Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22065 and HSERO_RS22070 are separated by 66 nucleotides HSERO_RS22070 and HSERO_RS22075 are separated by 3 nucleotides HSERO_RS22075 and HSERO_RS22080 are separated by 11 nucleotides
HSERO_RS22065: HSERO_RS22065 - EpiA, at 5,044,350 to 5,045,303
_RS22065
HSERO_RS22070: HSERO_RS22070 - CDP-4-keto-6-deoxy-D-glucose-3-dehydrase, at 5,045,370 to 5,046,542
_RS22070
HSERO_RS22075: HSERO_RS22075 - phosphohexose mutase, at 5,046,546 to 5,047,268
_RS22075
HSERO_RS22080: HSERO_RS22080 - phosphoheptose isomerase, at 5,047,280 to 5,047,852
_RS22080
Position (kb) 5045
5046
5047 Strain fitness (log2 ratio) -1
0
1
2 at 5044.526 kb on - strand, within HSERO_RS22065 at 5045.152 kb on - strand, within HSERO_RS22065 at 5045.202 kb on - strand, within HSERO_RS22065 at 5045.255 kb on - strand at 5045.352 kb on - strand at 5045.489 kb on - strand, within HSERO_RS22070 at 5045.569 kb on - strand, within HSERO_RS22070 at 5045.774 kb on - strand, within HSERO_RS22070 at 5045.796 kb on + strand, within HSERO_RS22070 at 5045.870 kb on - strand, within HSERO_RS22070 at 5045.885 kb on - strand, within HSERO_RS22070 at 5045.930 kb on - strand, within HSERO_RS22070 at 5045.976 kb on - strand, within HSERO_RS22070 at 5046.591 kb on + strand at 5046.625 kb on - strand, within HSERO_RS22075 at 5046.707 kb on + strand, within HSERO_RS22075 at 5046.755 kb on + strand, within HSERO_RS22075 at 5046.950 kb on + strand, within HSERO_RS22075 at 5046.963 kb on - strand, within HSERO_RS22075 at 5047.249 kb on + strand at 5047.257 kb on - strand at 5047.257 kb on - strand at 5047.272 kb on + strand at 5047.446 kb on + strand, within HSERO_RS22080
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 5,044,526 - HSERO_RS22065 0.18 -1.1 5,045,152 - HSERO_RS22065 0.84 +0.2 5,045,202 - HSERO_RS22065 0.89 -0.7 5,045,255 - +0.4 5,045,352 - -0.6 5,045,489 - HSERO_RS22070 0.10 +0.4 5,045,569 - HSERO_RS22070 0.17 +1.8 5,045,774 - HSERO_RS22070 0.34 -0.8 5,045,796 + HSERO_RS22070 0.36 -0.1 5,045,870 - HSERO_RS22070 0.43 -0.2 5,045,885 - HSERO_RS22070 0.44 +0.5 5,045,930 - HSERO_RS22070 0.48 +1.5 5,045,976 - HSERO_RS22070 0.52 +0.4 5,046,591 + -0.1 5,046,625 - HSERO_RS22075 0.11 -0.3 5,046,707 + HSERO_RS22075 0.22 +0.0 5,046,755 + HSERO_RS22075 0.29 +0.4 5,046,950 + HSERO_RS22075 0.56 -0.4 5,046,963 - HSERO_RS22075 0.58 +0.6 5,047,249 + -0.4 5,047,257 - +1.4 5,047,257 - -1.3 5,047,272 + -0.9 5,047,446 + HSERO_RS22080 0.29 -0.6
Or see this region's nucleotide sequence