.55 -0.1 5,379,046 + HSERO_RS23450 0.55 -1.0 5,379,054 - HSERO_RS23450 0.57 +0.6 5,379,641 - HSERO_RS23455 0.20 +1.5 5,380,207 - HSERO_RS23455 0.55 +0.6 5,380,668 - HSERO_RS23455 0.83 +0.1 5,380,970 - -0.8 5,381,140 + +0.4 5,381,186 - HSERO_RS23460 0.11 +0.0 5,381,186 - HSERO_RS23460 0.11 -1.1 5,381,186 - HSERO_RS23460 0.11 -1.0 5,381,237 - HSERO_RS23460 0.13 -2.3 5,381,239 + HSERO_RS23460 0.14 -0.4 5,381,252 - HSERO_RS23460 0.14 +0.3 5,381,377 + HSERO_RS23460 0.20 +0.5 5,381,377 + HSERO_RS23460 0.20 +0.3 5,381,627 + HSERO_RS23460 0.33 -0.3 5,381,635 - HSERO_RS23460 0.33 -0.1 5,381,917 + HSERO_RS23460 0.47 +0.3
Or see this region's nucleotide sequence